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Archives of Gynecology and Obstetrics

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04.05.2018 | Gynecologic Oncology

Liquid-based cytology: do ancillary techniques enhance detection of epithelial abnormalities?

verfasst von: Sridhar Mishra, Nuzhat Husain, Namrata P. Awasthi, Yashodhara Pradeep, Roohi Roohi, Sarita Saxena

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 1/2018

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Abstract

Purpose

Cervical cancer is the fourth most common cancer in women worldwide with very high incidence in India. Liquid-based cytology (LBC) provides the use of ancillary techniques in addition to a good morphology and detection of cytologic abnormalities. The current study was designed to assess the diagnostics of P16INK4a immunoexpression, p16 promoter hypermethylation, human papilloma virus (HPV), and DNA ploidy in LBC samples with cervical precancer and cancer.

Methods

A series of LBC samples categorised by Bethesda system including 22 atypical squamous cells of undetermined significance (ASC-US), 21 low-grade squamous intraepithelial lesion (LSIL), 41 high-grade squamous intraepithelial lesion (HSIL), 54 squamous cell carcinoma (SCC), and 26 controls with normal cytology were included. Ancillary techniques evaluated included P16INK4a immunoexpression, p16 promoter methylation DNA ploidy by flow cytometry, and HPV was detected using PGMY09/PGMY11 primers.

Results

The test positivity rate of p16 expression in women with ASC-US, LSIL, HSIL, and SCC was 21.1, 39.0, 67.7, and 85.4%. For the p16 methylation the corresponding test positivity rate was 36.4, 76.2, 92.7, and 92.6%. The test positive rate of HPV in women with ASC-US, LSIL, HSIL, and SCC was 45.5, 76.2, 87.8, and 92.6%. Diploid G1 and diploid S values significantly (p < 0.05 or p < 0.01) discriminate LSIL versus HSIL and LSIL versus. SCC.

Conclusions

P16 gene promoter methylation and HPV seem more sensitive in detection of ASC-US and LSIL cytology with higher specificity. Diploid G1 and diploid S phase study provides progressive change in parameters with progression from LSIL to HSIL and SCC.

World Health Organization (WHO). Human papillomavirus infection and cervical cancer. www.who.int/vaccine_research/diseases/hpv

Cuschieri KS, Beattie G, Hassan S, Robertson K, Cubie HA (2005) Assessment of human papillomavirus mRNA detection over time in cervical specimens collected in liquid-based cytology medium. J Virol Methods 124:211–215CrossRef

Dimulescu II, Unger ER, Lee DR, Reeves WC, Vernon SD (1998) Characterization of RNA in cytologic samples preserved in a methanol-based collection solution. Mol Diagn 3:67–71CrossRef

Esteller M, Herman JG (2002) Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumours. J Pathol. 196:1–7CrossRef

Melsheimer P, Klaes R, von Knebel Doeberitz M, Bastert G (2001) Prospective clinical study comparing DNA flow cytometry and HPV typing as predictive tests for persistence and progression of CIN I/II. Clin Cytom 46(3):166–171CrossRef

Kashyap V, Das DK, Luthra UK (1990) Micro photometric nuclear DNA analysis in cervical dysplasia of the uterine cervix: its relation to the progression to malignancy and regression to normalcy. Neoplasma 37(5):497–500PubMed

Melsheimer P, Vinokurova S, Wentzensen N, Bastert G, von Knebel DM (2004) DNA aneuploidy and integration of human papillomavirus type 16 E6/E7 oncogenes in intraepithelial neoplasia and invasive squamous cell carcinoma of the cervix uteri. Clin Cancer Res 10(9):3059–3063CrossRef

Winkler B, Crum CP, Fujii T et al (1984) Koilocytotic lesions of the cervix. The relationship of mitotic abnormalities to the presence of papillomavirus antigens and nuclear DNA content. Cancer 53(5):1081–1087CrossRef

Singh M, Mehrotra S, Kalra N, Singh U, Shukla Y (2008) Correlation of DNA ploidy with progression of cervical cancer. J Cancer Epidemiol. https://doi.org/10.1155/2008/298495 CrossRefPubMedPubMedCentral

10.

