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05.02.2019 | Original Article

Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14

verfasst von: Ya-Ying Chang, Tzu-Yu Lin, Ming-Chang Kao, Tsung-Ying Chen, Ching-Feng Cheng, Chih-Shung Wong, Chun-Jen Huang

Erschienen in: Inflammopharmacology | Ausgabe 2/2019

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Abstract

We investigated effects of magnesium sulfate (MgSO₄) on modulating lipopolysaccharide (LPS)–macrophage binding and cluster of differentiation 14 (CD14) expression. Flow cytometry data revealed that the mean levels of LPS-macrophage binding and membrane-bound CD14 expression (mCD14) in differentiated THP-1 cells (a human monocytic cell line) treated with LPS plus MgSO₄ (the LPS + M group) decreased by 28.2% and 25.3% compared with those THP-1 cells treated with LPS only (the LPS group) (P < 0.001 and P = 0.037), indicating that MgSO₄ significantly inhibits LPS–macrophage binding and mCD14 expression. Notably, these effects of MgSO₄ were counteracted by L-type calcium channel activation. Moreover, the mean level of soluble CD14 (sCD14; proteolytic cleavage product of CD14) in the LPS + M group was 25.6% higher than in the LPS group (P < 0.001), indicating that MgSO₄ significantly enhances CD14 proteolytic cleavage. Of note, serine protease inhibition mitigated effects of MgSO₄ on both decreasing mCD14 and increasing sCD14. In conclusion, MgSO₄ inhibits LPS–macrophage binding through reducing CD14 expression. The mechanisms may involve antagonizing L-type calcium channels and activating serine proteases.

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Titel: Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14
verfasst von: Ya-Ying Chang
Tzu-Yu Lin
Ming-Chang Kao
Tsung-Ying Chen
Ching-Feng Cheng
Chih-Shung Wong
Chun-Jen Huang
Publikationsdatum: 05.02.2019
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 2/2019
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-019-00568-7

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14

Abstract

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

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