This study was conducted to investigate the susceptibility of the biofilm cells of Escherichla coil O 157, Salmonella Enteritidis, and Staphylococcus aureus to some cleaning detergents and sanitizers. No weakly acidic, neutral, and weakly alkaline detergent could remove the biofilm bacteria from stainless steel chips at commonly used concentrations recommended by manufacturers. Among sanitizers, sodium hypochlorite did not completely inactivate any biofilm bacteria at active chlorine concentrations of 25 to 200μg/ml. Benzalkonium chloride, alkyldiaminoethyl glycine hydrochloride, chlorhexidine digluconate, and polyhexamethylenebiganide inactivated the great majority of E. coil and S. Enteritidis at commonly used concentrations, but did not inactivate S. aureus effectively enough. The biofilm S. aureus population was shown to be more tolerant than the E. coil and S. Enteritidis populations to the sanitizers.