nach oben

Clinical Oral Investigations

Erschienen in:

10.11.2018 | Original Article

Macrophage behavior and interplay with gingival fibroblasts cultured on six commercially available titanium, zirconium, and titanium-zirconium dental implants

verfasst von: Yulan Wang, Yufeng Zhang, Anton Sculean, Dieter D Bosshardt, Richard J Miron

Erschienen in: Clinical Oral Investigations | Ausgabe 8/2019

Einloggen, um Zugang zu erhalten

Abstract

Objectives

The host-material interface has been a crucial relationship dictating the successful integration of biomaterials, including dental implants. The aim of the present study was to first investigate how macrophages behaved on various dental implant surfaces and thereafter to investigate their effect on soft tissue cells.

Materials and methods

Macrophage adhesion, proliferation, and polarization towards either an M1 or M2 phenotype were investigated on six implant surfaces fabricated from pure titanium (Ti), pure zirconium (ZLA), and a titanium-zirconium (Ti-Zi) alloy of various surface topographies/chemistries. Thereafter, conditioned media (CM) collected from macrophages seeded on these various implant surfaces was cultured with murine gingival fibroblasts and investigated for their ability to promote collagen synthesis.

Results

Macrophages attached and proliferated in similar levels on all implant surfaces; however, the modSLA hydrophilic surfaces tended to decrease the pro-inflammatory response by lowering the gene expression of TNF-alpha, IL-1, and IL-6 and promoting tissue resolution through the expression of an M2-macrophage cytokine IL-10. Thereafter, CM from macrophages were seeded with gingival fibroblasts on each implant surface. In general, CM from macrophages significantly promoted gingival fibroblast cell attachment on all implant surfaces at either 4 or 8 h and, most notably, significantly promoted fibronectin and TGF-beta gene expression on both Ti and Ti-Zi hydrophilic surfaces.

Conclusions and clinical relevance

The present study found that implant surface topography and chemistry substantially impacted macrophage behavior. Most notably, modifications via hydrophilicity to both the pure Ti and Ti-Zi were shown to favor the secretion of macrophage pro-resolution markers and favored subsequent gingival fibroblast cell behavior when cultured with CM, whereas surface composition (Ti vs ZLA vs Ti-Zi) had little effect on macrophage polarization or gingival fibroblast behavior. This finding suggests that surface hydrophilicity would improve the soft tissue integration of dental implants, irrespective of material composition.

Miron RJ, Bosshardt DD (2016) OsteoMacs: key players around bone biomaterials. Biomaterials 82:1–19. https://doi.org/10.1016/j.biomaterials.2015.12.017 CrossRefPubMed

Romeo E, Lops D, Margutti E, Ghisolfi M, Chiapasco M, Vogel G (2003) Long-term survival and success of oral implants in the treatment of full and partial arches: a 7-year prospective study with the ITI dental implant system. Int J Oral Maxillofac Implants 19:247–259

Karoussis IK, Salvi GE, Heitz-Mayfield LJA, Brägger U, Hämmerle CHF, Lang NP (2003) Long-term implant prognosis in patients with and without a history of chronic periodontitis: a 10-year prospective cohort study of the ITI® dental implant system. Clin Oral Implants Res 14:329–339CrossRefPubMed

Eckert SE, Choi Y-G, Sánchez AR, Koka S (2006) Comparison of dental implant systems: quality of clinical evidence and prediction of 5-year survival. J Prosthet Dent 95:170CrossRef

Yi HY, Park YS, Pippenger BE, Lee B, Miron RJ, Dard M (2017) Dimensional changes following immediate and delayed implant placement: a histomorphometric study in the canine. Int J Oral Maxillofac Implants 32:541–546. https://doi.org/10.11607/jomi.5145 CrossRefPubMed

Altinci P, Can G, Gunes O, Ozturk C, Eren H (2016) Stability and marginal bone level changes of SLActive titanium-zirconium implants placed with flapless surgery: a prospective pilot study. Clin Implant Dent Relat Res 18:1193–1199. https://doi.org/10.1111/cid.12392 CrossRefPubMed

Cabrera-Dominguez J, Castellanos-Cosano L, Torres-Lagares D, Machuca-Portillo G (2017) A prospective case-control clinical study of titanium-zirconium alloy implants with a hydrophilic surface in patients with type 2 diabetes mellitus. Int J Oral Maxillofac Implants 32:1135–1144. https://doi.org/10.11607/jomi.5577 CrossRefPubMed

