MicroRNA-125b regulates Th17/Treg cell differentiation and is associated with juvenile idiopathic arthritis
- 17.05.2019
- Original Article
Erschienen in:
- Verfasst von
- Zhi-Dan Fan
- Qian Cao
- Na Huang
- Le Ma
- Hui-Hui Ma
- Ya-Yuan Zhang
- Hai-Guo Yu
- Guo-Ping Zhou
- Erschienen in
- World Journal of Pediatrics | Ausgabe 1/2020
Abstract
Background
Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in childhood driven by aberrant pathways of T-cell activation. T helper 17 (Th17)/regulatory T cell (Treg) imbalance plays critical roles in the pathogenesis of arthritis. MicroRNA-125b (miR-125b) was upregulated after the activation of the initial CD4+ T cells, and could regulate the differentiation of CD4+ T cells. However, the effects of miR-125b on Th17/Treg imbalance and differentiation of Th17/Treg cells remain unknown.
Methods
In this study, we evaluated the expression of miR-125b in the peripheral blood mononuclear cells (PBMCs) of children with JIA, and the relationship of miR-125b with Th17/Treg imbalance. Then, we used lentivirus vector-mediated overexpression technology to investigate the regulatory function of miR-125b in CD4+ T cells or dendritic cell/CD4+ T co-culture system.
Results
Decreased miR-125b expression in PBMCs and CD4+ T cells of JIA patients was negatively correlated with the ratio of Th17/Treg cells. It also correlated negatively with retinoic acid receptor-related orphan receptor γt but positively with Forkhead box protein 3 at transcriptional levels. Furthermore, we found that miR-125b overexpression inhibited Th17 cell differentiation, whereas facilitated the differentiation of Treg cells. MiR-125b upregulation led to the decrease of Th17-secreting cytokines but the increase of the Treg-secreting cytokines.
Conclusions
Our results demonstrate that miR-125b participated in regulating Th17/Treg cell differentiation and imbalance in JIA patients. These findings provide novel insight into the critical role of miR-125b in the Th17/Treg imbalance of JIA, and raise the distinct possibility that miR-125b may prove to be a potential therapeutic target for JIA.
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- Titel
- MicroRNA-125b regulates Th17/Treg cell differentiation and is associated with juvenile idiopathic arthritis
- Verfasst von
-
Zhi-Dan Fan
Qian Cao
Na Huang
Le Ma
Hui-Hui Ma
Ya-Yuan Zhang
Hai-Guo Yu
Guo-Ping Zhou
- Publikationsdatum
- 17.05.2019
- Verlag
- Springer Singapore
- Erschienen in
-
World Journal of Pediatrics / Ausgabe 1/2020
Print ISSN: 1708-8569
Elektronische ISSN: 1867-0687 - DOI
- https://doi.org/10.1007/s12519-019-00265-z
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