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Erschienen in: HSS Journal ® 2/2016

10.03.2016 | Original Article

Low Levels of Vitamin D have a Deleterious Effect on the Articular Cartilage in a Rat Model

verfasst von: Cecilia Pascual-Garrido, MD, Michael E. Angeline, MD, Richard Ma, MD, Jorge Chahla, MD, Cliff Voigt, MD, Xiang Hua Deng, MD, Joseph Nguyen, MPH, Russell F. Warren, MD, Scott A. Rodeo, MD

Erschienen in: HSS Journal ® | Ausgabe 2/2016

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Abstract

Background

Vitamin D appears to play an important role in bone and cartilage metabolism since its receptors are widely found in human articular chondrocytes. Thus, effects of variation of vitamin D may directly impact cartilage and bone biology.

Questions/Purposes

The aims of this study are to compare (1) articular cartilage structure and composition and (2) trabecular and cortical bone microstructure in rats with normal versus insufficient vitamin D levels.

Methods

Twenty-five mature, male Sprague-Dawley rats were allocated to two groups: (1) control arm (vitamin D replete—12 rats) and (2) an experimental arm (vitamin D deficient—13 rats). Vitamin D deficiency was induced using a vitamin D-deficient diet and UV light restriction. Rats were sacrificed after 4 weeks vitamin D deficiency was confirmed. The right knee was harvested for analysis of both the medial (MFC) and lateral femoral condyles (LFC). A region of interest was established on both condyles to correlate subchondral bone architecture and the overlying cartilage. Histological analysis was performed and graded using the modified Mankin score. Subchondral and cortical bony architecture was evaluated with micro-CT.

Results

After 4 weeks, the vitamin D-deficient group had statistically significant changes in cartilage structure in both the MFC and LFC [1.55 ± 0.6 vs. 4.23 ± 4.1 (p = 0.035) and 1.55 ± 0.6 vs. 3.53 ± 2.4 (p = 0.009), respectively]. Micro-CT analysis revealed no correlation between subchondral bone values and the overlying cartilage Mankin score (p = 0.460). No significant difference was evident between the subchondral bone of the control and study group.

