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CC BY-NC-ND 4.0 · Eur J Dent 2014; 08(04): 509-514
DOI: 10.4103/1305-7456.143634
Original Article
Dental Investigation Society

Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: An in vitro study

Liliana Guimarães Oliveira Ricatto
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
,
Luis Augusto Lopato Conrado
2   Department of Biomedical Engineering, Unicastelo, São José dos Campos, São Paulo, Brazil
,
Cecilia Pedroso Turssi
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
,
Fabiana Mantovani Gomes França
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
,
Roberta Tarkany Basting
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
,
Flávia Lucisano Botelho Amaral
1   Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil
› Author Affiliations
Further Information

Publication History

Publication Date:
25 September 2019 (online)

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ABSTRACT

Objective: The aim of this study was to evaluate in vitro the effect of photodynamic therapy (PDT) using LASER or light emitting diode (LED) on cariogenic bacteria (Streptococcus mutans [SM] and Lactobacillus casei [LC]) in bovine dentin. Materials and Methods: Twenty five fragments of dentin were contaminated with SM and LC strands and divided into five experimental groups according to the therapy they received (n = 5): C – control (no treatment), SCLED – no dye/LED application (94 J/cm2), SCLASER – no dye/LASER application (94 J/cm2), CCLED – dye/LED application (94 J/cm2) and CCLASER – dye/LASER application (94 J/cm2). The dye used was methylene blue at 10 mM. Dentin scrapes were harvested from each fragment and prepared for counts of colony forming units (CFU)/mL. The data were analyzed using Kruskal–Wallis, followed by Student–Newman–Keuls (⍺ =0.05). Results: Regarding SM, groups CCLASER and CCLED showed a significant reduction in CFU/mL, which was statistically superior to the SCLASER, SCLED and C groups. Regarding LC, the groups CCLASER and CCLED caused a significant reduction in CFU/mL when compared with SCLASER, which showed intermediate values. SCLED and C had a lesser effect on reducing CFU/mL, where the former showed values similar to those of SCLASER. Conclusions: In conclusion, PDT combined with LASER or LED and methylene blue had a significant antimicrobial effect on cariogenic bacteria in the dentin.

Key words:

Lactobacillus casei - photodynamic therapy - semi-conductor lasers - Streptococcus mutans
 

