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European Archives of Oto-Rhino-Laryngology

Erschienen in:

01.06.2014 | Laryngology

Luteolin induces apoptosis by activating Fas signaling pathway at the receptor level in laryngeal squamous cell line Hep-2 cells

verfasst von: Hui Zhang, Xiuguo Li, Yuanyuan Zhang, Xinyong Luan

Erschienen in: European Archives of Oto-Rhino-Laryngology | Ausgabe 6/2014

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Abstract

Luteolin, a naturally occurring flavonoid, possesses anti-cancer activities against several human cancers, but the exact molecular and biochemical mechanisms of above findings are not very clear, and its activity against head and neck squamous cell carcinoma (HNSCC) is seldom mentioned. In this study, we investigated luteolin against human laryngeal squamous cell line Hep-2 cells, using MTT assay, flow cytometry, Western blot and reverse transcriptase–polymerase chain reaction (RT-PCR) analysis. Luteolin inhibited Hep-2 cells proliferation at the inhibitive concentrations of 50 % (IC₅₀) near to 50 μM and induced the apoptosis in Hep-2 cells through caspase-3 and caspase-8 activation. Up-regulation of Fas and down-regulation of long form cellular FLICE-like inhibitory protein (c-FLIP_L) protein were also involved after luteolin treatment at both protein and mRNA levels. Luteolin could not only inhibit cell proliferation but also induce apoptosis by activating the Fas signaling pathway at the receptor level in laryngeal squamous cell line Hep-2 cells.

Yang RY, Lin S, Kuo G (2008) Content and distribution of flavonoids among 91 edible plant species. Asia Pac J Clin Nutr. 17(Suppl 1):275–279PubMed

Imai M, Kikuchi H, Denda T et al (2009) Cytotoxic effects of flavonoids against a human colon cancer derived cell line, COLO 201: a potential natural anti-cancer substance. Cancer Lett 276:74–80PubMedCrossRef

Terao J, Kawai Y, Murota K (2008) Vegetable flavonoids and cardiovascular disease. Asia Pac J Clin Nutr 17(Suppl 1):291–293PubMed

Simons R, Gruppen H, Bovee TF et al (2012) Prenylated isoflavonoids from plants as selective estrogen receptor modulators (phytoSERMs). Food Funct 3(8):810–827PubMedCrossRef

Meeran SM, Vaid M, Punathil T, Katiyar SK (2009) Dietary grape seed proanthocyanidins inhibit 12-O-tetradecanoyl phorbol-13-acetate-caused skin tumor promotion in 7,12-dimethylbenz[a]anthracene-initiated mouse skin, which is associated with the inhibition of inflammatory responses. Carcinogenesis 30(3):520–528PubMedCrossRef

Hu X, Moscinski LC (2011) Cdc2: a monopotent or pluripotent CDK? Cell Prolif 44(3):205–211PubMedCrossRef

Chow AK, Ng L, Sing Li H et al (2012) Anti-tumor efficacy of a recombinant human arginase in human hepatocellular carcinoma. Curr Cancer Drug Targets 12(9):1233–1243PubMed

Lange F, Brandt B, Tiedge M et al (2009) Galectin-1 induced activation of the mitochondrial apoptotic pathway: evidence for a connection between death-receptor and mitochondrial pathways in human Jurkat T lymphocytes. Histochem Cell Biol 132(2):211–223PubMedCrossRef

Zhang M, Lee SJ, An C et al (2011) Caveolin-1 mediates Fas-BID signaling in hyperoxia-induced apoptosis. Free Radical Biol Med 50(10):1252–1262CrossRef

10.

Xu B, Xu Z, Xia T et al (2011) Effects of the Fas/Fas-L pathway on fluoride-induced apoptosis in SH-SY5Y cells. Environ Toxicol 26(1):86–92PubMedCrossRef

11.

Konstantakou EG, Voutsinas GE, Karkoulis PK et al (2009) Human bladder cancer cells undergo cisplatin-induced apoptosis that is associated with p53-dependent and p53-independent responses. Int J Oncol 35(2):401–416PubMed

12.

Sproll KC, Ballo H, Hoffmann TK et al (2009) Is there a role for the Fas-/Fas-Ligand pathway in chemoresistance of human squamous cell carcinomas of the head and neck (SCCHN)? Oral Oncol 45(1):69–84PubMedCrossRef

13.

