nach oben

Erschienen in:

29.12.2017 | Original Article

Methylphenidate disrupts cytoskeletal homeostasis and reduces membrane-associated lipid content in juvenile rat hippocampus

verfasst von: Felipe Schmitz, Paula Pierozan, Helena Biasibetti-Brendler, Fernanda Silva Ferreira, Fernanda dos Santos Petry, Vera Maria Treis Trindade, Regina Pessoa-Pureur, Angela T. S. Wyse

Erschienen in: Metabolic Brain Disease | Ausgabe 3/2018

Einloggen, um Zugang zu erhalten

Abstract

Although methylphenidate (MPH) is ubiquitously prescribed to children and adolescents, the consequences of chronic utilization of this psychostimulant are poorly understood. In this study, we investigated the effects of MPH on cytoskeletal homeostasis and lipid content in rat hippocampus. Wistar rats received intraperitoneal injections of MPH (2.0 mg/kg) or saline solution (controls), once a day, from the 15th to the 44th day of age. Results showed that MPH provoked hypophosphorylation of glial fibrillary acidic protein (GFAP) and reduced its immunocontent. Middle and high molecular weight neurofilament subunits (NF-M, NF-H) were hypophosphorylated by MPH on KSP repeat tail domains, while NFL, NFM and NFH immunocontents were not altered. MPH increased protein phosphatase 1 (PP1) and 2A (PP2A) immunocontents. MPH also decreased the total content of ganglioside and phospholipid, as well as the main brain gangliosides (GM1, GD1a, and GD1b) and the major brain phospholipids (sphingomyelin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylserine). Total cholesterol content was also reduced in the hippocampi of juvenile rats treated with MPH. These results provide evidence that disruptions of cytoskeletal and lipid homeostasis in hippocampus of juvenile rats are triggers by chronic MPH treatment and present a new basis for understanding the effects and consequences associated with chronic use of this psychostimulant during the development of the central nervous system.

Adibhatla RM, Hatcher JF (2007) Role of lipids in brain injury and diseases. Future Lipidol 2(4):403–422. https://doi.org/10.2217/17460875.2.4.403 CrossRefPubMedPubMedCentral

Akay AP, Kaya GÇ, Emiroglu NI, Aydin A, Monkul ES, Tasçi C et al (2006) Effects of long-term methylphenidate treatment: a pilot follow-up clinical and SPECT study. Prog Neuropsychopharmacol Biol Psychiatry 30:1219–1224CrossRefPubMed

Andersen SL (2005) Stimulants and the developing brain. Trends Pharmacol Sci 26(5):237–243. https://doi.org/10.1016/j.tips.2005.03.009 CrossRefPubMed

Andersen SL, Napierata L, Brenhouse HC, Sonntag KC (2008) Juvenile methyphenidate modulates reward-related behaviours and cerebral blood flow by decreasing cortical D3 receptors. Eur J Neurosci 27(11):2962–2972. https://doi.org/10.1111/j.1460-9568.2008.06254.x CrossRefPubMed

Andreazza AC, Frey BN, Valvassori SS, Zanotto C, Gomes KM, Comim CM, Cassini C, Stertz L, Ribeiro LC, Quevedo J, Kapczinski F, Berk M, Gonçalves CA (2007) DNA damage in rats after treatment with methylphenidate. Prog Neuro-Psychopharmacol Biol Psychiatry 31(6):1282–1288. https://doi.org/10.1016/j.pnpbp.2007.05.012 CrossRef

Banerjee PS, Aston J, Khundakar AA, Zetterström T (2009) Differential regulation of psychostimulant-induced gene expression of brain derived neurotrophic factor and the immediate-early gene Arc in the juvenile and adult brain. Eur J Neurosci 29(3):465–476. https://doi.org/10.1111/j.1460-9568.2008.06601.x CrossRefPubMed

Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234(3):466–468PubMed

Brownlees J, Yates A, Bajaj NP, Davis D, Anderton BH, Leigh PN, Shaw CE, Miller CC (2000) Phosphorylation of neurofilament heavy chain side-arms by stress activated protein kinase-1b/Jun N-terminal kinase-3. J Cell Sci 113:401–407PubMed

