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01.06.2010 | Original Article

Long-term and short-term effects of simulated birth trauma, cesarean and vaginal delivery on sulfated glycosaminoglycans in the urethra of female rats

verfasst von: Claudia Cristina Takano, Leonardo Robson Pinheiro Sobreira Bezerra, Eliana Viana Monteiro Zucchi, Helena Bonciani Nader, Marair Gracio Ferreira Sartori, Manoel João Batista Castelo Girão

Erschienen in: International Urogynecology Journal | Ausgabe 6/2010

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Abstract

Introduction and hypothesis

This study assesses the effects of simulated birth trauma, vaginal and cesarean delivery on glycosaminoglycans in the connective tissue of female rat urethra.

Methods

One hundred and ten female rats were divided into six groups: A—control, B—vaginal trauma, C—cesarean delivery, D—cesarean delivery followed by vaginal trauma, E—vaginal delivery, and F—female rats on the 20th day of gestation. In each group, half of the animals were sacrificed 4 days after the procedures and half were sacrificed 12 weeks later. Glycosaminoglycans were extracted by proteolysis and analyzed using densitometry after agarose gel electrophoresis.

Results

The examination at 4 days showed larger amounts of glycosaminoglycans in pregnant female rats compared to other groups. The examination at 12 weeks showed larger amounts of glycosaminoglycans in the cesarean delivery group followed by simulated birth trauma.

Conclusions

There is a change in sulfated glycosaminoglycans in female rat urethra during pregnancy and simulated birth trauma.

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Titel: Long-term and short-term effects of simulated birth trauma, cesarean and vaginal delivery on sulfated glycosaminoglycans in the urethra of female rats
verfasst von: Claudia Cristina Takano
Leonardo Robson Pinheiro Sobreira Bezerra
Eliana Viana Monteiro Zucchi
Helena Bonciani Nader
Marair Gracio Ferreira Sartori
Manoel João Batista Castelo Girão
Publikationsdatum: 01.06.2010
Verlag: Springer-Verlag
Erschienen in: International Urogynecology Journal / Ausgabe 6/2010
Print ISSN: 0937-3462
Elektronische ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-009-1087-3

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Springer Medizin

Long-term and short-term effects of simulated birth trauma, cesarean and vaginal delivery on sulfated glycosaminoglycans in the urethra of female rats

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

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Springer Medizin

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

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