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26.11.2021 | Original Article

Microbiome differences in periodontal, peri-implant, and healthy sites: a cross-sectional pilot study

verfasst von: Giovanni Barbagallo, Maria Santagati, Alaa Guni, Paolo Torrisi, Ambra Spitale, Stefania Stefani, Sebastiano Ferlito, Luigi Nibali

Erschienen in: Clinical Oral Investigations | Ausgabe 3/2022

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Abstract

Objectives

To explore microbial communities associated with health and disease status around teeth and dental implants.

Materials and methods

A total of 10 healthy, 24 periodontitis, and 24 peri-implant sites from 24 patients were sequenced by next-generation sequencing. Microbial DNA was extracted and 16S rRNA gene was amplified. Bioinformatic analyses were performed using quantitative insights into microbial ecology (QIIME), linear discriminant analysis effect size (LEfSE), and STAMP.

Results

Differences in microbial diversity across three types of sites were not statistically significant. Several genera and species were more prevalent in healthy compared with diseased sites, including Lautropia, Rothia and Capnocytophaga and Kingella. Among diseased sites, Peptostreptococcaceae, Dialister, Mongibacterium, Atopobium, and Filifactor were over-represented in peri-implantitis sites, while Bacteroidales was more abundant in periodontitis sites.

Conclusions

Diseased periodontal and peri-implant sites and corresponding healthy sites have distinct microbiological profiles. These findings suggest that microbial analyses could identify biomarkers for periodontal health and disease and lead to the development of new strategies to improve periodontal health and treat peri-implant and periodontal diseases.

Clinical relevance

The study contributes to improving our understanding of healthy, periodontally affected, and peri-implantitis sites which can improve our ability to diagnose, monitor, and manage these oral conditions.

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Titel: Microbiome differences in periodontal, peri-implant, and healthy sites: a cross-sectional pilot study
verfasst von: Giovanni Barbagallo
Maria Santagati
Alaa Guni
Paolo Torrisi
Ambra Spitale
Stefania Stefani
Sebastiano Ferlito
Luigi Nibali
Publikationsdatum: 26.11.2021
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 3/2022
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-021-04253-4

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Microbiome differences in periodontal, peri-implant, and healthy sites: a cross-sectional pilot study