Chase T, Carrey N, Soo E, Wilkinson M (2007) Methylphenidate regulates activity cytoskeletal associated but not brain-derived neurotrophic factor gene expression in the developing rat striatum. Neuroscience 144(3):969–984. https://doi.org/10.1016/j.neuroscience.2006.10.035 CrossRefPubMed

Cornelius F, Habeck M, Kanai R, Toyoshima C, Karlish SJD (2015) General and specific lipid–protein interactions in Na, K-ATPase. Biochim Biophys Acta 1848(9):1729–1743. https://doi.org/10.1016/j.bbamem.2015.03.012 CrossRefPubMed

Cyr M, Beaulieu JM, Laakso A, Sotnikova TD, Yao WD, Bohn LM, Gainetdinov RR, Caron MG (2003) Sustained elevation of extracellular dopamine causes motor dysfunction and selective degeneration of striatal GABAergic neurons. Proc Natl Acad Sci U S A 100(19):11035–11040. https://doi.org/10.1073/pnas.1831768100 CrossRefPubMedPubMedCentral

Dafny N, Yang PB (2006) The role of age, genotype, sex, and route of acute and chronic administration of methylphenidate: a review of its locomotor effects. Brain Res Bull 68(6):393–405. https://doi.org/10.1016/j.brainresbull.2005.10.005 CrossRefPubMed

de Almeida LM, Funchal C, Pde Pelaez L, Pessutto FD, Loureiro SO, Vivian L, Wajner M, Pessoa-Pureur R (2003) Effect of propionic and methylmalonic acids on the in vitro phosphorylation of intermediate filaments from cerebral cortex of rats during development. Metab Brain Dis 18(3):207–219. https://doi.org/10.1023/A:1025555132675 CrossRefPubMed

de Mattos-Dutra A, Sampaio de Freitas M, Schröder N, Fogaça Lisboa CS, Pessoa-Pureur R, Wajner M (1997) In vitro phosphorylation of cytoskeletal proteins in the rat cerebral cortex is decreased by propionic acid. Exp Neurol 147(2):238–247. https://doi.org/10.1006/exnr.1997.6602 CrossRefPubMed

du Bois TM, Deng C, Huang XF (2005) Membrane phospholipid composition, alterations in neurotransmitter systems and schizophrenia. Prog Neuro-Psychopharmacol Biol Psychiatry 29(6):878–888. https://doi.org/10.1016/j.pnpbp.2005.04.034 CrossRef

Fagundes AO, Rezin GT, Zanette F, Grandi E, Assis LC, Dal-Pizzol F, Quevedo J, Streck EL (2007) Chronic administration of methylphenidate activates mitochondrial respiratory chain in brain of young rats. Int J Dev Neurosci 25(1):47–51. https://doi.org/10.1016/j.ijdevneu.2006.11.001 CrossRefPubMed

Fagundes AO, Aguiar MR, Aguiar CS, Scaini G, Sachet MU, Bernhardt NM, Rezin GT, Valvassori SS, Quevedo J, Streck EL (2010a) Effect of acute and chronic adminis tration of methylphenidate on mitochondrial respiratory chain in the brain of young rats. Neurochem Res 35(11):1675–1680. https://doi.org/10.1007/s11064-010-0229-9 CrossRefPubMed

Fagundes AO, Scaini G, Santos PM, Sachet MU, Bernhardt NM, Rezin GT, Valvassori SS, Schuck PF, Quevedo J, Streck EL (2010b) Inhibition of mitochondrial respiratory chain in the brain of adult rats after acute and chronic administration of methylphenidate. Neurochem Res 35(3):405–411. https://doi.org/10.1007/s11064-009-0069-7 CrossRefPubMed

Farooqui AA, Ong WY, Horrocks LA (2004) Biochemical aspects of neurodegeneration in human brain: involvement of neural membrane phospholipids and phospholipases A2. Neurochem Res 29(11):1961–1977. https://doi.org/10.1007/s11064-004-6871-3 CrossRefPubMed

