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Erschienen in: Journal of Assisted Reproduction and Genetics 10/2018

09.07.2018 | REPRODUCTIVE PHYSIOLOGY AND DISEASE

Micro-RNAs involved in cellular proliferation have altered expression profiles in granulosa of young women with diminished ovarian reserve

verfasst von: Irene Woo, Lane K. Christenson, Sumedha Gunewardena, Sue Ann Ingles, Semara Thomas, Ali Ahmady, Karine Chung, Kristin Bendikson, Richard Paulson, Lynda K. McGinnis

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 10/2018

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Abstract

Purpose

The study aims to determine differences in micro-RNA (miRNA) expression in granulosa (GC) and cumulus cells (CC) between young women with diminished ovarian reserve (DOR) or normal ovarian reserve (NOR). Secondary objective was to identify downstream signaling pathways that could ultimately indicate causes of lower developmental competence of oocytes from young women with DOR.

Methods

The method of the study is prospective cohort study.

Results

Of the miRNA, 125 are differentially expressed in GC between DOR and NOR. Only nine miRNA were different in CC; therefore, we focused analysis on GC. In DOR GC, miR-100-5p, miR-16-5p, miR-30a-3p, and miR-193a-3p were significantly downregulated, while miR-155-5p, miR-192-5p, miR-128-3p, miR-486-5p, miR130a-3p, miR-92a-3p, miR-17-3p, miR-221-3p, and miR-175p were increased. This pattern predicted higher cell proliferation in the DOR GC. The primary pathways include MAPK, Wnt, and TGFbeta.

Conclusions

The miRNA pattern identified critical functions in cell proliferation and survival associated with DOR. GC in women with DOR seems to respond differently to the LH surge.
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Metadaten
Titel
Micro-RNAs involved in cellular proliferation have altered expression profiles in granulosa of young women with diminished ovarian reserve
verfasst von
Irene Woo
Lane K. Christenson
Sumedha Gunewardena
Sue Ann Ingles
Semara Thomas
Ali Ahmady
Karine Chung
Kristin Bendikson
Richard Paulson
Lynda K. McGinnis
Publikationsdatum
09.07.2018
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 10/2018
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-018-1239-9

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