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01.12.2014

Mast Cells Kill Candida albicans in the Extracellular Environment but Spare Ingested Fungi from Death

verfasst von: Elisa Trevisan, Francesca Vita, Nevenka Medic, Maria Rosa Soranzo, Giuliano Zabucchi, Violetta Borelli

Erschienen in: Inflammation | Ausgabe 6/2014

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Abstract

Mast cells (MCs) reside in tissues that are common targets of Candida spp. infections, and can exert bactericidal activity, but little is known about their fungicidal activity. MCs purified from rat peritoneum (RPMC) and a clinical isolate of C. albicans, were employed. Ingestion was evaluated by flow cytometry (FACS) and optical microscopy. The killing activity was assayed by FACS analysis and by colony forming unit method. RPMC degranulation was evaluated by β-hexosaminidase assay and phosphatidylserine externalization by FACS. Phagocytosing RPMC were also analyzed by transmission electron microscopy. Herein, we show that the killing of C. albicans by RPMC takes place in the extracellular environment, very likely through secreted granular components. Ultrastructural analysis of the ingestion process revealed an unusual RPMC–C. albicans interaction that could allow fungal survival. Our findings indicate that MCs have a positive role in the defense mechanism against Candida infections and should be included among the cell types involved in host-defense against this pathogen.

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Titel: Mast Cells Kill Candida albicans in the Extracellular Environment but Spare Ingested Fungi from Death
verfasst von: Elisa Trevisan
Francesca Vita
Nevenka Medic
Maria Rosa Soranzo
Giuliano Zabucchi
Violetta Borelli
Publikationsdatum: 01.12.2014
Verlag: Springer US
Erschienen in: Inflammation / Ausgabe 6/2014
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-014-9951-9

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Mast Cells Kill Candida albicans in the Extracellular Environment but Spare Ingested Fungi from Death

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