Ferriero DM (2001) Oxidant mechanisms in neonatal hypoxia-ischemia. Dev Neurosci 23(3):198–202. https://doi.org/10.1159/000046143 CrossRefPubMed

Folch J, Lees M, Sloane-Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226(1):497–509PubMed

Fragoso ALR, Trindade VMT (2015) Resorcinol-HCl method adaption for N-acetyl neuraminic acid measurement in an aqueous medium, oral and poster communication. In: XXVII SALÃO DE INICIAÇÃO CIENTÍFICA DA UFRGS, Porto Alegre, RS, Brazil

Friede RL, Samorajski T (1970) Axon caliber related to neurofilaments and microtubules in sciatic nerve fibers of rats and mice. Anat Rec 167(4):379–387. https://doi.org/10.1002/ar.1091670402 CrossRefPubMed

Funchal C, de Almeida LM, Loureiro SO, Vivian L, de Lima PP, Dall Bello FP et al (2003) In vitro phosphorylation of cytoskeletal proteins from cerebral cortex of rats. Brain Res Prot 11(2):111–118. https://doi.org/10.1016/S1385-299X(03)00022-9 CrossRef

Funchal C, Zamoner A, dos Santos AQ, Loureiro SO, Wajner M, Pessoa-Pureur R (2005) Alpha-ketoisocaproic acid increases phosphorylation of intermediate filament proteins from rat cerebral cortex by mechanisms involving Ca2+ and cAMP. Neurochem Res 30(9):1139–1146. https://doi.org/10.1007/s11064-005-7709-3 CrossRefPubMed

Gerasimov MR, Franceschi M, Volkow ND, Gifford A, Gatley SJ, Marsteller D et al (2000) Comparison between intraperitoneal and oral methylphenidate administration: a microdialysis and locomotor activity study. J Pharmacol Exp Ther 295:51–57PubMed

Goldstein ME, Sternberger NH, Sternberger LA (1987) Phosphorylation protects neurofilaments against proteolysis. J Neuroimmunol 14(2):149–160. https://doi.org/10.1016/0165-5728(87)90049-X CrossRefPubMed

Gonçalves J, Baptista S, Silva AP (2014) Psychostimulants and brain dysfunction: a review of the relevant neurotoxic effects. Neuropharmacology 87:135–149. https://doi.org/10.1016/j.neuropharm.2014.01.006 CrossRefPubMed

Gronier B, Aston J, Liauzun C, Zetterström T (2010) Age-dependent effects of methylphenidate in the prefrontal cortex: evidence from electrophysiological and arc gene expression measurements. J Psychopharmacol 24(12):1819–1827. https://doi.org/10.1177/0269881109359100 CrossRefPubMed

Guidato S, Bajaj NP, Miller CC (1996) Cellular phosphorylation of neurofilament heavy-chain by cyclin-dependent kinase-5 masks the epitope for monoclonal antibody N52. Neurosci Lett 217(2-3):157–160. https://doi.org/10.1016/S0304-3940(96)13098-6 CrossRefPubMed

Hakansson K, Lindskog M, Pozzi L, Usiello A, Fisone G (2004) DARP-32 and modulation of cAMP signaling: involvement in motor control and levodopa induced dyskinesia. Parkinsonism Relat Disord 10(5):281–286. https://doi.org/10.1016/j.parkreldis.2004.02.010 CrossRefPubMed

Head BP, Patel HH, Insel PA (2014) Interaction of membrane/lipid rafts with the cytoskeleton: impact on signaling and function:membrane/lipid rafts, mediators of cytoskeletal arrangement and cell signaling. Biochim Biophys Acta 1838(2):532–545. https://doi.org/10.1016/j.bbamem.2013.07.018 CrossRefPubMed

