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Archives of Gynecology and Obstetrics

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23.07.2018 | General Gynecology

Magnesium sulphate can alleviate oxidative stress and reduce inflammatory cytokines in rat placenta of intrahepatic cholestasis of pregnancy model

verfasst von: Fei Han, Linhao Xu, Yaqing Huang, Tianqi Chen, Tiancheng Zhou, Liwei Yang

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 3/2018

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Abstract

Purpose

In our study, we try to investigate whether magnesium sulphate (MgSO₄) could provide protection against oxidative damage and inflammatory response in rat placenta of intrahepatic cholestasis of pregnancy (ICP) model.

Methods

The rat model of ICP was established by injecting s.c. 17α-ethinyl estradiol (EE) daily for 5 days. MgSO₄, as an therapeutic drug for ICP, was injected i.p. daily for 3 days. Age-matched pregnant rats served as controls. The level of serum total bile acid (TBA) was measured. The data including the number and weight of offsprings on day 20 of pregnancy were collected. We observed ultrastructural changes of mitochondria and endoplasmic reticulum (ER) in placenta by transmission electron microscope. The antioxidant proteins peroxiredoxin-6 (Prdx6) and nuclear factor erythroid 2-related factor-2 (Nrf2) were analyzed by Western Blot. The inflammatory cytokines including IL-1β, TNF-α and IFN-γ were investigated by real-time PCR (RT-PCR) and enzyme-linked immune-sorbent assay (ELISA).

Results

The weight of offsprings on day 20 of pregnancy increased in ICP rats treated with MgSO₄ (ICP + MG group) compared with that in ICP rats (ICP group). However, the level of TBA was not reduced. The damage of mitochondria and ER was observed in placenta, which was much more slighter in ICP + MgSO₄ group as compared with that in ICP group. Prdx6 and Nrf2 were increased, while the inflammatory cytokines including IL-1β, TNF-α and IFN-γ were decreased in ICP + MgSO₄ group compared with that in ICP group.

Conclusions

MgSO₄ had beneficial effect on improving growth of offsprings in rat model of ICP. The protective effect of MgSO₄ on alleviating oxidative damage and inflammatory response in placenta may play an important role in the process. MgSO₄ may improve the function of placenta.

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Titel: Magnesium sulphate can alleviate oxidative stress and reduce inflammatory cytokines in rat placenta of intrahepatic cholestasis of pregnancy model
verfasst von: Fei Han
Linhao Xu
Yaqing Huang
Tianqi Chen
Tiancheng Zhou
Liwei Yang
Publikationsdatum: 23.07.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 3/2018
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-018-4850-1

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Magnesium sulphate can alleviate oxidative stress and reduce inflammatory cytokines in rat placenta of intrahepatic cholestasis of pregnancy model

Abstract

Purpose

Methods

Results

Conclusions

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