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Erschienen in: Journal of Gastroenterology 1/2012

01.01.2012 | Original Article—Alimentary Tract

Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer

verfasst von: Ji-Han Jung, Hye-Jung Kim, Jeonghun Yeom, Changyoung Yoo, Jihye Shin, Jinyoung Yoo, Chang Suk Kang, Cheolju Lee

Erschienen in: Journal of Gastroenterology | Ausgabe 1/2012

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Abstract

Background

Lymph node metastasis (LNM) is recognized as an important factor in the progression of tumor malignancy. It is required to discover molecular markers for the prediction of LNM in gastric cancers (GCs).

Methods

An isotope coded affinity tag (ICAT) method and mass spectrometry were used for the quantitative profiling of LNM-related proteins. Western blot analysis of the identified proteins and immunohistochemistry on a tissue microarray comprising 120 GC cases were performed for validation.

Results

We identified 151 differentially expressed proteins (DEPs) with an abundance ratio greater than 1.5-fold. The proteins upregulated in LNM-negative GCs were largely populated with proteins related to cell death. Among the DEPs, galectin-2 was further tested because its expression level was significantly higher in LNM-negative GCs (~12-fold, p < 0.0001) and its expression is known to be not ubiquitous but confined to the gastrointestinal tract. Immunohistochemical analysis revealed that low expression of galectin-2 was significantly associated with LNM (p = 0.031) and advanced clinical stage (p = 0.024). The association of low galectin-2 with LNM was found even in early GCs (p = 0.020).

Conclusion

Our results show that proteomic analysis coupled with immunohistochemistry using tissue microarray is a useful tool for identifying LNM-associated proteins in GC. Also, loss of galectin-2 might play an important role in the aggressiveness of GC.
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Metadaten
Titel
Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer
verfasst von
Ji-Han Jung
Hye-Jung Kim
Jeonghun Yeom
Changyoung Yoo
Jihye Shin
Jinyoung Yoo
Chang Suk Kang
Cheolju Lee
Publikationsdatum
01.01.2012
Verlag
Springer Japan
Erschienen in
Journal of Gastroenterology / Ausgabe 1/2012
Print ISSN: 0944-1174
Elektronische ISSN: 1435-5922
DOI
https://doi.org/10.1007/s00535-011-0463-1

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