Skip to main content
main-content

01.12.2012 | Research | Ausgabe 1/2012 Open Access

Journal of Inflammation 1/2012

Lymphocyte Depletion in Experimental Hemorrhagic Shock in Swine

Zeitschrift:
Journal of Inflammation > Ausgabe 1/2012
Autoren:
Jason S Hawksworth, Christopher Graybill, Trevor S Brown, Suzanne M Gillern, Shannon M Wallace, Thomas A Davis, Eric A Elster, Doug K Tadaki
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1476-9255-9-34) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

Conceived and designed the experiments: EE DT. Performed the experiments: JSH JCG SG TB. Analyzed the data: JSH JCG TB SW TD EE JSH SG. Wrote the paper: JSH JCG. Supervised the protocol that obtained the information that lead to the writing of the manuscript: TB TD DT EE. Final revisions of the manuscript: DT TB TD EE. All authors read and approved the final manuscript

Abstract

Background

Hemorrhagic shock results in systemic activation of the immune system and leads to ischemia-reperfusion injury. Lymphocytes have been identified as critical mediators of the early innate immune response to ischemia-reperfusion injury, and immunomodulation of lymphocytes may prevent secondary immunologic injury in surgical and trauma patients.

Methods

Yorkshire swine were anesthetized and underwent a grade III liver injury with uncontrolled hemorrhage to induce hemorrhagic shock. Experimental groups were treated with a lymphocyte depletional agent, porcine polyclonal anti-thymocyte globulin (PATG) (n = 8) and compared to a vehicle control group (n = 9). Animals were observed over a 3 day survival period. Circulating lymphocytes were examined with FACS analysis for CD3/CD4/CD8, and central lymphocytes with mesenteric lymph node and spleen staining for CD3. Circulating and lung tissue16 infiltrating neutrophils were measured. Circulating CD3 lymphocytes in the blood and in central lymphoid organs (spleen/lymph node) were stained and evaluated using FACS analysis. Immune-related gene expression from liver tissue was quantified using RT-PCR.

Results

The overall survival was 22% (2/9) in the control and 75% (6/8) in the PATG groups, p = 0.09; during the reperfusion period (following hemorrhage) survival was 25% (2/8) in the control and 100% (6/6) in the PATG groups, p = 0.008. Mean blood loss and hemodynamic profiles were not significantly different between the experimental and control groups. Circulating CD3+CD4+ lymphocytes were significantly depleted in the PATG group compared to control. Lymphocyte depletion in the setting of hemorrhagic shock also significantly decreased circulating and lung tissue infiltrating neutrophils, and decreased expression of liver ischemia gene expression.

Conclusions

Lymphocyte manipulation with a depletional (PATG) strategy improves reperfusion survival in experimental hemorrhagic shock using a porcine liver injury model. This proof of principle study paves the way for further development of immunomodulation approaches to ameliorate secondary immune injury following hemorrhagic shock.
Zusatzmaterial
Authors’ original file for figure 1
12950_2012_263_MOESM1_ESM.pdf
Authors’ original file for figure 2
12950_2012_263_MOESM2_ESM.tiff
Authors’ original file for figure 3
12950_2012_263_MOESM3_ESM.pdf
Authors’ original file for figure 4
12950_2012_263_MOESM4_ESM.pdf
Authors’ original file for figure 5
12950_2012_263_MOESM5_ESM.pdf
Authors’ original file for figure 6
12950_2012_263_MOESM6_ESM.tiff
Authors’ original file for figure 7
12950_2012_263_MOESM7_ESM.pdf
Authors’ original file for figure 8
12950_2012_263_MOESM8_ESM.tiff
Authors’ original file for figure 9
12950_2012_263_MOESM9_ESM.pdf
Authors’ original file for figure 10
12950_2012_263_MOESM10_ESM.tiff
Authors’ original file for figure 11
12950_2012_263_MOESM11_ESM.tiff
Authors’ original file for figure 12
12950_2012_263_MOESM12_ESM.tiff
Authors’ original file for figure 13
12950_2012_263_MOESM13_ESM.tiff
Authors’ original file for figure 14
12950_2012_263_MOESM14_ESM.tiff
Authors’ original file for figure 15
12950_2012_263_MOESM15_ESM.tiff
Authors’ original file for figure 16
12950_2012_263_MOESM16_ESM.tiff
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2012

Journal of Inflammation 1/2012 Zur Ausgabe

Neu im Fachgebiet Innere Medizin


 

Meistgelesene Bücher aus der Inneren Medizin

2017 | Buch

Rheumatologie aus der Praxis

Entzündliche Gelenkerkrankungen – mit Fallbeispielen

Dieses Fachbuch macht mit den wichtigsten chronisch entzündlichen Gelenk- und Wirbelsäulenerkrankungen vertraut. Anhand von über 40 instruktiven Fallbeispielen werden anschaulich diagnostisches Vorgehen, therapeutisches Ansprechen und der Verlauf …

Herausgeber:
Rudolf Puchner

2016 | Buch

Ambulant erworbene Pneumonie

Was, wann, warum – Dieses Buch bietet differenzierte Diagnostik und Therapie der ambulant erworbenen Pneumonie zur sofortigen sicheren Anwendung. Entsprechend der neuesten Studien und Leitlinien aller wichtigen Fachgesellschaften.

Herausgeber:
Santiago Ewig

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Innere Medizin und bleiben Sie gut informiert – ganz bequem per eMail.

© Springer Medizin 

Bildnachweise