Heimfarth L, Loureiro SO, Reis KP, de Lima BO, Zamboni F, Lacerda S, Soska AK, Wild L, da Rocha JB, Pessoa-Pureur R (2012) Diphenyl ditelluride induces hypophosphorylation of intermediate filaments through modulation of DARPP-32-dependent pathways in cerebral cortex of young rats. Arch Toxicol 86(2):217–230. https://doi.org/10.1007/s00204-011-0746-6 CrossRefPubMed

Johnston LD, O’Malley PM, Bachman JG, Schulenberg JE (2011a) Monitoring the future national survey results on drug use, 1975–2010. Volume II: college students and adults ages 19–50. The University of Michigan, Bethesda, pp 1–312. http://www.monitoringthefuture.org/pubs/monographs/mtf-vol2_2010.pdf

Johnston LD, O’Malley PM, Bachman JG, Schulenberg JE (2011b) Monitoring the future national survey results on drug use, 1975–2010. Volume I: secondary school students. The University of Michigan, Bethesda, pp 1–734. http://www.monitoringthefuture.org/pubs/monographs/mtfoverview2014.pdf

Kabara JJ (1975) Brain cholesterol XVIII: EFFECt of methylphenidate (Ritalin) on [U-14C] glucose and [2-3H] acetate incorporation. Proc Soc Exp Biol Med 150(2):525–528. https://doi.org/10.3181/00379727-150-39070 CrossRefPubMed

Kronemberg G, Ende G, Alm B, Deuschle M, Heuser I, Colla M (2008) Increased NAA and reduced choline levels in the anterior cingulum following chronic methylphenidate. Eur Arch Psychiatry Clin Neurosci 258(7):446–450. https://doi.org/10.1007/s00406-008-0810-2 CrossRef

Kuczenski R, Segal DS (2001) Locomotor effects of acute and repeated threshold doses of amphetamine and methylphenidate: relative roles of dopamine and norepinephrine. J Pharmacol Exp Ther 296(3):876–883PubMed

Kuczenski R, Segal DS (2002) Exposure of adolescent rats to oral methylphenidate: preferential effects on extracellular norepinephrine and absence of sensitization and cross-sensitization to methamphetamine. J Neurosci 22(16):7264–7271CrossRefPubMed

Kudryashov IE, Onufriev MV, Kudryashova IV, Gulyaeva NV (2001) Periods of postnatal maturation of hippocampus: synaptic modifications and neuronal disconnection. Dev Brain Res 132(2):113–120. https://doi.org/10.1016/S0165-3806(01)00301-7 CrossRef

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685. https://doi.org/10.1038/227680a0 CrossRefPubMed

Lagace DC, Yee JK, Bolanos CA, Eisch AJ (2006) Juvenile administration of methylphenidate attenuates adult hippocampal neurogenesis. Biol Psychiatry 60(10):1121–1130. https://doi.org/10.1016/j.biopsych.2006.04.009 CrossRefPubMed

Lake BD, Goodwin HJ (1976) Lipids. In: Smith I, Seakings JWT (eds) Chromatographic and Eletrophoretic techniques, Paper and thin layer chromatography, vol 1. William Heinemann Medical Books Ltd, London, pp 345–366

Lee SB, Bagley JA, Lee HY, Jan LY, Jan YN (2011) Pathogenic polyglutamine proteins cause dendrite defects associated with specific actin cytoskeletal alterations in drosophila. Proc Natl Acad Sci U S A 108(40):16795–16800. https://doi.org/10.1073/pnas.1113573108 CrossRefPubMedPubMedCentral

Loureiro SO, Romão L, Alves T, Fonseca A, Heimfarth L, Neto VM, Wyse AT, Pessoa-Pureur R (2010) Homocysteine induces cytoskeletal remodeling and production of reactive oxygen species in cultured cortical astrocytes. Brain Res 1355:151–164. https://doi.org/10.1016/j.brainres.2010.07.071 CrossRefPubMed

Loureiro-Vieira S, Costa VM, de Lourdes Bastos M, Carvalho F, Capela JP (2017) Methylphenidate effects in the young brain: friend or foe? Int J Dev Neurosci 60:34–47. https://doi.org/10.1016/j.ijdevneu.2017.04.002 CrossRefPubMed