Herrmann J, Hentschel A, Glauche I, Vollmer A, Schlegel KA, Lutz R (2016) Implant survival and patient satisfaction of reduced diameter implants made from a titanium-zirconium alloy: a retrospective cohort study with 550 implants in 311 patients. J Craniomaxillofac Surg 44:1940–1944. https://doi.org/10.1016/j.jcms.2016.09.007 CrossRefPubMed

Schincaglia GP, Kim YK, Piva R, Sobue T, Torreggiani E, Kalajzic I (2017) Osteogenesis during early healing around titanium and Roxolid implants: evaluation of bone markers by immunohistochemistry and RT-PCR analysis in miniature pigs: a pilot study. Int J Oral Maxillofac Implants 32:42–51. https://doi.org/10.11607/jomi.4859 CrossRefPubMed

10.

Kniha K, Milz S, Kniha H, Ayoub N, Holzle F, Modabber A (2018) Peri-implant Crestal bone changes around zirconia implants in periodontally healthy and compromised patients. Int J Oral Maxillofac Implants 33:217–222. https://doi.org/10.11607/jomi.5598 CrossRefPubMed

11.

Kniha K, Modabber A, Kniha H, Mohlhenrich SC, Holzle F, Milz S (2018) Dimensions of hard and soft tissue around adjacent, compared with single-tooth, zirconia implants. Br J Oral Maxillofac Surg 56:43–47. https://doi.org/10.1016/j.bjoms.2017.11.005 CrossRefPubMed

12.

Trindade R, Albrektsson T, Tengvall P, Wennerberg A (2016) Foreign body reaction to biomaterials: on mechanisms for buildup and breakdown of osseointegration. Clin Implant Dent Relat Res 18:192–203. https://doi.org/10.1111/cid.12274 CrossRefPubMed

13.

Trindade R, Albrektsson T, Galli S, Prgomet Z, Tengvall P, Wennerberg A (2017) Osseointegration and foreign body reaction: titanium implants activate the immune system and suppress bone resorption during the first 4 weeks after implantation. Clin Implant Dent Relat Res 20:82–91. https://doi.org/10.1111/cid.12578 CrossRefPubMed

14.

Wang Y, Zhang Y, Miron RJ (2015) Health, maintenance, and recovery of soft tissues around implants. Clin Implant Dent Relat Res 18:618–634. https://doi.org/10.1111/cid.12343 CrossRefPubMed

15.

Sculean A, Gruber R, Bosshardt DD (2014) Soft tissue wound healing around teeth and dental implants. J Clin Periodontol 41(Suppl 15):S6–S22. https://doi.org/10.1111/jcpe.12206 CrossRefPubMed

16.

Lee H-J, Lee J, Lee J-T, Hong J-S, Lim B-S, Park H-J, Kim Y-K, Kim T-I (2015) Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; anin vitroandin vivostudy. J Periodontal Implant Sci 45:120. https://doi.org/10.5051/jpis.2015.45.3.120 CrossRefPubMedPubMedCentral

17.

Le Guéhennec L, Soueidan A, Layrolle P, Amouriq Y (2007) Surface treatments of titanium dental implants for rapid osseointegration. Dent Mater 23:844–854CrossRefPubMed

18.

Kato E, Sakurai K, Yamada M (2015) Periodontal-like gingival connective tissue attachment on titanium surface with nano-ordered spikes and pores created by alkali-heat treatment. Dent Mater 31:e116–e130. https://doi.org/10.1016/j.dental.2015.01.014 CrossRefPubMed

19.

Kearns VR, Williams RL, Mirvakily F, Doherty PJ, Martin N (2013) Guided gingival fibroblast attachment to titanium surfaces: an in vitro study. J Clin Periodontol 40:99–108. https://doi.org/10.1111/jcpe.12025 CrossRefPubMed

20.

Lee HJ, Lee J, Lee JT, Hong JS, Lim BS, Park HJ, Kim YK, Kim TI (2015) Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study. J Periodontal Implant Sci 45:120–126. https://doi.org/10.5051/jpis.2015.45.3.120 CrossRefPubMedPubMedCentral

21.

Chou L, Firth JD, Uitto VJ, Brunette DM (1995) Substratum surface topography alters cell shape and regulates fibronectin mRNA level, mRNA stability, secretion and assembly in human fibroblasts. J Cell Sci 108(Pt 4):1563–1573PubMed

22.

Eisenbarth E, Linez P, Biehl V, Velten D, Breme J, Hildebrand HF (2002) Cell orientation and cytoskeleton organisation on ground titanium surfaces. Biomol Eng 19:233–237CrossRefPubMed

23.