Agoff N, Lin P, Morihara J, Mao C, Kiviat N, Koutsky L (2003) p16INK4a expression correlates with degree of cervical neoplasia: a comparison with Ki-67 expression and detection of high-risk HPV types. Mod Pathol 16:665–673CrossRef

11.

Stanley MA (2002) Prognostic factors and new therapeutic approaches to cervical cancer. Virus Res 89:241–248CrossRef

12.

Baylin SB, Esteller M, Rountree MR, Bachman KE, Schuebel K, Herman JG (2001) Aberrant patterns of DNA methylation, chromatin formation and gene expression in cancer. Hum Mol Genet 10:687–692CrossRef

13.

Mishra S, Awasthi NP, Husain N, Anand A, Pradeep Y, Roohi Saxena S (2017) Flow cytometric analysis of DNA ploidy in liquid based cytology for cervical pre-cancer and cancer. Asian Pac J Cancer Prev 18(6):1595–1601PubMedPubMedCentral

14.

Gravitt PE, Kamath AM, Gaffikin L, Chirenje ZM, Womack S, Shah KV (2002) Human papillomavirus genotype prevalence in high-grade squamous intraepithelial lesions and colposcopically normal women from Zimbabwe. Int J Cancer 100(6):729–732CrossRef

15.

Sørbye SW, Pedersen MK, Ekeberg B, Williams MEJ, Sauer T, Chen Y (2017) Can an inadequate cervical cytology sample in ThinPrep be converted to a satisfactory sample by processing it with a SurePath preparation? Cytojournal 22(14):20CrossRef

16.

Klaes R, Benner A, Friedrich T et al (2002) p16INK4a immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. Am J Surg Pathol 26(11):1389–1399CrossRef

17.

Arbyn M, Ronco G, Cuzick J, Wentzensen N, Castle PE (2009) How to evaluate emerging technologies in cervical cancer screening? Int J Cancer 125(11):2489–2496CrossRef

18.

Sawaya GF, Grimes DA (1999) New technologies in cervical cytology screening: a word of caution. Obstet Gynaecol 94(2):307–310

19.

Bulkmans NW, Bleeker MC, Berkhof J, Voorhorst FJ, Snijders PJ, Meijer CJ (2005) Prevalence of types 16 and 33 is increased in high-risk human papillomavirus positive women with cervical intraepithelial neoplasia grade 2 or worse. Int J Cancer 117(2):177–181CrossRef

20.

Arbyn M, Sankaranarayanan R, Muwonge R et al (2008) Pooled analysis of the accuracy of five cervical cancer screening tests assessed in eleven studies in Africa and India. Int J Cancer 123(1):153–160CrossRef

21.

Sulik SM, Kroeger K, Jennifer K, Jennie LS, Lorne AB, Grant WDB (2001) Are fluid-based cytologies superior to the conventional Papanicolaou test? A systematic review. J Fam Pract 50:1040–1046PubMed

22.

Bernstein SJ, Sanchez-Ramos L, Ndubisi B (2001) Liquid-based cervical cytologic smear study and conventional Papanicolaou smears: a metaanalysis of prospective studies comparing cytologic diagnosis and sample adequacy. Am J Obstet Gynecol 185:308–317CrossRef

23.

Koss LG (1989) The Papnicolaou test for cervical cancer detection: a triumph and a tragedy. J Am Med Assoc 261:737–743CrossRef

24.

Zahniser D, Sullivan PJ (1996) Cytyc corporation. Acta Cytol 40:37–44CrossRef

25.

Rozemeijer K, Penning C, Siebers AG, Naber SK, Matthijsse SM, van Ballegooijen M, van Kemenade FJ, de Kok IM (2016) Comparing SurePath, ThinPrep, and conventional cytology as primary test method: SurePath is associated with increased CIN II+ detection rates. Cancer Causes Control 27(1):15–25CrossRef

26.

