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01.12.2017 | Research | Ausgabe 1/2017 Open Access

Chinese Medicine 1/2017

Mechanisms for the anti-obesity actions of bofutsushosan in high-fat diet-fed obese mice

Zeitschrift:
Chinese Medicine > Ausgabe 1/2017
Autoren:
Shinjiro Kobayashi, Yuki Kawasaki, Tatsuo Takahashi, Hironori Maeno, Masaaki Nomura
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s13020-017-0129-x) contains supplementary material, which is available to authorized users.

Abstract

Background

The Kampo medicine bofutsushosan (BTS; Pulvis ledebouriellae compositae; Fang Feng Tong Sheng San) has been used as an anti-obesity treatment in overweight patients. In this study, we assessed the underlying physiological changes induced by BTS in obese mice maintained on a high-fat diet.

Methods

Male ICR mice were fed a 60% kcal fat diet for 5 weeks starting at 4 weeks of age and then fed the same diet with administration of water (control) or aqueous BTS extract (1.0–2.0 g/kg) for 25 days. Body weight, wet weight of isolated white adipose tissue, and obesity-related serum parameters (glucose, lipids, leptin, adiponectin) were measured after treatment. The mRNA expression levels of leptin, adiponectin, and UCP1 in the adipose tissues were determined by quantitative real-time polymerase chain reaction after the first 5 days of treatment.

Results

Bofutsushosan (1.5–2.0 g/kg) significantly decreased total body weight and total wet weight of white adipose tissue isolated from subcutaneous (retroperitoneal) and visceral regions (epididymal, mesenteric, and perirenal). At 2.0 g/kg, BTS also decreased total fat mass, visceral fat mass, and ratio of fat mass to body weight as measured by computed tomography, and significantly decreased epididymal adipocyte size after 14 and 25 days’ treatment. Twenty-five days’ treatment lowered serum glucose, insulin, leptin, and triglycerides, and reduced homeostasis model assessment-insulin resistance. Alternatively, 2.0 g/kg BTS significantly increased mRNA levels of adiponectin, leptin, and UCP1 in interscapular brown adipose tissue but not epididymal white adipose tissue after 5 days’ administration.

Conclusion

In the early administration period, BTS increased mRNA expression levels of leptin, adiponectin, and UCP1 in brown adipose tissues. With longer administration, BTS improved insulin resistance, and subsequently reduced serum levels of leptin and triglyceride in parallel with decreased visceral white adipose tissue volume and adipocyte size.
Zusatzmaterial
Additional file 1. The ARRIVE Guidelines Checklist.
Additional file 2. Minimum standards of reporting checklist.
Additional file 3. Summary of all datasets in Figs. 1, 2, 3, 4, 5, 6 and 7, and Table 2.
Literatur
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