Sulfobacins A and B, Novel von Willebrand Factor Receptor Antagonists I. Production, Isolation, Characterization and Biological Activities

TSUTOMU KAMIYAMA; TAKAYUKI UMINO; TOMOKO SATOH; SAYOKO SAWAIRI; MICHIKO SHIRANE; SHOICHI ONSHIMA; KAZUTERU YOKOSE

doi:10.7164/antibiotics.48.924

Sulfobacins A and B, Novel von Willebrand Factor Receptor Antagonists

I. Production, Isolation, Characterization and Biological Activities

TSUTOMU KAMIYAMA, TAKAYUKI UMINO, TOMOKO SATOH, SAYOKO SAWAIRI, MICHIKO SHIRANE, SHOICHI ONSHIMA, KAZUTERU YOKOSE

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JOURNAL FREE ACCESS

1995 Volume 48 Issue 9 Pages 924-928

DOI https://doi.org/10.7164/antibiotics.48.924

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Abstract

Sulfobacins A and B, novel von Willebrand factor (vWF) receptor antagonists, have been isolated from the culture broth of Chryseobacterium sp. (Flavobacterium sp.) NR 2993 by ethyl acetate extraction, and by Sephadex LH-20 and silica gel column chromatographies. The physico-chemical properties of the Sulfobacins indicate that their structures are completely different from that of aurintricarboxylic acid, the one known vWF receptor antagonist. Sulfobacins A and B inhibit the binding of vWF.to its receptor with IC₅₀s of 0.47 and 2.2/IM, respectively. Sulfobacin A also inhibits ristocetin-induced agglutination in human platelets fixed with paraformaldehyde with an IC₅₀ of 0.58 μM.

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