Biomed. Papers 145(2), 49-55 (2001) | DOI: 10.5507/bp.2001.011

NEONATAL RAT CARDIOMYOCYTES – A MODEL FOR THE STUDY OF MORPHOLOGICAL, BIOCHEMICAL AND ELECTROPHYSIOLOGICAL CHARACTERISTICS OF THE HEART

©árka Chlopčíková, Jitka Psotová, Petra Miketová
Institute of Medical Chemistry and Biochemistry, Medical Faculty of Palacký University, 775 15 Olomouc, Czech Republic

Dedicated to the 60th birthdays of prof. MUDr. Václav Lichnovský, DrSc.The neonatal rat cardiomyocyte model enables heart researchers to study and understand the morphological,biochemical and electrophysiological characteristics of the heart. This model offers a broad spectrum of experiments,such as studies of contraction, ischaemia, hypoxia and the toxicity of various compounds. This reviewexamines the methodology for the isolation and cultivation of primary neonatal rat cardiomyocyte cultures,considers possible problems and pitfalls, and presents an optimized protocol for cardiomyocyte preparation.

Keywords: Rat cardiomyocytes, Cell culture, Neonatal rat

Received: September 10, 2001; Published: December 1, 2001  Show citation

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Chlopčíková, ©., Psotová, J., & Miketová, P. (2001). NEONATAL RAT CARDIOMYOCYTES – A MODEL FOR THE STUDY OF MORPHOLOGICAL, BIOCHEMICAL AND ELECTROPHYSIOLOGICAL CHARACTERISTICS OF THE HEART. Biomedical papers, 145(2), 49-55. doi: 10.5507/bp.2001.011
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