Published online Jun 30, 2010.
https://doi.org/10.3947/ic.2010.42.3.162
Genotype of Varicella Zoster Virus Isolated from Korean Elderly Patients with Herpes Zoster
Abstract
Background
Herpes zoster develops via reactivation of the latent varicella zoster virus (VZV) in neuronal ganglia as host immunity declines. In Korea, seroprevalence of VZV is very high and population at risk for herpes zoster is increasing. The goal of this study is to evaluate the infection rate of the VZV and the lifetime prevalence of herpes zoster, and to determine the genotype of VZV.
Materials and Methods
Serum IgG antibody titer was measured in 399 patients. Lifetime prevalence of herpes zoster was evaluated through a survey of 2,054 participants. VZV was isolated by cell culture technique using MRC-5 cells. To determine the genotype of VZV, ORF 22, 38, 54, 62 were amplified by PCR, and after digestion of the PCR products with enzymes pstI, bglI, and smaI, restriction-fragment-length-polymorphism (RFLP) was analysed. The amplified ORF 22 PCR product was sequenced and checked for single nucleotide polymorphisms.
Results
The overall seroprevalence of VZV IgG in adults was 93.9% (375/399). The overall lifetime prevalence of herpes zoster was 13.7% (282/2,054). Of the patients with herpes zoster, 17.7% (50/282) of patients experienced postherpetic neuralgia for more than one month. All 22 VZV isolates were of J genotype; 21 (95.4%) isolates were all pstI+ bglI+ smaI-, and 1 (4.5%) isolate was pstI- bglI+ smaI- (pOka) genotype.
Conclusions
The seroprevalece of VZV IgG antibody was 93.9% in adults. The lifetime prevalence of herpes zoster was 13.7%. The genotype of VZV isolated from adults over 60 years old were all of J genotype.
Figure 1
RFLP Pattern of ORF 38, 54, and 62 Product Digested with pstI, bglI, smaI. A) A 350bp ORF 38 product was digested with pstI. The product yielded 2 fragments of 250bp and 100bp in size. B) Digestion of 268bp ORF 62 product with smaI yielded 3 fragments 153bp, 79bp, 36bp in size. C) Digestion of 222bp ORF 54 product with bglI yielded 2 fragments 137bp and 87bp in size.
Table 1
Sequences of Primers Used to Amplify Sites ORF 22, 38, 54, and 62
Table 2
Prevalence and Mean titer of VZV IgG Antibody according to Age Groups
Table 3
Prevalence of Herpes Zoster according to Age Group and Sex
Table 4
Prevalence and Duration of Postherpetic Neuralgia in 282 Patients with a History of Herpes Zoster
Table 5
RFLP Analysis and Final Genotype of VZV Isolated from 22 Elderly Korean Patients
Table 6
SNP Analysis of ORF 22 in VZV Isolated from 22 Elderly Korean Patients
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