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–275PubMed

Middeldorp J, Hol EM (2011) GFAP in health and disease. Prog Neurobiol 93(3):421–443. https://doi.org/10.1016/j.pneurobio.2011.01.005 CrossRefPubMed

Mocchetti I (2005) Exogenous gangliosides, neuronal plasticity and repair, and the neurotrophins. Cell Mol Life Sci 62(19-20):2283–2294. https://doi.org/10.1007/s00018-005-5188-y CrossRefPubMed

Motaghinejad M, Motevalian M, Shabab B (2016) Effects of chronic treatment with methylphenidate on oxidative stress and inflammation in hippocampus of adult rats. Neurosci Lett 619:106–113. https://doi.org/10.1016/j.neulet.2015.12.015 CrossRefPubMed

Nakamura K, Handa S (1984) Coomassie brilliant blue staining of lipids on thin-layer plates. Anal Biochem 142(2):406–410. https://doi.org/10.1016/0003-2697(84)90484-6 CrossRefPubMed

Nores GA, Mitzumari RK, Kremer DM (1994) Chromatographic tank designed to obtain highly reproducible high-performance thin-layer chromatograms of gangliosides and neutral glycosphingolipids. J Chromatogr 686(1):155–157. https://doi.org/10.1016/S0021-9673(94)89015-3 CrossRef

Nyakas C, Buwalda B, Luiten PG (1996) Hypoxia and brain development. Prog Neurobiol 49(1):1–51. https://doi.org/10.1016/0301-0082(96)00007-X CrossRefPubMed

Ohvo-Rekilä H, Ramstedt B, Leppimäki P, Slotte JP (2002) Cholesterol interactions with phospholipids in membranes. Prog Lipid Res 41(1):66–97. https://doi.org/10.1016/S0163-7827(01)00020-0 CrossRefPubMed

Omary MB, Ku NO, Tao GZ, Toivola DM, Liao J (2006) “Heads and tails” of intermediate filamento phosphorylation: multiple sites and functional insights. Trends Biochem Sci 31(7):383–394. https://doi.org/10.1016/j.tibs.2006.05.008 CrossRefPubMed

Pant HC (1988) Dephosphorylation of neurofilament proteins enhances their susceptibility to degradation by calpain. Biochem J 256(2):665–668. https://doi.org/10.1042/bj2560665 CrossRefPubMedPubMedCentral

Pekny M, Pekna M (2004) Astrocyte intermediate filaments in CNS pathologies and regeneration. J Pathol 204(4):428–437. https://doi.org/10.1002/path.1645 CrossRefPubMed

Pfrieger FW (2003) Cholesterol homeostasis and function in neurons of the central nervous system. Cell Mol Life Sci 60:1158–1171CrossRefPubMed

Phan NT, Fletcher JS, Ewing AG (2015) Lipid structural effects of oral administration of methylphenidate in drosophila brain by secondary ion mass spectrometry imaging. Anal Chem 87(8):4063–4071. https://doi.org/10.1021/acs.analchem.5b00555 CrossRefPubMed

Pierozan P, Zamoner A, Soska AK, de Lima BO, Reis KP, Zamboni F, Wajner M, Pessoa-Pureur R (2012) Signaling mechanisms downstream of quinolinic acid targeting the cytoskeleton of rat striatal neurons and astrocytes. Exp Neurol 233(1):391–399. https://doi.org/10.1016/j.expneurol.2011.11.005 CrossRefPubMed

Pierozan P, Gonçalves CF, Ferreira F, Pessoa-Pureur R (2014) Acute intrastriatal injection of quinolinic acid provokes long-lasting misregulation of the cytoskeleton in the striatum, cerebral cortex and hippocampus of young rats. Brain Res 1577:1–10. https://doi.org/10.1016/j.brainres.2014.06.024 CrossRefPubMed

Quansah E, Sgamma T, Jaddoa E, Zetterström TSC (2017a) Chronic methylphenidate regulates genes and proteins mediating neuroplasticity in the juvenil erat brain. Neurosci Lett 654:93–98. https://doi.org/10.1016/j.neulet.2017.06.012 CrossRefPubMed

