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Molecular Imaging and Biology

Erschienen in:

11.04.2019 | Brief Article

Mapping pH at Cancer Cell Surfaces

verfasst von: Da Wei, Donald M. Engelman, Yana K. Reshetnyak, Oleg A. Andreev

Erschienen in: Molecular Imaging and Biology | Ausgabe 6/2019

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Abstract

Purpose

To develop a tool to measure the pH at the surfaces of individual cells.

Procedures

The SNARF pH-sensitive dye was conjugated to a pHLIP® peptide (pH-Low Insertion Peptide) that binds cellular membranes in tumor spheroids. A beam splitter allows simultaneous recording of two images (580 and 640 nm) by a CCD camera. The ratio of the two images is converted into a pH map resolving single spheroid cells. An average pH for each cell is calculated and a pH histogram is derived.

Results

Surface pH depends on cellular glycolytic activity, which was varied by adding glucose or deoxy-glucose. Glucose was found to decrease the surface pH relative to the pH of the bulk solution. The surface pH of metastatic cancer cells was lower than that of non-metastatic cells indicating a higher glycolytic activity.

Conclusions

Our method allows cell surface pH measurement and its correlation with cellular glycolytic activity.

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Titel: Mapping pH at Cancer Cell Surfaces
verfasst von: Da Wei
Donald M. Engelman
Yana K. Reshetnyak
Oleg A. Andreev
Publikationsdatum: 11.04.2019
Verlag: Springer International Publishing
Erschienen in: Molecular Imaging and Biology / Ausgabe 6/2019
Print ISSN: 1536-1632
Elektronische ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-019-01335-4

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Mapping pH at Cancer Cell Surfaces

Abstract

Purpose

Procedures

Results

Conclusions

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Procedures

Results

Conclusions

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