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Erschienen in: Inflammation 3/2020

01.06.2020 | Original Article

MicroRNA-486-5p Promotes Acute Lung Injury via Inducing Inflammation and Apoptosis by Targeting OTUD7B

verfasst von: Qiang Luo, Jun Zhu, Qian Zhang, Jie Xie, Chengla Yi, Tianyu Li

Erschienen in: Inflammation | Ausgabe 3/2020

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Abstract

The aim of this article is to study the effect of miR-486-5p in acute lung injury (ALI). MiR-486-5p expression in peripheral blood was determined in ALI patients and healthy volunteers by qRT-PCR. ALI mouse model were reproduced by LPS treatment, and miR-486-5p NC and miRNA-486 inhibitors were injected through trachea. ALI patients’ peripheral blood and LPS-induced acute lung injury in mice had significantly higher miR-486-5p levels than control subjects. Inhibition of miR-486-5p by injection with antagomiR-486-5p markedly reduced LPS-induced lung inflammation. Moreover, knockdown of miR-486-5p can reduce protects A549 cell against LPS-induced injury and its corresponding inflammatory response. In addition, Mechanistic analysis indicated that miR-486-5p on the occurrence of ALI is related to the inhibition of OTUD7B activity, which induces the downregulation of inflammatory in ALI. Our results identified miR-486-5p independently associated with ALI. miR-486-5p can mediate the formation of ALI by promoting inflammation.
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Metadaten
Titel
MicroRNA-486-5p Promotes Acute Lung Injury via Inducing Inflammation and Apoptosis by Targeting OTUD7B
verfasst von
Qiang Luo
Jun Zhu
Qian Zhang
Jie Xie
Chengla Yi
Tianyu Li
Publikationsdatum
01.06.2020
Verlag
Springer US
Erschienen in
Inflammation / Ausgabe 3/2020
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-020-01183-3

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