Subramaniam K, Hirpara JL, Tucker-Kellogg L et al (2013) FLIP: a flop for execution signals. Cancer Lett 332(2):151–155PubMedCrossRef

14.

Hotta T, Suzuki H, Nagai S et al (2003) Chemotherapeutic agents sensitize sarcoma cell lines to tumor necrosis factor-related apoptosis-inducing ligand-induced caspase-8 activation, apoptosis and loss of mitochondrial membrane potential. J Orthop Res 21(5):949–957PubMedCrossRef

15.

Grobner S, Adkins I, Schulz S et al (2007) Catalytically active Yersinia outer protein P induces cleavage of RIP and caspase-8 at the level of the DISC independently of death receptors in dendritic cells. Apoptosis 12(10):1813–1825PubMedCrossRef

16.

Rasper DM, Vaillancourt JP, Hadano S et al (1998) Cell death attenuation by ‘Usurpin’, a mammalian DED-caspase homologue that precludes caspase-8 recruitment and activation by the CD-95 (Fas, APO-1) receptor complex. Cell Death Differ 5(4):271–288PubMedCrossRef

17.

Lim JH, Park JW, Choi KS et al (2009) Rottlerin induces apoptosis via death receptor 5 (DR5) up-regulation through CHOP-dependent and PKC delta-independent mechanism in human malignant tumor cells. Carcinogenesis 30(5):729–736PubMedCrossRef

18.

Subramaniam A, Shanmugam MK, Perumal E et al (2013) Potential role of signal transducer and activator of transcription (STAT)3 signaling pathway in inflammation, survival, proliferation and invasion of hepatocellular carcinoma. Biochimica biophysica acta 1835:46–60

19.

Wang L, Azad N, Kongkaneramit L et al (2008) The Fas death signaling pathway connecting reactive oxygen species generation and FLICE inhibitory protein down-regulation. J Immunol 180(5):3072–3080PubMedCentralPubMedCrossRef

20.

Bhattacharya S, Haldar PK (2012) Chemopreventive property of Trichosanthes dioica root against 3-methylcholanthrene-induced carcinogenesis in albino mice. J Environ Pathol Toxicol Oncol 31(2):109–119PubMedCrossRef

21.

Kaulfuss S, Burfeind P, Gaedcke J, Scharf JG (2009) Dual silencing of insulin-like growth factor-I receptor and epidermal growth factor receptor in colorectal cancer cells is associated with decreased proliferation and enhanced apoptosis. Mol Cancer Ther 8(4):821–833PubMedCrossRef

22.

Kim BM, Choi YJ, Han Y et al (2009) N, N-dimethyl phytosphingosine induces caspase-8-dependent cytochrome c release and apoptosis through ROS generation in human leukemia cells. Toxicol Appl Pharmacol 239(1):87–97PubMedCrossRef

23.

Kim MJ, Choi SY, Park IC et al (2008) Opposing roles of c-Jun NH2-terminal kinase and p38 mitogen-activated protein kinase in the cellular response to ionizing radiation in human cervical cancer cells. Mol Cancer Res 6(11):1718–1731PubMedCrossRef

24.

Markaverich BM, Vijjeswarapu M, Shoulars K, Rodriguez M (2010) Luteolin and gefitinib regulation of EGF signaling pathway and cell cycle pathway genes in PC-3 human prostate cancer cells. J Steroid Biochem Mol Biol 122(4):219–231PubMedCentralPubMedCrossRef

25.

Roy A, Das BB, Ganguly A et al (2008) An insight into the mechanism of inhibition of unusual bi-subunit topoisomerase I from Leishmania donovani by 3,3′-di-indolylmethane, a novel DNA topoisomerase I poison with a strong binding affinity to the enzyme. Biochem J 409(2):611–622PubMedCrossRef

26.

Brumatti G, Yon M, Castro FA et al (2008) Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res 314(3):554–563PubMedCrossRef

27.

Pforr C, Neumann L, Eils R et al (2011) Understanding life and death at CD95. Adv Exp Med Biol 691:151–161PubMedCrossRef

28.

Iwase M, Takaoka S, Uchida M et al (2008) Epidermal growth factor receptor inhibitors enhance susceptibility to Fas-mediated apoptosis in oral squamous cell carcinoma cells. Oral Oncol 44(4):361–368PubMedCrossRef

29.

