LASER THERAPY
Online ISSN : 1884-7269
Print ISSN : 0898-5901
ISSN-L : 0898-5901
ORIGINAL ARTICLES
THE DIRECT EFFECT OF 860 nm LIGHT ON CELL PROLIFERATION AND ON SUCCINIC DEHYDROGENASE ACTIVITY OF HUMAN FIBROBLASTS INVITRO
Peter BoltonSteve YoungMary Dyson
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JOURNAL FREE ACCESS

1995 Volume 7 Issue 2 Pages 55-60

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Abstract

Human forearm fibroblasts were irradiated, in vitro, using a Microlaser Medical Pen Unit (Johnson and Johnson) with a wavelength of 860 nm, and an average power output of 50 mW. The irradiation distance and times were adjusted to give energy densities of 2 J/cm2 and 16 J/cm2 respectively. The control unit was adjusted to give a continuous waveform. Fibroblast proliferation and succinic dehydrogenase activity were assessed every 24 hrs over a five day period. The results obtained suggest a relationship between fibroblast proliferation and succinic dehydrogenase activity. At the lower energy density (2 J/cm2), succinic dehydrogenase levels rise with fibroblast proliferation. However, at an energy density of 16 J/cm2, the succinic dehydrogenase levels and fibroblast proliferation appear to be inhibited. These findings agree with previously reported data.

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© 1995 Japan Medical Laser Laboratory
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