Quansah E, Ruiz-Rodado V, Grootveld M, Probert F, Zetterström TSC (2017b) 1H NMR-based metabolomics reveals neurochemical alterations in the brain of adolescent rats following acute methylphenidate administration. Neurochem Int 108:109–120. https://doi.org/10.1016/j.neuint.2017.03.003 CrossRefPubMed

Ramirez MR, Muraro F, Zylbersztejn DS, Abel CR, Arteni NS, Lavinsky D, Netto CA, Trindade VMT (2003) Neonatal hypoxiaischemia reduces ganglioside, phospholipid and cholesterol contents in the rat hippocampus. Neurosci Res 46(3):339–347. https://doi.org/10.1016/S0168-0102(03)00100-7 CrossRefPubMed

Réus GZ, Scaini G, Furlanetto CB, Morais MO, Jeremias IC, Mello Santos LM et al (2013) Methylphenidate treatment leads to abnor malities on Krebs cycle enzymes in the brain of young and adult rats. Neurotox Res 24(2):251–257. https://doi.org/10.1007/s12640-013-9383-6 CrossRefPubMed

Réus GZ, Scaini G, Titus SE, Furlanetto CB, Wessler LB, Ferreira GK, Gonçalves CL, Jeremias GC, Quevedo J, Streck EL (2015) Methylphenidate increases glucose uptake in the brain of young and adult rats. Pharmacol Rep 67(5):1033–1040. https://doi.org/10.1016/j.pharep.2015.03.005 CrossRefPubMed

Rice D, Barone S Jr (2000) Critical periods of vulnerability for the developing nervous system: evidence from humans and animal models. Environ Health Perspect 108(s3):511–533. https://doi.org/10.1289/ehp.00108s3511 CrossRefPubMedPubMedCentral

Röschlau P, Bernt E, Gruber W (1974) Cholesterol and esterified cholesterol, in: Bergmeyer, H.U., Gawehn, K. (Eds.), Methods of Enzymatic Analysis (Second English Edition): Academic, New York, pp. 1890-1893

Sadasivan S, Pond BB, Pani AK, Qu C, Jiao Y, Smeyne RJ (2012) Methylphenidate exposure induces dopamine neuron loss and activation of microglia in the basal ganglia of mice. PLoS One 7(3):e33693. https://doi.org/10.1371/journal.pone.0033693 CrossRefPubMedPubMedCentral

Schmitz F, Scherer EB, Machado FR, da Cunha AA, Tagliari B, Netto A, Wyse AT (2012a) Methylphenidate induces lipid and protein damage in prefrontal cortex, but not in cerebellum, striatum, and hippocampus of juvenile rats. Metab Brain Dis 27(4):605–612. https://doi.org/10.1007/s11011-012-9335-5 CrossRefPubMed

Schmitz F, Scherer EB, da Cunha MJ, da Cunha AA, Lima DD, Delwing D et al (2012b) Chronic methylphenidate administration alters antioxidant defenses and butyrylcholinesterase activity in blood of juvenile rats. Mol Cell Biochem 36:281–288CrossRef

Schmitz F, Pierozan P, Rodrigues AF, Biasibetti H, Grunevald M, Pettenuzzo LF, Scaini G, Streck EL, Netto CA, Wyse ATS (2016a) Methylphenidate causes behavioral impairments and neuron and astrocyte loss in the hippocampus of juvenile rats. Mol Neurobiol 54(6):4201–4216. https://doi.org/10.1007/s12035-016-9987-y CrossRefPubMed

Schmitz F, Pierozan P, Rodrigues AF, Biasibetti H, Grings M, Zanotto B, Coelho DM, Vargas CR, Leipnitz G, Wyse ATS (2016b) Methylphenidate decreases ATP levels and impairs glutamate uptake and Na⁺,K⁺-ATPase activity in juvenile rat hippocampus. Mol Neurobiol 54(10):7796–7807. https://doi.org/10.1007/s12035-016-0289-1 CrossRefPubMed

