The oxysterol 27-hydroxycholesterol (27-OHC) plays an important role in the regulation of cholesterol homeostasis. Pregnancy pathologies like preeclampsia (PE), HELLP-syndrome (HELLP), intrauterine growth restriction (IUGR) and intrahepatic cholestasis in pregnancy (ICP) are linked to disturbances in lipid metabolism. In the present study, we hypothesized a specific gestational regulation of 27-OHC and compromised 27-OHC levels due to placental and hepatic diseases in pregnancy resulting in a dysregulation of lipid metabolism.
The 27-OHC was measured by gas-chromatography-mass spectrometry (GC-MS) and related to cholesterol concentrations. In the longitudinal cohort, a complete set of samples of healthy patients (n = 33) obtained at three different time points throughout gestation and once post-partum was analyzed. In the cross sectional cohort, patients with pregnancy pathologies (IUGR n = 14, PE n = 14, HELLP n = 7, ICP n = 7) were matched to a control group (CTRL) of equal gestational ages.
The 27-OHC levels already increased in the first trimester despite lower TC concentrations (p < 0.05). During the course of pregnancy, a subtle rise in 27-OHC concentrations results in an overall decrease of 27-OHC/TC ratio in between the first (p < 0.05) and second trimester. The ratio remains stable thereafter including the post-partum period. No significant differences have been observed in pregnancy pathologies as compared to the CTRL group.
In conclusion, 27-OHC may have a compensatory role in cholesterol metabolism early in pregnancy. The conserved 27-OHC/TC ratio in pregnancy pathologies suggest that neither the placenta nor the liver is majorly involved in the regulation of 27-OHC metabolism.
Dann AT, Kenyon AP, Wierzbicki AS, Seed PT, Shennan AH, Tribe RM. Plasma lipid profiles of women with intrahepatic cholestasis of pregnancy. Am J Obstet Gynecol. 2006;107(1):106–14. CrossRef
Babiker A, Andersson O, Lund E, Xiu RJ, Deeb S, Reshef A, et al. Elimination of cholesterol in macrophages and endothelial cells by the sterol 27-hydroxylase mechanism. Comparison with high density lipoprotein-mediated reverse cholesterol transport. Biol Chem. 1997;272(42):26253–61. CrossRef
Bjorkhem I. Mechanism of degradation of the steroid side chain in the formation of bile acids. Journal of lipid research 1992; 33(4):455–71. Reiss AB, Martin KO, Rojer DE, Iyer S, Grossi EA, Galloway AC et al. Sterol 27-hydroxylase: expression in human arterial endothelium. J Lipid Res. 1997;38(6):1254–60.
Mukhamedova N, Escher G, D’Souza W, Tchoua U, Grant A, Krozowski Z, et al. Enhancing apolipoprotein A-I-dependent cholesterol efflux elevates cholesterol export from macrophages in vivo. J Lipid Research. 2008;49(11):2312–22. CrossRef
Reiss AB, Martin KO, Rojer DE, Iyer S, Grossi EA, Galloway AC, et al. Sterol 27-hydroxylase: expression in human arterial endothelium. J Lipid Res. 1997;38(6):1254–60. PubMed
Martin KO, Reiss AB, Lathe R, Javitt NB. 7 alpha-hydroxylation of 27-hydroxycholesterol: biologic role in the regulation of cholesterol synthesis. J Lipid Res. 1997;38(5):1053–8. PubMed
American College of Obstetricians and Gynecologists (ACOG). Fetal growth restriction. Washington (DC: American College of Obstetricians and Gynecologists (ACOG); 2013. ACOG practice bulletin; no. 134.
Seiler E, Woitke AK, Schollberg K, Riehn A. Untersuchungen über den Zusammenhang von Progesteron und Apoprotein B in der Schwangerschaft [Correlation of progesterone and apoprotein B in pregnancy]. Zentralbl Gynakol. 1988;110(21):1340–4. PubMed
Sattar N, Greer IA, Galloway PJ, Packard CJ, Shepherd J, Kelly T, et al. Lipid and lipoprotein concentrations in pregnancies complicated by intrauterine growth restriction. J Clin Endocrinol Metab. 1999;84(1):128–30. PubMed
- Maternal 27-hydroxycholesterol concentrations during the course of pregnancy and in pregnancy pathologies
Brigitte Sophia Winkler
- BioMed Central
Neu im Fachgebiet Gynäkologie und Geburtshilfe
Meistgelesene Bücher aus dem Fachgebiet
Mail Icon II