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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Mass blood survey for malaria: pooling and real-time PCR combined with expert microscopy in north-west Thailand

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Kanungnit Congpuong, Aungkana SaeJeng, Rungniran Sug-aram, Supannee Aruncharus, Ampai Darakapong, Steven R Meshnick, Wichai Satimai
Wichtige Hinweise

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

AS, RS, SA and AD obtained the dried blood spots, extracted DNA, ran the PCR’s. KC and AS are expert microscopists and did blood examinations KC, SM and WS participated in analyzing the data and writing the manuscript. All authors read and approved the final manuscript

Abstract

Background

Asymptomatic carriage of Plasmodium falciparum and Plasmodium vivax is common in both low-and high-transmission settings and represents an important reservoir of infection that needs to be targeted if malaria elimination is to succeed.

Methods

Mass blood examinations (475 individuals) were conducted in two villages in Mae Hong Son, an area of endemic but low-transmission malaria in the north-west of Thailand. The microscopist at the local malaria clinic did not detect any infections. Pools of four samples were screened by real-time PCR; individual members of all of the positive pools were then re-examined by expert microscopy and by a second species-specific PCR reaction.

Results

Eight subjects were found to be positive by both PCR and expert microscopy and one was found to be positive by PCR alone. The slides contained asexual stage parasites of P. vivax, P. falciparum and Plasmodium malariae, but no gametocytes. The local clinic was notified within two to eight days of the survey.

Conclusion

A combination of pooling, real-time PCR and expert microscopy provides a feasible approach to identifying and treating asymptomatic malaria infections in a timely manner.
Literatur
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