Background
Methods
Patients and tissue samples
Cell culture
Plasmids and cell transfection
Reverse transcription and quantitative real-time PCR
Western blot analysis
Cell proliferation assays
Transwell assays
In situ hybridization assay and immunohistochemistry analysis
Dual luciferase reporter assay
RNA immunoprecipitation (RIP) assay
In vivo nude mouse models
Statistical analysis
Results
DANCR was elevated in osteosarcoma tissues and cell lines and was correlated with poor prognosis in osteosarcoma patients
Features | No. of cases | DANCR | p value† | |
---|---|---|---|---|
High | Low | |||
Age at diagnosis | 0.481 | |||
<18 | 52 | 40 | 12 | |
≥18 | 43 | 32 | 11 | |
Gender | 0.472 | |||
Female | 51 | 38 | 13 | |
Male | 44 | 34 | 10 | |
Histological subtype | 0.674 | |||
Osteoblastic | 9 | 6 | 3 | |
Chondroblastic | 18 | 12 | 6 | |
Fibroblastic | 38 | 30 | 8 | |
Mixed | 30 | 24 | 6 | |
Clinical stage | 0.005 | |||
I + IIA | 42 | 26 | 16 | |
IIB/III | 53 | 46 | 7 | |
Distant metastasis | 0.002 | |||
Absent | 37 | 22 | 15 | |
Present | 58 | 50 | 8 | |
Tumor size (cm) | 0.014 | |||
<5 | 39 | 23 | 16 | |
≥ 5 | 56 | 49 | 7 | |
Anatomic location | 0.431 | |||
Tibia/femur | 49 | 38 | 11 | |
Elsewhere | 46 | 34 | 12 |