The online version of this article (https://doi.org/10.1186/s12885-018-4022-5) contains supplementary material, which is available to authorized users.
Metastatic breast cancer (BCa) is most often diagnosed months after completion of treatment of the primary tumor when a patient reports physical symptoms. Besides a physical examination, no other alternative recurrence screening method is recommended for routine follow-up care. Detection of autoantibodies against tumor-associated antigens (TAAs) has demonstrated promise for distinguishing healthy women from patients diagnosed with primary BCa. However, it is unknown what changes occur to patient autoantibody levels during and after treatment.
Three serial blood draws were collected from 200 BCa patients: before treatment, 6 and 12 months after surgery. Patients were categorized according to treatment regimen, including surgery, chemotherapy, radiation, trastuzumab and hormonal therapies. The longitudinal samples were assayed for autoantibody responses against 32 conformation-carrying TAAs using a Luminex multiplex bead assay.
The treatment modality groups that had the greatest decrease in autoantibody response levels were radiation + hormonal therapy; radiation + chemotherapy; and radiation + hormonal therapy + chemotherapy. For these three treatment groups, autoantibody responses against 9 TAAs (A1AT, ANGPTL4, CAPC, CST2, DKK1, GFRA1, GRN, LGALS3 and LRP10) were significantly reduced at 12 months after surgery compared to before treatment. One TAA, GRP78, had a significantly increased autoantibody response after 12 months.
Single treatment regimens alone did not significantly alter autoantibodies levels against the studied TAAs. Radiation treatment was the common denominator of the three most affected groups for significant changes in autoantibody response levels.
Additional file 1: Table indicating inter-assay coefficients of variability (CV) for the Luminex multiplex immunoassay. Shown are the average inter-assay CV for the autoantibody responses against the 32 TAAs for negative and positive controls. Calculations were measured at baseline (before the start of treatment) using the Luminex multiplex bead platform. (DOCX 32 kb)12885_2018_4022_MOESM1_ESM.docx
Additional file 2: Observed geometric mean changes of patients’ autoantibody responses at 6 months after the start of treatment. The graph indicates the observed geometric mean changes (with 95% confidence intervals) of autoantibody levels against 11 tumor-associated antigens according to treatment regimen after 6 months follow-up. *indicates p-value < 0.05. There were no significant changes observed for surgery only or individual therapies (i.e. hormonal, radiation, or chemotherapy). (DOCX 117 kb)12885_2018_4022_MOESM2_ESM.docx
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- Longitudinal autoantibody responses against tumor-associated antigens decrease in breast cancer patients according to treatment modality
Rick L. Evans
James V. Pottala
Kristi A. Egland
- BioMed Central
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