The impact of the number of cores on tissue microarray studies investigating prostate cancer biomarkers

  • Authors:
    • Pierre Tennstedt
    • Patricia Köster
    • Andreas Brüchmann
    • Martina Mirlacher
    • Alexander Haese
    • Thomas Steuber
    • Guido Sauter
    • Hartwig Huland
    • Markus Graefen
    • Thorsten Schlomm
    • Sarah Minner
    • Ronald Simon
  • View Affiliations

  • Published online on: September 28, 2011     https://doi.org/10.3892/ijo.2011.1216
  • Pages: 261-268
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Most tissue microarray studies have used a single 0.6-mm tissue core per donor tissue. It has been suggested that multiple cores per donor can increase the representativity of tissue microarray studies. To estimate the potential benefit of multiple cores, we analyzed Ki67 and p53 in triplet cores taken from three different areas of 3,261 prostate cancer tissue blocks. Both p53 and Ki67 labeling index were linked to advanced tumor stage (p<0.0001 each), Gleason score (p<0.0001), and early PSA recurrence (p<0.0001) independently of whether the 3 tissue spots were analyzed separately or combined for a consensus result. The rate of positive findings increased with the amount of analyzed tissue. The average Ki67 labeling index was higher in tumors with 3 interpretable spots (5.3±5.6) as compared to two (4.1±4.7) or one interpretable spot (4.1±4.2, p<0.0001). For p53, tumors with three interpretable spots were positive in 3.8% of cases, and tumors with 1 or 2 interpretable spots in 1.9% only (p=0.003). These data demonstrate that using multiple cores in a tissue microarray does not necessarily increase the ability to identify associations of biomarkers with tumor phenotype and prognosis but has always the disadvantage of additional work and tissue requirements. Multiple cores may even lead to statistical problems if unequal amounts of tissue are analyzed per tumor.

Related Articles

Journal Cover

January 2012
Volume 40 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tennstedt P, Köster P, Brüchmann A, Mirlacher M, Haese A, Steuber T, Sauter G, Huland H, Graefen M, Schlomm T, Schlomm T, et al: The impact of the number of cores on tissue microarray studies investigating prostate cancer biomarkers. Int J Oncol 40: 261-268, 2012
APA
Tennstedt, P., Köster, P., Brüchmann, A., Mirlacher, M., Haese, A., Steuber, T. ... Simon, R. (2012). The impact of the number of cores on tissue microarray studies investigating prostate cancer biomarkers. International Journal of Oncology, 40, 261-268. https://doi.org/10.3892/ijo.2011.1216
MLA
Tennstedt, P., Köster, P., Brüchmann, A., Mirlacher, M., Haese, A., Steuber, T., Sauter, G., Huland, H., Graefen, M., Schlomm, T., Minner, S., Simon, R."The impact of the number of cores on tissue microarray studies investigating prostate cancer biomarkers". International Journal of Oncology 40.1 (2012): 261-268.
Chicago
Tennstedt, P., Köster, P., Brüchmann, A., Mirlacher, M., Haese, A., Steuber, T., Sauter, G., Huland, H., Graefen, M., Schlomm, T., Minner, S., Simon, R."The impact of the number of cores on tissue microarray studies investigating prostate cancer biomarkers". International Journal of Oncology 40, no. 1 (2012): 261-268. https://doi.org/10.3892/ijo.2011.1216