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European Spine Journal

Erschienen in:

14.07.2018 | Original Article

Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells

verfasst von: Ming-Bo Guo, De-Chun Wang, Hai-Fei Liu, Long-Wei Chen, Jian-Wei Wei, Yong Lin, Hui Xue

Erschienen in: European Spine Journal | Ausgabe 10/2018

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Abstract

Purpose

This study aimed to investigate the potential mechanism and value of lupeol in inhibiting high-glucose-induced apoptosis in rabbit nucleus pulposus cells (NPCs).

Methods

NPCs were divided into four groups: control (CON), high glucose (HG), LUP, and HG + LUP. Viability, reactive oxygen species (ROS) levels, and apoptosis were examined in NPCs. The protein expression levels of Bax, Bcl-2, cytochrome C, and caspase 9/3 were measured using reverse transcription–polymerase chain reaction and Western blot assay.

Results

The apoptotic rate and total ROS level of the HG group significantly increased compared with the CON group (P < 0.01). The total ROS level in the HG + LUP group significantly decreased compared with the HG group(P < 0.05). The mRNA expression of Bcl-2 was significantly upregulated, whereas the expression of Bax, cytochrome C, and caspase 9/3 was downregulated in the HG + LUP group compared with those in the HG group(P < 0.05).The Western blot assay showed that the expression of Bcl-2 was upregulated, but the expression of Bax, cytochrome C, and caspase 9/3 was significantly downregulated in the HG + LUP group compared with the HG group (P < 0.05).

Conclusions

Lupeol inhibited high-glucose-induced apoptosis in NPCs by enhancing the anti-oxidative stress in the mitochondria. This study suggested lupeol as a potential therapeutic drug for treating intervertebral disc degeneration under hyperglycaemic conditions.

Graphical abstract

These slides can be retrieved under Electronic Supplementary Material.

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Titel: Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells
verfasst von: Ming-Bo Guo
De-Chun Wang
Hai-Fei Liu
Long-Wei Chen
Jian-Wei Wei
Yong Lin
Hui Xue
Publikationsdatum: 14.07.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: European Spine Journal / Ausgabe 10/2018
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI: https://doi.org/10.1007/s00586-018-5687-9

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Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells

Abstract

Purpose

Methods

Results

Conclusions

Graphical abstract

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Springer Medizin

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Purpose

Methods

Results

Conclusions

Graphical abstract

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