EFFECTIVENESS OF INTRAGASTRIC IMMUNIZATION WITH PROTEIN AND OLIGODEOXYNUCLEOTIDES CONTAINING A CpG MOTIF FOR INDUCING A GASTROINTESTINAL MUCOSAL IMMUNE RESPONSE IN MICE

TAKUTO HIKICHI; HIROKO KOBAYASHI; HITOSHI OYAMA; GO YAMAMOTO; HIROSHI WATANABE; ATSUSHI IRISAWA; KATSUTOSHI OBARA; YUKIO SATO

doi:10.5387/fms.51.19

Original Article

EFFECTIVENESS OF INTRAGASTRIC IMMUNIZATION WITH PROTEIN AND OLIGODEOXYNUCLEOTIDES CONTAINING A CpG MOTIF FOR INDUCING A GASTROINTESTINAL MUCOSAL IMMUNE RESPONSE IN MICE

TAKUTO HIKICHI, HIROKO KOBAYASHI, HITOSHI OYAMA, GO YAMAMOTO, HIROSHI WATANABE, ATSUSHI IRISAWA, KATSUTOSHI OBARA, YUKIO SATO

Author information

TAKUTO HIKICHI
Department of Internal Medicine II, Fukushima Medical University School of Medicine
HIROKO KOBAYASHI
Department of Internal Medicine II, Fukushima Medical University School of Medicine
HITOSHI OYAMA
Department of Internal Medicine II, Fukushima Medical University School of Medicine
GO YAMAMOTO
Department of Internal Medicine II, Fukushima Medical University School of Medicine
HIROSHI WATANABE
Department of Internal Medicine II, Fukushima Medical University School of Medicine
ATSUSHI IRISAWA
Department of Internal Medicine II, Fukushima Medical University School of Medicine
KATSUTOSHI OBARA
Department of Internal Medicine II, Fukushima Medical University School of Medicine
YUKIO SATO
Department of Internal Medicine II, Fukushima Medical University School of Medicine

Keywords: mucosal vaccine, mucosal immune response, intragastric immunization, CpG motif, Helicobacter pylori

JOURNAL OPEN ACCESS

2005 Volume 51 Issue 1 Pages 19-31

DOI https://doi.org/10.5387/fms.51.19

Details

Abstract

[Purpose] To investigate a new modality of mucosal vaccines, we evaluated the effectiveness of intragastric immunization for inducing a mucosal immune response in the gastrointestinal tract. [Methods] Mice were immunized with β-galactosidase (β-gal) and synthesized oligodeoxynucleotides containing a CpG motif (CpG-DNA) by intragastric injection, and the immune response was compared with those induced by 3 other immunization forms: intranasal, oral, and intradermal. [Results] Intragastric immunization with β-gal and CpG-DNA induced significant anti-β-gal fecal IgA production at 2 weeks; however, at 4 weeks the response was lacking. In contrast, intranasal immunization with β-gal and CpG-DNA induced the highest anti-β-gal fecal IgA production at 4 weeks. [Conclusion] Although intragastric immunization with protein and CpG-DNA induces a mucosal immune response in the gastrointestinal tract, intranasal immunization is the most effective to induce both mucosal and systemic immune responses. This finding may increase the possibility for developing vaccines against mucosal pathogens, especially Helicobacter pylori.

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