Background
Methods
Patient information and tissue specimens
Immunohistochemistry
Staining evaluation
Cell lines and reagents
Immunofluorescence assay
Gene infection
Western blot
Cell proliferation assay
Plate colony formation assay
DNA replication activity assay
Xenograft study in nude mice
Statistical analysis
Results
Association between decreased expression of NDRG2 and progression of ESCC
Variable | Number of cases (%) | Variable | Number of cases (%) |
---|---|---|---|
Gender | Cigarette smoking | ||
Male | 95 (66.4) | No | 51 (35.7) |
Female | 48 (33.6) | Yes | 92 (64.3) |
Age(years) | Alcohol drinking | ||
≤60 | 78 (54.5) | No | 37 (25.9) |
>60 | 65 (45.5) | Yes | 106 (74.1) |
Clinical stage | T classification | ||
I | 5 (3.5) | T1 | 9 (6.3) |
IIA | 64 (44.8) | T2 | 52 (36.4) |
IIB | 23 (16.1) | T3 | 63 (44.1) |
III | 38 (26.6) | T4 | 19 (13.2) |
IV | 13 (9.0) | Histological differentiation | |
N classification | Well | 45 (31.4) | |
N0 | 61 (42.7) | Moderate | 50 (35.0) |
N1 | 69 (48.3) | Poor | 48 (33.6) |
N2 | 13 (9.0) | Expression of NDRG2 | |
M classification | Negative | 83 (58.0) | |
M0 | 130 (90.9) | Positive | 60 (42.0) |
M1 | 13 (9.1) | Location | |
Therapy | Upper | 17 (11.9) | |
Surgery only | 80 (55.9) | Middle | 80 (55.9) |
Surge + CT/RT/CRT | 63 (44.1) | Lower | 46 (32.2) |
Complication | |||
Yes | 35 (24.5) | ||
No | 108 (75.5) |
Variable | NDRG2 expression |
p
| |
---|---|---|---|
Negative (%) | Positive (%) | ||
Gender | |||
Male | 57 (60.0) | 38 (40.0) | 0.154a
|
Female | 26 (54.2) | 22 (45.8) | |
Age(years) | |||
≤60 | 50 (64.1) | 28 (35.9) | 0.127 a
|
>60 | 33 (50.8) | 32 (49.2) | |
Cigarette smoking | |||
No | 33 (64.7) | 18 (35.3) | 0.289 a
|
Yes | 50 (54.3) | 42 (45.7) | |
Alcohol drinking | |||
No | 17 (45.9) | 20 (54.1) | 0.121 a
|
Yes | 66 (62.3) | 40 (37.7) | |
Clinical stage | |||
I | 1 (20.0) | 4 (80.0) | 0.009b
|
IIA | 29 (45.3) | 35 (54.7) | |
IIB | 15 (65.2) | 8 (34.8) | |
III | 28 (73.7) | 10 (26.3) | |
IV | 10 (76.9) | 3 (23.1) | |
T classification | |||
T1 | 1 (11.1) | 8 (88.9) | <0.0001b
|
T2 | 23 (44.2) | 29 (55.8) | |
T3 | 43 (68.3) | 20 (31.7) | |
T4 | 16 (84.2) | 3 (15.8) | |
N classification | |||
N0 | 22 (36.1) | 39 (63.9) | <0.0001b
|
N1 | 50 (72.5) | 19 (27.5) | |
N2 | 11 (84.6) | 2 (15.4) | |
M classification | |||
M0 | 72 (55.4) | 58 (44.6) | 0.036a
|
M1 | 11 (84.6) | 2 (15.4) | |
Histological differentiation | |||
Well | 18 (40.0) | 27 (60.0) | 0.005b
|
Moderate | 30 (60.0) | 20 (40.0) | |
Poor | 35 (72.9) | 13 (27.1) |
Variable | NDRG2 expression |
p
a
|
---|---|---|
Correlation coefficient (r
s
)
| ||
Gender | -.121 | .124 |
Age(years) | -.135 | .109 |
Cigarette smoking | .101 | .232 |
Alcohol drinking | -.145 | .084 |
Clinical stage | -.299 | .0001 |
T classification | -.347 | .0001 |
N classification | -.387 | .0001 |
M classification | -.170 | .042 |
Histological differentiation | -.268 | .001 |