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Erschienen in: International Urogynecology Journal 5/2017

13.10.2016 | Original Article

Methodology for a vaginal and urinary microbiome study in women with mixed urinary incontinence

verfasst von: Yuko M. Komesu, Holly E. Richter, Darrell L. Dinwiddie, Nazema Y. Siddiqui, Vivian W. Sung, Emily S. Lukacz, Beri Ridgeway, Lily A. Arya, Halina M. Zyczynski, Rebecca G. Rogers, Marie Gantz

Erschienen in: International Urogynecology Journal | Ausgabe 5/2017

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Abstract

Introduction and hypothesis

We describe the rationale and methods of a study designed to compare vaginal and urinary microbiomes in women with mixed urinary incontinence (MUI) and similarly aged, asymptomatic controls.

Methods

This paper delineates the methodology of a supplementary microbiome study nested in an ongoing randomized controlled trial comparing a standardized perioperative behavioral/pelvic floor exercise intervention plus midurethral sling versus midurethral sling alone for MUI. Women in the parent study had at least “moderate bother” from urgency and stress urinary incontinence symptoms (SUI) on validated questionnaire and confirmed MUI on bladder diary. Controls had no incontinence symptoms. All participants underwent vaginal and urine collection for DNA analysis and conventional urine culture. Standardized protocols were designed, and a central lab received samples for subsequent polymerase chain reaction (PCR) amplification and sequencing of the bacterial16S ribosomal RNA (rRNA) gene. The composition of bacterial communities will be determined by dual amplicon sequencing of variable regions 1–3 and 4–6 from vaginal and urine specimens to compare the microbiome of patients with controls. Sample-size estimates determined that 126 MUI and 84 control participants were sufficient to detect a 20 % difference in predominant urinary genera, with 80 % power and 0.05 significance level.

Results

Specimen collection commenced January 2015 and finished April 2016. DNA was extracted and stored for subsequent evaluation.

Conclusions

Methods papers sharing information regarding development of genitourinary microbiome studies, particularly with control populations, are few. We describe the rigorous methodology developed for a novel urogenital microbiome study in women with MUI.
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Metadaten
Titel
Methodology for a vaginal and urinary microbiome study in women with mixed urinary incontinence
verfasst von
Yuko M. Komesu
Holly E. Richter
Darrell L. Dinwiddie
Nazema Y. Siddiqui
Vivian W. Sung
Emily S. Lukacz
Beri Ridgeway
Lily A. Arya
Halina M. Zyczynski
Rebecca G. Rogers
Marie Gantz
Publikationsdatum
13.10.2016
Verlag
Springer London
Erschienen in
International Urogynecology Journal / Ausgabe 5/2017
Print ISSN: 0937-3462
Elektronische ISSN: 1433-3023
DOI
https://doi.org/10.1007/s00192-016-3165-7

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