Oakley C, Brunette DM (1993) The sequence of alignment of microtubules, focal contacts and actin filaments in fibroblasts spreading on smooth and grooved titanium substrata. J Cell Sci 106(Pt 1):343–354PubMed

24.

Kronström M, Svensson B, Erickson E, Houston L, Braham P, Persson GR (2000) Humoral immunity host factors in subjects with failing or successful titanium dental implants. J Clin Periodontol 27:875–882CrossRefPubMed

25.

Satue M, Ramis JM, Monjo M (2015) Cholecalciferol synthesized after UV-activation of 7-dehydrocholesterol onto titanium implants inhibits osteoclastogenesis in vitro. J Biomed Mater Res A 103:2280–2288. https://doi.org/10.1002/jbm.a.35364 CrossRefPubMed

26.

Alfarsi MA, Hamlet SM, Ivanovski S (2014) The effect of platelet proteins released in response to titanium implant surfaces on macrophage pro-inflammatory cytokine gene expression. Clin Implant Dent Relat Res 17:1036–1047. https://doi.org/10.1111/cid.12231 CrossRefPubMed

27.

Hamlet S, Alfarsi M, George R, Ivanovski S (2012) The effect of hydrophilic titanium surface modification on macrophage inflammatory cytokine gene expression. Clin Oral Implants Res 23:584–590. https://doi.org/10.1111/j.1600-0501.2011.02325.x CrossRefPubMed

28.

Hotchkiss KM, Reddy GB, Hyzy SL, Schwartz Z, Boyan BD, Olivares-Navarrete R (2015) Titanium surface characteristics, including topography and wettability, alter macrophage activation. Acta Biomater 31:425–434. https://doi.org/10.1016/j.actbio.2015.12.003 CrossRefPubMedPubMedCentral

29.

Refai AK, Textor M, Brunette DM, Waterfield JD (2004) Effect of titanium surface topography on macrophage activation and secretion of proinflammatory cytokines and chemokines. J Biomed Mater Res A 70:194–205. https://doi.org/10.1002/jbm.a.30075 CrossRefPubMed

30.

Tan KS, Qian L, Rosado R, Flood PM, Cooper LF (2006) The role of titanium surface topography on J774A.1 macrophage inflammatory cytokines and nitric oxide production. Biomaterials 27:5170–5177. https://doi.org/10.1016/j.biomaterials.2006.05.002 CrossRefPubMed

31.

Thalji GN, Nares S, Cooper LF (2014) Early molecular assessment of osseointegration in humans. Clin Oral Implants Res 25:1273–1285. https://doi.org/10.1111/clr.12266 CrossRefPubMed

32.

Wang X, Wang Y, Bosshardt DD, Miron RJ, Zhang Y (2017) The role of macrophage polarization on fibroblast behavior-an in vitro investigation on titanium surfaces. Clin Oral Investig 22:847–857. https://doi.org/10.1007/s00784-017-2161-8 CrossRefPubMed

33.

Wei L, Ke J, Prasadam I, Miron RJ, Lin S, Xiao Y, Chang J, Wu C, Zhang Y (2014) A comparative study of Sr-incorporated mesoporous bioactive glass scaffolds for regeneration of osteopenic bone defects. Osteoporos Int 25:2089–2096. https://doi.org/10.1007/s00198-014-2735-0 CrossRefPubMed

34.

Anderson JM, Rodriguez A and Chang DT (2008) Foreign body reaction to biomaterials. Book title. Elsevier

35.

Thalji G, Cooper LF (2014) Molecular assessment of osseointegration in vitro: a review of current literature. Int J Oral Maxillofac Implants 29:e171–e199. https://doi.org/10.11607/jomi.te55 CrossRefPubMed

36.

Chehroudi B, Ghrebi S, Murakami H, Waterfield JD, Owen G, Brunette DM (2010) Bone formation on rough, but not polished, subcutaneously implanted Ti surfaces is preceded by macrophage accumulation. J Biomed Mater Res A 93:724–737PubMed

37.

Ehrenfest DMD, Coelho PG, Kang B-S, Sul Y-T, Albrektsson T (2010) Classification of osseointegrated implant surfaces: materials, chemistry and topography. Trends Biotechnol 28:198–206CrossRef

38.

Morra M, Cassinelli C, Bruzzone G, Carpi A, Santi GD, Giardino R and Fini M (2003) Surface chemistry effects of topographic modification of titanium dental implant surfaces: 1. Surface analysis. Int J Oral Maxillofac Implants 18:40–45

39.