Rozemeijer K, Naber SK, Penning C, Overbeek LI, Looman CW, de Kok IM, Matthijsse SM, Rebolj M, van Kemenade FJ, van Ballegooijen M (2017) Cervical cancer incidence after normal cytological sample in routine screening using SurePath, ThinPrep, and conventional cytology: population based study. BMJ 356:j504. https://doi.org/10.1136/bmj.j504 CrossRefPubMedPubMedCentral

27.

Rebolj M, Rask J, van Ballegooijen M, Kirschner B, Rozemeijer K, Bonde J, Rygaard C, Lynge E (2015) Cervical histology after routine ThinPrep or SurePath liquid-based cytology and computer-assisted reading in Denmark. Br J Cancer 113(9):1259–1274CrossRef

28.

Bosch FX, Manos MM, Munoz N et al (1995) Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer (IBSCC) Study Group. J Natl Cancer Inst 87(11):796–802CrossRef

29.

Clifford GM, Smith JS, Plummer M, Munoz N, Franceschi S (2003) Human papillomavirus types in invasive cervical cancer worldwide: a meta-analysis. Br J Cancer 88(1):63–73CrossRef

30.

Virmani AK, Muller C, Rathi A, Zoechbauer-Mueller S, Mathis M, Gazdar AF (2001) Aberrant methylation during cervical carcinogenesis. Clinical Cancer Res 7:584–589

31.

Hirama T, Koeffer HP (1995) Role of the cyclin dependent kinase inhibitors in the development of cancer. Blood 86:841–854PubMed

32.

Nuovo GJ, Plaia TW, Belinsky SA, Baylin SB, Herman JG (1999) In situ detection of the hypermethylation-induced inactivation of the p16 gene as an early event in oncogenesis. Proc Natl Acad Sci USA 96(22):12754–12759CrossRef

33.

Huang T, Chen X, Hong Q et al (2015) Meta-analyses of gene methylation and smoking behaviour in non-small cell lung cancer patients. Sci Rep 5:88–97

34.

Yuan-ying MA, Xiao-dong C, Cai-yun Z et al (2011) Value of p16 expression in the triage of liquid-based cervical cytology with atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesions. Chin Med J 24(16):2443–2447

35.

Wright TC Jr, Massad LS, Dunton CJ, Spitzer M, Wilkinson EJ, Solomon D (2006) Consensus guidelines for the management of women with abnormal cervical screening tests. J Low Genit Tract Dis 11:201–222CrossRef

36.

Mao C, Balasubramanian A, Yu M et al (2007) Evaluation of a new p16INK4A ELISA test and a high-risk HPV DNA test for cervical cancer screening: results from proof-of-concept study. Int J Cancer 120:2435–2438CrossRef

37.

Wentzensen N, Schwartz L, Zuna RE et al (2012) Performance of p16/Ki-67 immunostaining to detect cervical cancer precursors in a colposcopy referral population. Clin Cancer Res 18(15):4154–4162CrossRef

38.

Gustinucci D, Giorgi Rossi P, Cesarini E et al (2016) Use of cytology, E6/E7 mRNA, and p16INK4a-Ki-67 to define the management of human papillomavirus (HPV)-positive women in cervical cancer screening. Am J Clin Pathol 145(1):35–45CrossRef

39.

Saxena M, Negi MP, Singh S, Singh PK, Singh U, Bhatt ML (2010) DNA content can improve the detection and prognosis of carcinoma of the cervix. Bio Sci Trends 4:103–109

40.

Jayat C, Ratinaud MH (1993) Cell cycle analysis by flow cytometry: principal and application. Biol Cell 78:15–25CrossRef

41.

Lai CH, Hsueh S, Huang MY, Chang MF, Soong YK (1993) The uses and limitations of DNA flow cytometry in stage IB or II cervical carcinoma. Cancer 72:3655–3662CrossRef

Titel: Liquid-based cytology: do ancillary techniques enhance detection of epithelial abnormalities?
verfasst von: Sridhar Mishra
Nuzhat Husain
Namrata P. Awasthi
Yashodhara Pradeep
Roohi Roohi
Sarita Saxena
Publikationsdatum: 04.05.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 1/2018
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-018-4763-z

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