Conclusions

Low levels of vitamin D have a deleterious effect on the cartilage.
Given the high prevalence of vitamin D deficiency in the general population, these findings raise important questions about the potential role of vitamin D in articular cartilage health.
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Literatur
1.
Zurück zum Zitat Bahar-Shany K, Ravid A, Koren R. Upregulation of MMP-9 production by TNFalpha in keratinocytes and its attenuation by vitamin D. J Cell Physiol. 2010; 222(3): 729-737.PubMed Bahar-Shany K, Ravid A, Koren R. Upregulation of MMP-9 production by TNFalpha in keratinocytes and its attenuation by vitamin D. J Cell Physiol. 2010; 222(3): 729-737.PubMed
2.
Zurück zum Zitat Bergink AP, Uitterlinden AG, Van Leeuwen JP, et al. Vitamin D status, bone mineral density, and the development of radiographic osteoarthritis of the knee: The Rotterdam Study. J Clin Rheumatol. 2009; 15(5): 230-237.CrossRefPubMed Bergink AP, Uitterlinden AG, Van Leeuwen JP, et al. Vitamin D status, bone mineral density, and the development of radiographic osteoarthritis of the knee: The Rotterdam Study. J Clin Rheumatol. 2009; 15(5): 230-237.CrossRefPubMed
4.
Zurück zum Zitat Bolland MJ, Grey A, Avenell A, Gamble GD, Reid IR. Calcium supplements with or without vitamin D and risk of cardiovascular events: reanalysis of the Women’s Health Initiative limited access dataset and meta-analysis. BMJ. 2011; 342: d2040.CrossRefPubMedPubMedCentral Bolland MJ, Grey A, Avenell A, Gamble GD, Reid IR. Calcium supplements with or without vitamin D and risk of cardiovascular events: reanalysis of the Women’s Health Initiative limited access dataset and meta-analysis. BMJ. 2011; 342: d2040.CrossRefPubMedPubMedCentral
5.
Zurück zum Zitat Cao Y, Winzenberg T, Nguo K, Lin J, Jones G, Ding C. Association between serum levels of 25-hydroxyvitamin D and osteoarthritis: a systematic review. Rheumatology (Oxford). 2013; 52(7): 1323-1334.CrossRef Cao Y, Winzenberg T, Nguo K, Lin J, Jones G, Ding C. Association between serum levels of 25-hydroxyvitamin D and osteoarthritis: a systematic review. Rheumatology (Oxford). 2013; 52(7): 1323-1334.CrossRef
6.
Zurück zum Zitat Cao Y, Winzenberg T, Nguo K, Lin J, Jones G, Ding C. Association between serum levels of 25-hydroxyvitamin D and osteoarthritis: a systematic review. Rheumatology. 2013; 52(7): 1323-1334.CrossRefPubMed Cao Y, Winzenberg T, Nguo K, Lin J, Jones G, Ding C. Association between serum levels of 25-hydroxyvitamin D and osteoarthritis: a systematic review. Rheumatology. 2013; 52(7): 1323-1334.CrossRefPubMed
7.
Zurück zum Zitat Felson DT. CORR Insights®: Does Vitamin D Improve Osteoarthritis of the Knee: A Randomized Controlled Pilot Trial. Clin Orthop Relat Res. 2013; 471(11): 3563-3564.CrossRefPubMedPubMedCentral Felson DT. CORR Insights®: Does Vitamin D Improve Osteoarthritis of the Knee: A Randomized Controlled Pilot Trial. Clin Orthop Relat Res. 2013; 471(11): 3563-3564.CrossRefPubMedPubMedCentral
8.
Zurück zum Zitat Felson DT, Niu J, Clancy M, et al. Low levels of vitamin D and worsening of knee osteoarthritis: results of two longitudinal studies. Arthritis Rheum. 2007; 56(1): 129-136.CrossRefPubMed Felson DT, Niu J, Clancy M, et al. Low levels of vitamin D and worsening of knee osteoarthritis: results of two longitudinal studies. Arthritis Rheum. 2007; 56(1): 129-136.CrossRefPubMed
9.
Zurück zum Zitat Gerwin N, Bendele AM, Glasson S, Carlson CS. The OARSI histopathology initiative—recommendations for histological assessments of osteoarthritis in the rat. Osteoarthritis Cartilage. 2010; 18(Suppl 3): S24-34.CrossRefPubMed Gerwin N, Bendele AM, Glasson S, Carlson CS. The OARSI histopathology initiative—recommendations for histological assessments of osteoarthritis in the rat. Osteoarthritis Cartilage. 2010; 18(Suppl 3): S24-34.CrossRefPubMed
10.
Zurück zum Zitat Goula T, Kouskoukis A, Drosos G, et al. Vitamin D status in patients with knee or hip osteoarthritis in a Mediterranean country. J Orthop Traumatol. 2015; 16(1): 35-39.CrossRefPubMed Goula T, Kouskoukis A, Drosos G, et al. Vitamin D status in patients with knee or hip osteoarthritis in a Mediterranean country. J Orthop Traumatol. 2015; 16(1): 35-39.CrossRefPubMed
11.
Zurück zum Zitat Helmick CG, Felson DT, Lawrence RC, et al. Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part I. Arthritis Rheum. 2008; 58(1): 15-25.CrossRefPubMed Helmick CG, Felson DT, Lawrence RC, et al. Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part I. Arthritis Rheum. 2008; 58(1): 15-25.CrossRefPubMed