Schmitz F, Pierozan P, Rodrigues AF, Biasibetti H, Coelho DM, Mussulini BH, Pereira MS, Parisi MM, Barbé-Tuana F, Oliveira DL, Vargas CR, Wyse AT (2016c) Chronic treatment with a clinically relevant dose of methylphenidate increases glutamate levels in cerebrospinal fluid and impairs glutamatergic homeostasis in prefrontal cortex of juvenile rats. Mol Neurobiol 53:2385–2396

Schneider JS, Roeltgen DP, Mancall EL, Chapas-Crilly J, Rothblat DS, Tatarian GT (1998) Parkinson’s disease. Improved function with GM1 ganglioside treatment in a randomized placebo-controlled study. Neurology 50(6):1630–1636. https://doi.org/10.1212/WNL.50.6.1630 CrossRefPubMed

She JQ, Wang M, Zhu DM, Sun LG, Ruan DY (2005) Effect of ganglioside on synaptic plasticity of hippocampus in lead-exposed rats in vivo. Brain Res 1060(1-2):162–116. https://doi.org/10.1016/j.brainres.2005.08.044 CrossRefPubMed

Sihag RK, Inagaki M, Yamaguchi T, Shea TB, Pant HC (2007) Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments. Exp Cell Res 313(10):2098–2109. https://doi.org/10.1016/j.yexcr.2007.04.010 CrossRefPubMedPubMedCentral

Souza RP, Soares EC, Rosa DV, Souza BR, Gomes KM, Valvassori SS, Réus GZ, Inácio CG, Martins MR, Gomez MV, Quevedo J, Romano-Silva MA (2009) Cerebral DARPP-32 expression after methylphenidate administration in young and adult rats. Int J Dev Neurosci 27(1):1–7. https://doi.org/10.1016/j.ijdevneu.2008.11.001 CrossRefPubMed

Sowell ER, Peterson BS, Thompson PM, Welcome SE, Henkenius AL, Toga AW (2003) Mapping cortical change across the human life spa. Nat Neurosci 6(3):309–315. https://doi.org/10.1038/nn1008 CrossRefPubMed

Strack S, Westphal RS, Colbran RJ, Ebner FF, Wadzinski BE (1997) Protein serine/threonine phosphatase 1 and 2A associate with and dephosphorylate neurofilaments. Brain Res Mol Brain Res 49(1-2):15–28. https://doi.org/10.1016/S0169-328X(97)00117-4 CrossRefPubMed

Svennerholm L (1957) Quantitative estimation of sialic acids a colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta 24(3):604–611. https://doi.org/10.1016/0006-3002(57)90254-8 CrossRefPubMed

Svennerholm L (1963) Chromathographic separation of human brain gangliosides. J Neurochem 10(9):613–623. https://doi.org/10.1111/j.1471-4159.1963.tb08933.x CrossRefPubMed

Svenningsson P, Tzavara ET, Carruthers R, Rachleff I, Wattler S, Nehls M, McKinzie DL, Fienberg AA, Nomikos GG, Greengard P (2003) Diverse psychotomimetics act through a common signaling pathway. Science 302(5649):1412–1415. https://doi.org/10.1126/science.1089681 CrossRefPubMed

Trindade VMT, Perry ML, Bernard EA (1992) Gangliosides and sialoproteins in hypothalamus of normal, postnatal, and pre-and postnatal protein undernourished rats. J Neurol Sci 107(1):93–97. https://doi.org/10.1016/0022-510X(92)90214-6 CrossRefPubMed

Trindade VMT, Brusque AM, Raasch JR, Pettenuzzo LE, Rocha HP, Wannmacher CM, Wajner M (2002) Ganglioside alterations in the central nervous system of rats chronically injected with methylmalonic and propionic acids. Metab Brain Dis 17(2):93–102. https://doi.org/10.1023/A:1015464028616 CrossRefPubMed

