Background
Methods
Subjects and characteristics
Subject alias | Age | Gender | Tanner stage | Height (in cm) | Weight (in kg) | BMI | BMI-SDS |
---|---|---|---|---|---|---|---|
T1DM patients | |||||||
1 | 17.2 | 1 | 5 | 167.0 | 54.0 | 19.4 | −0.38 |
2 | 15.0 | 0 | 5 | 159.0 | 58.0 | 22.9 | 1.01 |
3 | 11.4 | 1 | 2 | 152.0 | 37.0 | 16.0 | −0.52 |
4 | 10.0 | 0 | 1 | 148.0 | 34.5 | 15.8 | −0.31 |
5 | 8.7 | 0 | 1 | 140.0 | 33.2 | 16.9 | 0.59 |
6 | 11.8 | 1 | 2 | 149.0 | 40.5 | 18.2 | 0.37 |
7 | 10.1 | 1 | 1 | 139.5 | 30.0 | 15.4 | −0.53 |
Controls | |||||||
1 | 15.0 | 1 | 5 | 172.8 | 58.9 | 19.7 | 0.23 |
2 | 9.9 | 1 | 1 | 139.0 | 30.3 | 15.7 | −0.38 |
3 | 11.6 | 1 | 1 | 147.0 | 42.2 | 19.5 | 0.92 |
4 | 12.6 | 0 | 2 | 158.7 | 40.9 | 16.2 | −0.81 |
5 | 12.5 | 0 | 3 | 151.9 | 41.0 | 17.8 | −0.11 |
6 | 17.8 | 0 | 5 | 168.4 | 60.1 | 21.2 | 0.14 |
7 | 15.2 | 1 | 4 | 179.1 | 64.1 | 20.0 | 0.32 |
Biochemical measurements
Sample collection, preparation and 1H-NMR analysis
Statistical analysis
Results
VAR | Spectral range (ppm) | Assigned metabolite | Model based on all 110 VARs (including glucose related VARs) | Model based on 95 VARs (excluding glucose related VARs) | ||
---|---|---|---|---|---|---|
p(corr)[1] | VIP ± cvSE | p(corr)[1] | VIP ± cvSE | |||
18 | 5.4300–5.2752 | –HC=CH– in fatty acid chain | 0.890 | 2.07 ± 0.71 | 0.787 | 2.77 ± 0.82 |
24 | 4.4100–4.3159 | C1
H and C3
H in glycerol backbone of triglycerides + OCH
2 of choline in sphingomyelin/phosphatidylcholine | 0.856 | 0.89 ± 0.47 | 0.740 | 1.19 ± 0.63 |
34 | 4.0310–4.0136 |
l-Serine | 0.809 | 0.48 ± 0.30 | 0.743 | 0.65 ± 0.40 |
35 | 4.0136–4.0010 |
l-Serine | 0.715 | 0.43 ± 0.26 | 0.669 | 0.57 ± 0.38 |
38 | 3.9590–3.8330 |
d-Glucose | −0.837 | 3.56 ± 0.24 | ||
41 | 3.7956–3.7820 |
d-Glucose | −0.815 | 0.83 ± 0.24 | ||
42 | 3.7820–3.7550 |
d-Glucose | −0.861 | 2.19 ± 0.12 | ||
43 | 3.7550–3.7390 |
d-Glucose | −0.825 | 1.66 ± 0.14 | ||
44 | 3.7390–3.7141 |
d-Glucose | −0.782 | 1.42 ± 0.19 | ||
51 | 3.5914–3.5649 |
d-Glucose | −0.901 | 1.44 ± 0.27 | ||
52 | 3.5649–3.5510 |
d-Glucose | −0.828 | 1.04 ± 0.19 | ||
54 | 3.5360–3.3980 |
d-Glucose | −0.836 | 4.63 ± 0.54 | ||
55 | 3.3980–3.3765 |
