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30.03.2018 | Original Article | Ausgabe 5/2018

Lasers in Medical Science 5/2018

Low-level laser irradiation at a high power intensity increased human endothelial cell exosome secretion via Wnt signaling

Lasers in Medical Science > Ausgabe 5/2018
Hesam Saghaei Bagheri, Monireh Mousavi, Aysa Rezabakhsh, Jafar Rezaie, Seyed Hossein Rasta, Alireza Nourazarian, Çigir Biray Avci, Habib Tajalli, Mehdi Talebi, Ahmad Oryan, Majid Khaksar, Masoumeh Kazemi, Seyed Mahdi Nassiri, Shahrooz Ghaderi, Bakiye Goker Bagca, Reza Rahbarghazi, Emel Sokullu
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Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1007/​s10103-018-2495-8) contains supplementary material, which is available to authorized users.
Reza Rahbarghazi and Emel Sokullu contributed equally to this work.
Hesam Saghaei Bagheri, Monireh Mousavi, and Alireza Nourazarian are considered equally as co-first authors.
A correction to this article is available online at https://​doi.​org/​10.​1007/​s10103-019-02921-2.


The distinct role of low-level laser irradiation (LLLI) on endothelial exosome biogenesis remains unclear. We hypothesize that laser irradiation of high dose in human endothelial cells (ECs) contributes to the modulation of exosome biogenesis via Wnt signaling pathway. When human ECs were treated with LLLI at a power density of 80 J/cm2, the survival rate reduced. The potential of irradiated cells to release exosomes was increased significantly by expressing genes CD63, Alix, Rab27a, and b. This occurrence coincided with an enhanced acetylcholine esterase activity, pseudopodia formation, and reduced zeta potential value 24 h post-irradiation. Western blotting showed the induction of LC3 and reduced level of P62, confirming autophagy response. Flow cytometry and electron microscopy analyses revealed the health status of the mitochondrial function indicated by normal ΔΨ activity without any changes in the transcription level of PINK1 and Optineurin. When cells exposed to high power laser irradiation, p-Akt/Akt ratio and in vitro tubulogenesis capacity were blunted. PCR array and bioinformatics analyses showed the induction of transcription factors promoting Wnt signaling pathways and GTPase activity. Thus, LLLI at high power intensity increased exosome biogenesis by the induction of autophagy and Wnt signaling. LLLI at high power intensity increases exosome biogenesis by engaging the transcription factors related to Wnt signaling and autophagy stimulate.

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