Vannucci RC (1990) Experimental biology of hypoxia−/ischemia: relation to perinatal brain damage. Pediatr Res 27(4):317–356. https://doi.org/10.1203/00006450-199004000-00001 CrossRefPubMed

Veeranna GJ, Amin ND, Ahn NG, Jaffe H, Winters CA, Grant P, Pant HC (1998) Mitogen-activated protein kinases (Erk1,2) phosphorylate lys-ser-pro (KSP) repeats in neurofilament proteins NF-H and NF-M. J Neurosci 18(11):4008–4021CrossRefPubMed

Volkow ND, Wang GJ, Fowler JS, Logan J, Gerasimov M, Maynard L, Ding Y, Gatley SJ, Gifford A, Franseschi D (2001) Therapeutic doses of oral methylphenidate significantly increase extracellular dopamine in the human brain. J Neurosci 21:1–5CrossRef

Zanatta L, Goulart PB, Gonçalves R, Pierozan P, Winkelmann-Duarte EC, Woehl VM, Pessoa-Pureur R, Silva FR, Zamoner A (2012) 1α,25-dihydroxyvitamin D(3) mechanism of action: modulation of L-type calcium channels leading to calcium uptake and intermediate filament phosphorylation in cerebral cortex of young rats. Biochim Biophys Acta 1823(10):1708–1719. https://doi.org/10.1016/j.bbamcr.2012.06.023 CrossRefPubMed

Zhu Q, Couillard-Després S, Julien JP (1997) Delayed maturation of regenerating myelinated axons in mice lacking neurofilaments. Exp Neurol 148(1):299–316. https://doi.org/10.1006/exnr.1997.6654 CrossRefPubMed

Zito JM, Safer DJ, dos Reis S, Gardner JF, Boles M, Lynch F (2000) Trends in the prescribing of psychotropic medications to preschoolers. JAMA 283(8):1025–1030. https://doi.org/10.1001/jama.283.8.1025 CrossRefPubMed

Titel: Methylphenidate disrupts cytoskeletal homeostasis and reduces membrane-associated lipid content in juvenile rat hippocampus
verfasst von: Felipe Schmitz
Paula Pierozan
Helena Biasibetti-Brendler
Fernanda Silva Ferreira
Fernanda dos Santos Petry
Vera Maria Treis Trindade
Regina Pessoa-Pureur
Angela T. S. Wyse
Publikationsdatum: 29.12.2017
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 3/2018
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-017-0177-z

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

23.04.2024 | Demenz | Nachrichten

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Methylphenidate disrupts cytoskeletal homeostasis and reduces membrane-associated lipid content in juvenile rat hippocampus

Abstract

Leitlinien kompakt für die Neurologie

Neu im Fachgebiet Neurologie

Demenzkranke durch Antipsychotika vielfach gefährdet

Parkinson-Therapie im Wandel – aktuelle Leitlinie im Fokus

Auf diese Krankheiten bei Geflüchteten sollten Sie vorbereitet sein

Hustenstiller lindert Agitation bei Alzheimer

Update Neurologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2018

The effects of vitamin E on brain derived neurotrophic factor, tissues oxidative damage and learning and memory of juvenile hypothyroid rats

Turkish case of ethylmalonic encephalopathy misdiagnosed as short chain acyl-CoA dehydrogenase deficiency

Idalopirdine, a selective 5-HT6 receptor antagonist, reduces food intake and body weight in a model of excessive eating

Association between PDE4D rs966221 polymorphism and risk of ischemic stroke: a systematic review and meta-analysis

The effects of tramadol administration on hippocampal cell apoptosis, learning and memory in adult rats and neuroprotective effects of crocin

The possible factors affecting microglial activation in cases of obesity with cognitive dysfunction

Leitlinien kompakt für die Neurologie

Neu im Fachgebiet Neurologie

Demenzkranke durch Antipsychotika vielfach gefährdet

Parkinson-Therapie im Wandel – aktuelle Leitlinie im Fokus

Auf diese Krankheiten bei Geflüchteten sollten Sie vorbereitet sein

Hustenstiller lindert Agitation bei Alzheimer

Update Neurologie