Safa AR, Day TW, Wu CH (2008) Cellular FLICE-like inhibitory protein (C-FLIP): a novel target for cancer therapy. Curr Cancer Drug Targets 8(1):37–46PubMedCrossRef

30.

Diessenbacher P, Hupe M, Sprick MR et al (2008) NF-kappa B inhibition reveals differential mechanisms of TNF versus TRAIL-induced apoptosis upstream or at the level of caspase-8 activation independent of cIAP2. J Invest Dermatol 128(5):1134–1147PubMedCrossRef

31.

Fulda S (2013) Targeting c-FLICE-like inhibitory protein (CFLAR) in cancer. Expert Opin Ther Targets 17(2):195–201PubMedCrossRef

32.

Kim MO, Kim MH, Lee SH et al (2009) 5′-N-ethylcarboxamide induces IL-6 expression via MAPKs and NF-kappaB activation through Akt, Ca(2 +)/PKC, cAMP signaling pathways in mouse embryonic stem cells. J Cell Physiol 219(3):752–759PubMedCrossRef

33.

Day TW, Najafi F, Wu CH, Safa AR (2006) Cellular FLICE-like inhibitory protein (c-FLIP): a novel target for Taxol-induced apoptosis. Biochem Pharmacol 71(11):1551–1561PubMedCrossRef

34.

Llobet D, Eritja N, Encinas M et al (2008) CK2 controls TRAIL and Fas sensitivity by regulating FLIP levels in endometrial carcinoma cells. Oncogene 27(18):2513–2524PubMedCrossRef

35.

Kreuz S, Siegmund D, Scheurich P, Wajant H (2001) NF-kappaB inducers upregulate cFLIP, a cycloheximide-sensitive inhibitor of death receptor signaling. Mol Cell Biol 21(12):3964–3973PubMedCentralPubMedCrossRef

36.

Panka DJ, Mano T, Suhara T et al (2001) Phosphatidylinositol 3-kinase/Akt activity regulates c-FLIP expression in tumor cells. J Biol Chem 276(10):6893–6896PubMedCrossRef

37.

Ruiz PA, Haller D (2006) Functional diversity of flavonoids in the inhibition of the proinflammatory NF-kappaB, IRF, and Akt signaling pathways in murine intestinal epithelial cells. J Nutr 136(3):664–671PubMed

38.

Horinaka M, Yoshida T, Shiraishi T et al (2005) Luteolin induces apoptosis via death receptor 5 upregulation in human malignant tumor cells. Oncogene 24(48):7180–7189PubMedCrossRef

39.

Fu J, Chen D, Zhao B et al (2012) Luteolin induces carcinoma cell apoptosis through binding Hsp90 to suppress constitutive activation of STAT3. PLoS One 7(11):e49194PubMedCentralPubMedCrossRef

40.

Haag C, Stadel D, Zhou S et al (2011) Identification of c-FLIP(L) and c-FLIP(S) as critical regulators of death receptor-induced apoptosis in pancreatic cancer cells. Gut 60(2):225–237PubMedCrossRef

41.

Kinoshita H, Yoshikawa H, Shiiki K et al (2000) Cisplatin (CDDP) sensitizes human osteosarcoma cell to Fas/CD95-mediated apoptosis by down-regulating FLIP-L expression. Int J Cancer 88(6):986–991PubMedCrossRef

42.

Mendenhall WM, Parsons JT, Stringer SP et al (1988) T1-T2 vocal cord carcinoma: a basis for comparing the results of radiotherapy and surgery[J]. Head Neck Surg 10(6):373–377PubMedCrossRef

43.

Persky MS, Lagmay VM, Cooper J et al (2000) Curative radiotherapy for anterior commissure laryngeal carcinoma. Ann Otol Rhinol Laryngol 109(2):156–159PubMed

Titel: Luteolin induces apoptosis by activating Fas signaling pathway at the receptor level in laryngeal squamous cell line Hep-2 cells
verfasst von: Hui Zhang
Xiuguo Li
Yuanyuan Zhang
Xinyong Luan
Publikationsdatum: 01.06.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: European Archives of Oto-Rhino-Laryngology / Ausgabe 6/2014
Print ISSN: 0937-4477
Elektronische ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-014-2903-z

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Luteolin induces apoptosis by activating Fas signaling pathway at the receptor level in laryngeal squamous cell line Hep-2 cells

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