Erschienen in:
14.12.2018 | Bone and Cartilage
MicroRNA-124 inhibits TNF-α- and IL-6-induced osteoclastogenesis
verfasst von:
Kenichiro Ohnuma, Shimpei Kasagi, Kenichi Uto, Yoriko Noguchi, Yuji Nakamachi, Jun Saegusa, Seiji Kawano
Erschienen in:
Rheumatology International
|
Ausgabe 4/2019
Einloggen, um Zugang zu erhalten
Abstract
Receptor activator for nuclear factor κB ligand (RANKL)-independent osteoclastogenic pathway was reported recently. MicroRNA (miR)-124 has been known to suppress RANKL-dependent osteoclastogenesis by inhibiting NFATc1 expression. However, whether miR-124 regulates a RANKL-independent pathway has not been elucidated. In this study, we examined whether a RANKL-independent pathway is regulated by miR-124 in addition to the RANKL-dependent one. Using osteoclastogenic culture and pit-formation assay, we found that a miR-124 mimic inhibited osteoclastogenesis in mouse bone marrow-derived macrophages stimulated by TNF-α, IL-6, and M-CSF in the presence of osteoprotegerin. We also showed that the expression levels of osteoclast-specific genes and NFATc1 protein were suppressed in the miR-124 mimic-transfected cells by performing quantitative-polymerase chain reaction and western blotting. Our results indicate that miR-124 is important in inhibiting both RANKL-dependent and -independent osteoclast differentiation by suppressing NFATc1-mediated pathway.