12.
Zurück zum Zitat Lacourt M, Gao C, Li A, et al. Relationship between cartilage and subchondral bone lesions in repetitive impact trauma-induced equine osteoarthritis. Osteoarthritis Cartilage. 2012; 20(6): 572-583.CrossRefPubMed Lacourt M, Gao C, Li A, et al. Relationship between cartilage and subchondral bone lesions in repetitive impact trauma-induced equine osteoarthritis. Osteoarthritis Cartilage. 2012; 20(6): 572-583.CrossRefPubMed
13.
Zurück zum Zitat Lane NE, Gore LR, Cummings SR, et al. Serum vitamin D levels and incident changes of radiographic hip osteoarthritis: a longitudinal study. Study of Osteoporotic Fractures Research Group. Arthritis Rheum. 1999; 42(5): 854-860.CrossRefPubMed Lane NE, Gore LR, Cummings SR, et al. Serum vitamin D levels and incident changes of radiographic hip osteoarthritis: a longitudinal study. Study of Osteoporotic Fractures Research Group. Arthritis Rheum. 1999; 42(5): 854-860.CrossRefPubMed
14.
Zurück zum Zitat Lawrence RC, Felson DT, Helmick CG, et al. Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part II. Arthritis Rheum. 2008; 58(1): 26-35.CrossRefPubMedPubMedCentral Lawrence RC, Felson DT, Helmick CG, et al. Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part II. Arthritis Rheum. 2008; 58(1): 26-35.CrossRefPubMedPubMedCentral
15.
Zurück zum Zitat Lorenzo P, Bayliss MT, Heinegard D. Altered patterns and synthesis of extracellular matrix macromolecules in early osteoarthritis. Matrix Biol. 2004; 23(6): 381-391.CrossRefPubMed Lorenzo P, Bayliss MT, Heinegard D. Altered patterns and synthesis of extracellular matrix macromolecules in early osteoarthritis. Matrix Biol. 2004; 23(6): 381-391.CrossRefPubMed
16.
Zurück zum Zitat Malas FU, Kara M, Aktekin L, Ersoz M, Ozcakar L. Does vitamin D affect femoral cartilage thickness? An ultrasonographic study. Clin Rheumatol. 2014; 33(9): 1331-1334.CrossRefPubMed Malas FU, Kara M, Aktekin L, Ersoz M, Ozcakar L. Does vitamin D affect femoral cartilage thickness? An ultrasonographic study. Clin Rheumatol. 2014; 33(9): 1331-1334.CrossRefPubMed
17.
Zurück zum Zitat McAlindon TE, Felson DT, Zhang Y, et al. Relation of dietary intake and serum levels of vitamin D to progression of osteoarthritis of the knee among participants in the Framingham Study. Ann Intern Med. 1996; 125(5): 353-359.CrossRefPubMed McAlindon TE, Felson DT, Zhang Y, et al. Relation of dietary intake and serum levels of vitamin D to progression of osteoarthritis of the knee among participants in the Framingham Study. Ann Intern Med. 1996; 125(5): 353-359.CrossRefPubMed
18.
Zurück zum Zitat Mohan G, Perilli E, Kuliwaba JS, Humphries JM, Parkinson IH, Fazzalari NL. Application of in vivo micro-computed tomography in the temporal characterisation of subchondral bone architecture in a rat model of low-dose monosodium iodoacetate-induced osteoarthritis. Arthritis Res Ther. 2011; 13(6): R210.CrossRefPubMedPubMedCentral Mohan G, Perilli E, Kuliwaba JS, Humphries JM, Parkinson IH, Fazzalari NL. Application of in vivo micro-computed tomography in the temporal characterisation of subchondral bone architecture in a rat model of low-dose monosodium iodoacetate-induced osteoarthritis. Arthritis Res Ther. 2011; 13(6): R210.CrossRefPubMedPubMedCentral
19.
Zurück zum Zitat Pan J, Wang B, Li W, et al. Elevated cross-talk between subchondral bone and cartilage in osteoarthritic joints. Bone. 2012; 51(2): 212-217.CrossRefPubMed Pan J, Wang B, Li W, et al. Elevated cross-talk between subchondral bone and cartilage in osteoarthritic joints. Bone. 2012; 51(2): 212-217.CrossRefPubMed
20.
Zurück zum Zitat Pascual Garrido C, Hakimiyan AA, Rappoport L, Oegema TR, Wimmer MA, Chubinskaya S. Anti-apoptotic treatments prevent cartilage degradation after acute trauma to human ankle cartilage. Osteoarthritis Cartilage. 2009; 17(9): 1244-1251.CrossRefPubMed Pascual Garrido C, Hakimiyan AA, Rappoport L, Oegema TR, Wimmer MA, Chubinskaya S. Anti-apoptotic treatments prevent cartilage degradation after acute trauma to human ankle cartilage. Osteoarthritis Cartilage. 2009; 17(9): 1244-1251.CrossRefPubMed
21.