Hotchkiss KM, Ayad NB, Hyzy SL, Boyan BD, Olivares-Navarrete R (2017) Dental implant surface chemistry and energy alter macrophage activation in vitro. Clin Oral Implants Res 28:414–423CrossRefPubMed

40.

Hotchkiss KM, Clark NM, Olivares-Navarrete R (2018) Macrophage response to hydrophilic biomaterials regulates MSC recruitment and T-helper cell populations. Biomaterials 182:202–215. https://doi.org/10.1016/j.biomaterials.2018.08.029 CrossRefPubMed

41.

Albrektsson T, Dahlin C, Jemt T, Sennerby L, Turri A, Wennerberg A (2014) Is marginal bone loss around oral implants the result of a provoked foreign body reaction? Clin Implant Dent Relat Res 16:155–165. https://doi.org/10.1111/cid.12142 CrossRefPubMed

42.

Trindade R, Albrektsson T, Tengvall P, Wennerberg A (2014) Foreign body reaction to biomaterials: on mechanisms for buildup and breakdown of osseointegration. Clin Implant Dent Relat Res 18:192–203. https://doi.org/10.1111/cid.12274 CrossRefPubMed

43.

Trindade R, Albrektsson T, Wennerberg A (2015) Current concepts for the biological basis of dental implants: foreign body equilibrium and osseointegration dynamics. Oral Maxillofac Surg Clin North Am 27:175–183. https://doi.org/10.1016/j.coms.2015.01.004 CrossRefPubMed

44.

Berglundh T, Gotfredsen K, Zitzmann NU, Lang NP, Lindhe J (2007) Spontaneous progression of ligature induced peri-implantitis at implants with different surface roughness: an experimental study in dogs. Clin Oral Implants Res 18:655–661CrossRefPubMed

45.

Rimondini L, Faré S, Brambilla E, Felloni A, Consonni C, Brossa F, Carrassi A (1997) The effect of surface roughness on early in vivo plaque colonization on titanium. J Periodontol 68:556–562CrossRefPubMed

46.

Derks J, Schaller D, Hakansson J, Wennstrom JL, Tomasi C, Berglundh T (2016) Effectiveness of implant therapy analyzed in a Swedish population: prevalence of peri-implantitis. J Dent Res 95:43–49. https://doi.org/10.1177/0022034515608832 CrossRefPubMed

47.

Tarnow DP (2016) Increasing prevalence of peri-implantitis: how will we manage? J Dent Res 95:7–8. https://doi.org/10.1177/0022034515616557 CrossRefPubMed

48.

Koldsland OC, Scheie AA, Aass AM (2010) Prevalence of peri-implantitis related to severity of the disease with different degrees of bone loss. J Periodontol 81:231–238. https://doi.org/10.1902/jop.2009.090269 CrossRefPubMed

49.

Mendonça G, Mendonça DBS, Aragao FJL, Cooper LF (2008) Advancing dental implant surface technology–from micron-to nanotopography. Biomaterials 29:3822–3835CrossRefPubMed

Titel: Macrophage behavior and interplay with gingival fibroblasts cultured on six commercially available titanium, zirconium, and titanium-zirconium dental implants
verfasst von: Yulan Wang
Yufeng Zhang
Anton Sculean
Dieter D Bosshardt
Richard J Miron
Publikationsdatum: 10.11.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 8/2019
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2736-z

Neu im Fachgebiet Zahnmedizin

19.04.2024 | Vorhofflimmern | Nachrichten

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Macrophage behavior and interplay with gingival fibroblasts cultured on six commercially available titanium, zirconium, and titanium-zirconium dental implants

Abstract

Objectives

Materials and methods

Results

Conclusions and clinical relevance

Neu im Fachgebiet Zahnmedizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

Die Oralprophylaxe & Kinderzahnheilkunde umbenannt

Newsletter

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Objectives

Materials and methods

Results

Conclusions and clinical relevance

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 8/2019

The fate of root canals obturated with Thermafil: 10-year data for patients treated in a master’s program

Influence of treatment duration on the efficacy of at-home bleaching with daytime application: a randomized clinical trial

Expression of growth mediators in the gingival crevicular fluid of patients with aggressive periodontitis undergoing periodontal surgery

Swallowing changes related to chronic temporomandibular disorders

Comparative study of articaine and lidocaine without palatal injection for maxillary teeth extraction

Influence of polycystic ovary syndrome on the periodontal health of Indian women visiting a secondary health care centre

Neu im Fachgebiet Zahnmedizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

Die Oralprophylaxe & Kinderzahnheilkunde umbenannt

Newsletter