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Erschienen in: BMC Infectious Diseases 3/2014

Open Access 01.05.2014 | ePoster presentation

M. tuberculosis Peptide based Point-of care test for TB

verfasst von: Jesus M Gonzalez, Bryan Francis, Sherri T Burda, R Sam Niedbala, Suman Laal

Erschienen in: BMC Infectious Diseases | Sonderheft 3/2014

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Background

A rapid, simple and affordable point-of care (POC) test that can be implemented in peripheral health care settings to replace/improve upon microscopy for TB diagnosis or to rule out TB (triage) remains unmet.

Methods

Immunodominant regions of 3 Mycobacterium tuberculosis specific cell–wall proteins that are highly immunogenic in HIV- TB+ and HIV+TB+ patients have been mapped. Antibodies to these regions are absent in subjects with latent TB, BCG vaccination, other pulmonary diseases. To examine the feasibility of devising a peptide based rapid POC test, selected peptides were conjugated to a carrier protein using standard EDC/NHS coupling chemistry, purified by size exclusion; the peptide conjugation confirmed using MALDI MSMS before adsorption onto colloidal gold and striping onto nitrocellulose. The assay parameters were adjusted to determine positive/negative status within 15 minutes via visual or instrumented assessment.

Results

The prototype POC test was evaluated with sera from ~400 TB patients, non TB subjects from the groups defined in the methods section. The current prototype POC test provides >90% sensitivity and specificity in the above populations.

Conclusion

WHO recommends against use of all current commercial serological TB tests since they fail to achieve adequate sensitivity and specificity. These results demonstrate that, as for HIV rapid tests, carefully selected immunodominant epitopes of highly antigenic M. tuberculosis specific proteins can be the basis of antibody detection based rapid POC TB test. Test optimization to improve accuracy to meet WHO specifications for a POC test to replace microscopy or a triage test to rule-out TB are ongoing.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
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Metadaten
Titel
M. tuberculosis Peptide based Point-of care test for TB
verfasst von
Jesus M Gonzalez
Bryan Francis
Sherri T Burda
R Sam Niedbala
Suman Laal
Publikationsdatum
01.05.2014
Verlag
BioMed Central
Erschienen in
BMC Infectious Diseases / Ausgabe Sonderheft 3/2014
Elektronische ISSN: 1471-2334
DOI
https://doi.org/10.1186/1471-2334-14-S3-E27

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