Zurück zum Zitat Pearson RG, Kurien T, Shu KS, Scammell BE. Histopathology grading systems for characterisation of human knee osteoarthritis—reproducibility, variability, reliability, correlation, and validity. Osteoarthritis Cartilage. 2011; 19(3): 324-331.CrossRefPubMed Pearson RG, Kurien T, Shu KS, Scammell BE. Histopathology grading systems for characterisation of human knee osteoarthritis—reproducibility, variability, reliability, correlation, and validity. Osteoarthritis Cartilage. 2011; 19(3): 324-331.CrossRefPubMed
22.
Zurück zum Zitat Sacks JJ, Luo Y-H, Helmick CG. Prevalence of specific types of arthritis and other rheumatic conditions in the ambulatory health care system in the United States, 2001–2005. Arthritis Care Res. 2010; 62(4): 460-464.CrossRef Sacks JJ, Luo Y-H, Helmick CG. Prevalence of specific types of arthritis and other rheumatic conditions in the ambulatory health care system in the United States, 2001–2005. Arthritis Care Res. 2010; 62(4): 460-464.CrossRef
23.
Zurück zum Zitat Sanghi D, Mishra A, Sharma AC, et al. Does vitamin D improve osteoarthritis of the knee: a randomized controlled pilot trial. Clin Orthop Relat Res. 2013; 471(11): 3556-3562.CrossRefPubMedPubMedCentral Sanghi D, Mishra A, Sharma AC, et al. Does vitamin D improve osteoarthritis of the knee: a randomized controlled pilot trial. Clin Orthop Relat Res. 2013; 471(11): 3556-3562.CrossRefPubMedPubMedCentral
24.
Zurück zum Zitat Squires GR, Okouneff S, Ionescu M, Poole AR. The pathobiology of focal lesion development in aging human articular cartilage and molecular matrix changes characteristic of osteoarthritis. Arthritis Rheum. 2003; 48(5): 1261-1270.CrossRefPubMed Squires GR, Okouneff S, Ionescu M, Poole AR. The pathobiology of focal lesion development in aging human articular cartilage and molecular matrix changes characteristic of osteoarthritis. Arthritis Rheum. 2003; 48(5): 1261-1270.CrossRefPubMed
25.
Zurück zum Zitat Stubendorff JJ, Lammentausta E, Struglics A, Lindberg L, Heinegard D, Dahlberg LE. Is cartilage sGAG content related to early changes in cartilage disease? Implications for interpretation of dGEMRIC. Osteoarthritis Cartilage. 2012; 20(5): 396-404.CrossRefPubMed Stubendorff JJ, Lammentausta E, Struglics A, Lindberg L, Heinegard D, Dahlberg LE. Is cartilage sGAG content related to early changes in cartilage disease? Implications for interpretation of dGEMRIC. Osteoarthritis Cartilage. 2012; 20(5): 396-404.CrossRefPubMed
26.
Zurück zum Zitat Tare M, Emmett SJ, Coleman HA, et al. Vitamin D insufficiency is associated with impaired vascular endothelial and smooth muscle function and hypertension in young rats. J Physiol. 2011; 589(Pt 19): 4777-4786.CrossRefPubMedPubMedCentral Tare M, Emmett SJ, Coleman HA, et al. Vitamin D insufficiency is associated with impaired vascular endothelial and smooth muscle function and hypertension in young rats. J Physiol. 2011; 589(Pt 19): 4777-4786.CrossRefPubMedPubMedCentral
27.
Zurück zum Zitat Tetlow LC, Woolley DE. Expression of vitamin D receptors and matrix metalloproteinases in osteoarthritic cartilage and human articular chondrocytes in vitro. Osteoarthritis Cartilage. 2001; 9(5): 423-431.CrossRefPubMed Tetlow LC, Woolley DE. Expression of vitamin D receptors and matrix metalloproteinases in osteoarthritic cartilage and human articular chondrocytes in vitro. Osteoarthritis Cartilage. 2001; 9(5): 423-431.CrossRefPubMed
28.
Zurück zum Zitat Troeberg L, Nagase H. Proteases involved in cartilage matrix degradation in osteoarthritis. Biochim Biophys Acta. 2012; 1824(1): 133-145.CrossRefPubMed Troeberg L, Nagase H. Proteases involved in cartilage matrix degradation in osteoarthritis. Biochim Biophys Acta. 2012; 1824(1): 133-145.CrossRefPubMed
29.
Zurück zum Zitat van der Sluijs JA, Geesink RG, van der Linden AJ, Bulstra SK, Kuyer R, Drukker J. The reliability of the Mankin score for osteoarthritis. J Orthop Res. 1992; 10(1): 58-61.CrossRefPubMed van der Sluijs JA, Geesink RG, van der Linden AJ, Bulstra SK, Kuyer R, Drukker J. The reliability of the Mankin score for osteoarthritis. J Orthop Res. 1992; 10(1): 58-61.CrossRefPubMed
Metadaten
Titel
Low Levels of Vitamin D have a Deleterious Effect on the Articular Cartilage in a Rat Model
verfasst von
Cecilia Pascual-Garrido, MD
Michael E. Angeline, MD
Richard Ma, MD
Jorge Chahla, MD
Cliff Voigt, MD
Xiang Hua Deng, MD
Joseph Nguyen, MPH
Russell F. Warren, MD
Scott A. Rodeo, MD
Publikationsdatum
10.03.2016
Verlag
Springer US
Erschienen in
HSS Journal ® / Ausgabe 2/2016
Print ISSN: 1556-3316
Elektronische ISSN: 1556-3324
DOI
https://doi.org/10.1007/s11420-016-9492-x

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