  • REFERENCES

  • 1 Baptista A, Kato IT, Prates RA, Suzuki LC, Raele MP, Freitas AZ. et al. Antimicrobial photodynamic therapy as a strategy to arrest enamel demineralization: A short-term study on incipient caries in a rat model. Photochem Photobiol 2012; 88: 584-9
    CrossrefPubMedGoogle Scholar
  • 2 Wilson BC, Patterson MS. The physics, biophysics and technology of photodynamic therapy. Phys Med Biol 2008; 53: R61-109
    CrossrefPubMedGoogle Scholar
  • 3 Biel MA. Photodynamic therapy in head and neck cancer. Curr Oncol Rep 2002; 4: 87-96
    CrossrefPubMedGoogle Scholar
  • 4 Plaetzer K, Krammer B, Berlanda J, Berr F, Kiesslich T. Photophysics and photochemistry of photodynamic therapy: Fundamental aspects. Lasers Med Sci 2009; 24: 259-68
    CrossrefPubMedGoogle Scholar
  • 5 Hamblin MR, Hasan T. Photodynamic therapy: A new antimicrobial approach to infectious disease?. Photochem Photobiol Sci 2004; 3: 436-50
    CrossrefPubMedGoogle Scholar
  • 6 Kubler AC. Photodynamic therapy. Med Laser Appl 2005; 20: 37-45
    CrossrefGoogle Scholar
  • 7 Konopka K, Goslinski T. Photodynamic therapy in dentistry. J Dent Res 2007; 86: 694-707
    CrossrefPubMedGoogle Scholar
  • 8 Komerik N, MacRobert AJ. Photodynamic therapy as an alternative antimicrobial modality for oral infections. J Environ Pathol Toxicol Oncol 2006; 25: 487-504
    CrossrefPubMedGoogle Scholar
  • 9 Zanin IC, Gonçalves RB, Junior AB, Hope CK, Pratten J. Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: An in vitro study. J Antimicrob Chemother 2005; 56: 324-30
    CrossrefPubMedGoogle Scholar
  • 10 Zanin IC, Lobo MM, Rodrigues LK, Pimenta LA, Höfling JF, Gonçalves RB. Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode. Eur J Oral Sci 2006; 114: 64-9
    PubMedGoogle Scholar
  • 11 Wood S, Metcalf D, Devine D, Robinson C. Erythrosine is a potential photosensitizer for the photodynamic therapy of oral plaque biofilms. J Antimicrob Chemother 2006; 57: 680-4
    CrossrefPubMedGoogle Scholar
  • 12 de Oliveira BP, Aguiar CM, Camara AC. Photodynamic therapy in combating the causative microorganisms from endodontic infections. Eur J Dent 2014; 8: 424-30
    Article in Thieme ConnectPubMedGoogle Scholar
  • 13 Yildirim C, Karaarslan ES, Ozsevik S, Zer Y, Sari T, Usumez A. Antimicrobial efficiency of photodynamic therapy with different irradiation durations. Eur J Dent 2013; 7: 469-73
    Article in Thieme ConnectPubMedGoogle Scholar
  • 14 Nagata JY, Hioka N, Kimura E, Batistela VR, Terada RS, Graciano AX. et al. Antibacterial photodynamic therapy for dental caries: Evaluation of the photosensitizers used and light source properties. Photodiagnosis Photodyn Ther 2012; 9: 122-31
    CrossrefPubMedGoogle Scholar
  • 15 Mang TS, Tayal DP, Baier R. Photodynamic therapy as an alternative treatment for disinfection of bacteria in oral biofilms. Lasers Surg Med 2012; 44: 588-96
    CrossrefPubMedGoogle Scholar
  • 16 Teixeira AH, Pereira ES, Rodrigues LK, Saxena D, Duarte S, Zanin IC. Effect of photodynamic antimicrobial chemotherapy on in vitro and in situ biofilms. Caries Res 2012; 46: 549-54
    CrossrefPubMedGoogle Scholar
  • 17 Lima JP, Sampaio de MeloMA, Borges FM, Teixeira AH, Steiner-Oliveira C, Nobre Dos SantosM. et al. Evaluation of the antimicrobial effect of photodynamic antimicrobial therapy in an in situ model of dentine caries. Eur J Oral Sci 2009; 117: 568-74
    CrossrefPubMedGoogle Scholar
  • 18 de Melo MA, Rolim JP, Zanin IC, Barros EB, da-Costa EF, Rodrigues LK. Characterization of antimicrobial photodynamic therapy-treated Streptococci mutans: An atomic force microscopy study. Photomed Laser Surg 2013; 31: 105-9
    CrossrefPubMedGoogle Scholar
  • 19 Guglielmi CdeA, Simionato MR, Ramalho KM, Imparato JC, Pinheiro SL, Luz MA. Clinical use of photodynamic antimicrobial chemotherapy for the treatment of deep carious lesions. J Biomed Opt 2011; 16: 088003
    CrossrefPubMedGoogle Scholar
  • 20 Dobson J, Wilson M. Sensitization of oral bacteria in biofilms to killing by light from a low-power laser. Arch Oral Biol 1992; 37: 883-7
    CrossrefPubMedGoogle Scholar
  • 21 Okamoto H, Iwase T, Morioka T. Dye-mediated bactericidal effect of He-Ne laser irradiation on oral microorganisms. Lasers Surg Med 1992; 12: 450-8
    CrossrefPubMedGoogle Scholar
  • 22 Paulino TP, Magalhães PP, Thedei Jr G, Tedesco AC, Ciancaglini P. Use of visible light-based photodynamic therapy to bacterial photoinactivation. Biochem Mol Biol Educ 2005; 33: 46-9
    CrossrefPubMedGoogle Scholar
  • 23 Lee YH, Park HW, Lee JH, Seo HW, Lee SY. The photodynamic therapy on Streptococcus mutans biofilms using erythrosine and dental halogen curing unit. Int J Oral Sci 2012; 4: 196-201
    CrossrefPubMedGoogle Scholar
  • 24 Araújo NC, Fontana CR, Bagnato VS, Gerbi ME. Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine. Lasers Med Sci 2014; 29: 629-35
    CrossrefPubMedGoogle Scholar
  • 25 Vahabi S, Fekrazad R, Ayremlou S, Taheri S, Zangeneh N. The effect of antimicrobial photodynamic therapy with radachlorin and toluidine blue on Streptococcus mutans: An in vitro study. J Dent (Tehran) 2011; 8: 48-54
    Google Scholar
  • 26 Nogueira AC, Graciano AX, Nagata JY, Fujimaki M, Terada RS, Bento AC. et al. Photosensitizer and light diffusion through dentin in photodynamic therapy. J Biomed Opt 2013; 18: 55004
    CrossrefPubMedGoogle Scholar
  • 27 Gursoy H, Ozcakir-Tomruk C, Tanalp J, Yilmaz S. Photodynamic therapy in dentistry: A literature review. Clin Oral Investig 2013; 17: 1113-25
    CrossrefPubMedGoogle Scholar
  • 28 Pereira CA, Costa AC, Carreira CM, Junqueira JC, Jorge AO. Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro . Lasers Med Sci 2013; 28: 859-64
    CrossrefPubMedGoogle Scholar
  • 29 Rolim JP, de-Melo MA, Guedes SF, Albuquerque-Filho FB, de Souza JR, Nogueira NA. et al. The antimicrobial activity of photodynamic therapy against Streptococcus mutans using different photosensitizers. J Photochem Photobiol B 2012; 106: 40-6
    CrossrefPubMedGoogle Scholar
  • 30 Garcez AS, Núñez SC, Azambuja Jr N, Fregnani ER, Rodriguez HM, Hamblin MR. et al. Effects of photodynamic therapy on Gram-positive and Gram-negative bacterial biofilms by bioluminescence imaging and scanning electron microscopic analysis. Photomed Laser Surg 2013; 31: 519-25
    CrossrefPubMedGoogle Scholar
  • 31 Longo JP, Azevedo RB. Effect of photodynamic therapy mediated by methylene blue in cariogenic bacteria. J Dent Clin Res 2010; 6: 249-57
    Google Scholar
  • 32 Bowden GH, Hamilton IR. Competition between Streptococcus mutans and Lactobacillus casei in mixed continuous culture. Oral Microbiol Immunol 1989; 4: 57-64
    CrossrefPubMedGoogle Scholar
  • 33 Wilson M. Susceptibility of oral bacterial biofilms to antimicrobial agents. J Med Microbiol 1996; 44: 79-87
    CrossrefPubMedGoogle Scholar