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Erschienen in: Endocrine 3/2016

08.04.2016 | Original Article

Metabolic actions of insulin in ovarian granulosa cells were unaffected by hyperandrogenism

verfasst von: Shidou Zhao, Haijing Xu, Yuqian Cui, Wenting Wang, Yingying Qin, Li You, Wai-Yee Chan, Yun Sun, Zi-Jiang Chen

Erschienen in: Endocrine | Ausgabe 3/2016

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Abstract

Polycystic ovary syndrome (PCOS) patients have intra-ovarian hyperandrogenism and granulosa cells (GCs) from PCOS patients have impaired insulin-dependent glucose metabolism and insulin resistance. The purpose of this study is to determine whether excess androgen affects glucose metabolism and induces insulin resistance of GCs. We firstly explored the insulin metabolic signaling pathway and glucose metabolism in cultured GCs. The Akt phosphorylation and lactate production were increased after insulin treatment. Pre-treatment with PI3-K inhibitor attenuated insulin-induced phosphorylation of Akt and lactate accumulation. However, after treating GCs with different concentrations of testosterone for 5 days, insulin-induced phosphorylation of Akt and lactate production showed no significant change comparing with those of control cells. Finally, mRNA expression of insulin signaling mediators including INSR, IRS-1, IRS-2, and GLUT-4 in GCs was also not significantly altered after testosterone treatment. In conclusion, insulin activates PI3-K/Akt signaling pathway and promotes lactate production in ovarian GCs, but high androgen exerted no obvious influence on insulin signaling pathway and metabolic effect in GCs, suggesting that metabolic actions of insulin in ovarian GCs were unaffected by hyperandrogenism directly.
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Metadaten
Titel
Metabolic actions of insulin in ovarian granulosa cells were unaffected by hyperandrogenism
verfasst von
Shidou Zhao
Haijing Xu
Yuqian Cui
Wenting Wang
Yingying Qin
Li You
Wai-Yee Chan
Yun Sun
Zi-Jiang Chen
Publikationsdatum
08.04.2016
Verlag
Springer US
Erschienen in
Endocrine / Ausgabe 3/2016
Print ISSN: 1355-008X
Elektronische ISSN: 1559-0100
DOI
https://doi.org/10.1007/s12020-016-0949-y

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