l-Tryptophan | 0.721 | 0.81 ± 0.28 | 0.870 | 1.08 ± 0.35 |
63 | 3.1090–3.0860 |
l-Cysteine | 0.754 | 0.57 ± 0.37 | 0.701 | 0.77 ± 0.54 |
91 | 2.1230–1.9720 | –CH
2–CH=CH– in fatty acid chain | 0.752 | 2.17 ± 0.44 | 0.734 | 2.91 ± 0.65 |
100 | 1.3450–1.2458 | CH3–(CH
2)n– in fatty acid chain | 0.747 | 4.04 ± 1.52 | 0.728 | 5.41 ± 1.75 |
110 | 0.9660–0.8000 | CH
3–(CH2)n– in fatty acid chain | 0.898 | 3.14 ± 0.73 | 0.735 | 4.21 ± 1.03 |
VAR | Spectral range (ppm) | Assigned metabolite | Relative concentration | Increased/decreased in T1DM | p value* | %-change | |
---|---|---|---|---|---|---|---|
T1DM | Controls | ||||||
18 | 5.4300–5.2752 | –HC=CH– in fatty acid chain | 21.95 ± 3.63 | 30.95 ± 4.00 | ↓ | 0.001 | −29.1 |
24 | 4.4100–4.3159 | C1
H and C3
H in glycerol backbone of triglycerides + OCH
2 of choline in sphingomyelin/phosphatidylcholine | 3.28 ± 1.16 | 5.09 ± 1.16 | ↓ | 0.005 | −35.6 |
34 | 4.0310–4.0136 |
l-Serine | 0.70 ± 0.26 | 1.21 ± 0.22 | ↓ | 0.003 | −42.1 |
35 | 4.0136–4.0010 |
l-Serine | 0.84 ± 0.28 | 1.29 ± 0.23 | ↓ | 0.008 | −34.9 |
38 | 3.9590–3.8330 |
d-Glucose | 91.99 ± 16.9 | 64.44 ± 6.58 | ↑ | 0.004 | 42.8 |
41 | 3.7956–3.7820 |
d-Glucose | 7.62 ± 0.60 | 6.18 ± 0.60 | ↑ | <0.001 | 23.3 |
42 | 3.7820–3.7550 |
d-Glucose | 30.58 ± 5.63 | 20.56 ± 1.95 | ↑ | 0.003 | 48.7 |
43 | 3.7550–3.7390 |
d-Glucose | 17.92 ± 3.83 | 11.85 ± 1.35 | ↑ | 0.005 | 51.2 |
44 | 3.7390–3.7141 |
d-Glucose | 17.76 ± 3.24 | 13.05 ± 1.49 | ↑ | 0.007 | 36.1 |
51 | 3.5914–3.5649 |
d-Glucose | 14.45 ± 2.19 | 10.20 ± 1.06 | ↑ | 0.002 | 41.7 |
52 | 3.5649–3.5510 |
d-Glucose | 5.84 ± 1.10 | 3.65 ± 0.50 | ↑ | 0.002 | 60.0 |
54 | 3.5360–3.3980 |
d-Glucose | 116.37 ± 30.40 | 69.27 ± 8.54 | ↑ | 0.005 | 68.0 |
55 | 3.3980–3.3765 |
l-Tryptophan | 0.70 ± 0.32 | 1.94 ± 0.41 | ↓ | <0.001 | −63.9 |
63 | 3.1090–3.0860 |
l-Cysteine | 2.28 ± 0.47 | 3.05 ± 0.39 | ↓ | 0.006 | −25.2 |
91 | 2.1230–1.9720 | –CH
2–CH=CH– in fatty acid chain | 53.13 ± 7.19 | 64.03 ± 3.76 | ↓ | 0.007 | −17.0 |
100 | 1.3450–1.2458 | CH3–(CH
2)n– in fatty acid chain | 117.69 ± 17.62 | 155.40 ± 22.01 | ↓ | 0.004 | −24.3 |
110 | 0.9660–0.8000 | CH
3–(CH2)n– in fatty acid chain | 89.10 ± 14.79 | 111.75 ± 7.83 | ↓ | 0.006 | −20.3 |