Background
Methods
Participants
Isolation of T-cells and preparation of RNA
Labeling and hybridization to microRNA 2.0 microarrays
Labeling and hybridization to U133Plus 2.0 DNA microarrays
Data analysis of miR_2.0 microarrays
Data analysis of DNA microarrays U133Plus 2.0
Computational methods to identify microRNA functions and mRNA targets
Small nucleolar RNA | Fold change MS/Control | P-value |
---|---|---|
mgU12-22-U4-8_s_st | 0.66 | 0.037 |
mgU2-25-61_s_st | 0.84 | 0.041 |
SNORD119_st | 0.77 | 0.03 |
U104_st | 0.77 | 0.027 |
U105_st | 0.83 | 0.046 |
U105B_st | 0.39 | 0.023 |
U17b_st | 0.53 | 0.025 |
U17b_x_st | 0.58 | 0.02 |
U27_st | 0.57 | 0.004 |
U27_x_st | 0.55 | 0.01 |
U31_st | 0.71 | 0.031 |
U31_x_st | 0.69 | 0.035 |
U42B_x_st | 0.79 | 0.042 |
U56_st | 0.43 | 0.031 |
U56_x_st | 0.33 | 0.005 |
U58A_st | 0.85 | 0.02 |
U59A_st | 0.76 | 0.014 |
U61_st | 0.66 | 0.01 |
U79_st | 0.81 | 0.028 |
U80_st | 0.70 | 0.018 |
U83_st | 0.65 | 0.02 |
U83A_x_st | 0.80 | 0.03 |
U84_x_st | 0.75 | 0.029 |
v11_hsa-miR-768-3p_st | 0.74 | 0.028 |
v11_hsa-miR-768-5p_st | 0.86 | 0.025 |
ACA16_st | 0.76 | 0.014 |
ACA16_x_st | 0.69 | 0.002 |
ACA24_x_st | 0.68 | 0.008 |
ACA44_s_st | 0.50 | 0.048 |
ACA48_x_st | 0.68 | 0.005 |
ACA54_st | 0.45 | 0.003 |
ACA7_s_st | 0.40 | 0.002 |
ACA7B_s_st | 0.52 | 0.01 |
E3_x_st | 0.73 | 0.016 |
ENSG00000201009_s_st | 0.58 | 0.033 |
ENSG00000206903_s_st | 0.54 | 0.031 |
ENSG00000206913_s_st | 0.40 | 0.002 |
ENSG00000212615_x_st | 0.77 | 0.014 |
ENSG00000239031_st | 1.15 | 0.039 |
ENSG00000252049_st | 1.10 | 0.046 |
HBII-210_st | 0.42 | 0.028 |
HBII-436_st | 0.81 | 0.018 |
HBII-55_st | 0.62 | 0.04 |
HBII-85-17_x_st | 0.85 | 0.031 |
HBII-85-29_st | 0.65 | 0.004 |
HBII-85-3_x_st | 0.81 | 0.003 |
HBII-85-9_x_st | 0.87 | 0.043 |
HBII-99_st | 0.51 | 0.011 |
hp_hsa-let-7d_st | 0.90 | 0.044 |
hp_hsa-mir-423_s_st | 0.52 | 0.023 |
Real-time PCR analysis of microRNA expression
Real-time PCR analysis of mRNA expression of TNFSF14
Immunoassay analysis of TNFSF14
Statistics
Results
Differentially expressed microRNA in T-cells between MS patients and controls
microRNA | microRNA microarray | Impact of microRNA on its targets (mRNA microarray) | ||
---|---|---|---|---|
Fold change MS/Control |
P-value
| TargetScan | Miranda | |
hsa-miR-494 | 0.14 | 3.1 × 10-05 | 1.2 × 10-24 | 6.0 × 10-81 |
hsa-miR-15b | 0.14 | 2.5 × 10-03 | 4.1 × 10-34 | 5.5 × 10-15 |
hsa-miR-30c | 0.15 | 7.7 × 10-04 | 2.8 × 10-13 | 5.0 × 10-58 |
hsa-miR-23a | 0.16 | 7.8 × 10-03 | 2.2 × 10-23 | 9.8 × 10-63 |
hsa-miR-197 | 0.19 | 5.2 × 10-06 | 1.6 × 10-12 | 1.2 × 10-06 |
hsa-miR-1260b | 0.21 | 9.4 × 10-07 | NA | 2.3 × 10-16 |
hsa-miR-125a-5p | 0.22 | 8.2 × 10-03 | 1.2 × 10-43 | 5.3 × 10-13 |
hsa-miR-361-5p | 0.24 | 1.4 × 10-02 | 1.8 × 10-15 | 3.9 × 10-32 |
hsa-miR-320d | 0.26 | 2.8 × 10-03 | 2.0 × 10-09 | 7.4 × 10-36 |
hsa-miR-423-3p | 0.26 | 1.6 × 10-02 | 5.0 × 10-13 | 1.8 × 10-03 |
hsa-miR-1280 | 0.29 | 4.7 × 10-03 | 8.2 × 10-56 | 8.6 × 10-04 |
hsa-miR-663 | 0.32 | 3.5 × 10-03 | 5.9 × 10-70 | 1.8 × 10-28 |
hsa-miR-423-5p | 0.33 | 2.6 × 10-02 | 6.1 × 10-96 | 5.7 × 10-36 |
hsa-miR-99b | 0.36 | 2.4 × 10-02 | 3.4 × 10-04 | 7.6 × 10-03 |
hsa-miR-339-5p | 0.37 | 4.9 × 10-02 | 1.5 × 10-35 | 2.5 × 10-07 |
hsa-let-7a | 0.37 | 2.9 × 10-02 | 1.9 × 10-04 | 3.2 × 10-09 |
hsa-miR-1979 | 0.38 | 1.2 × 10-02 | NA | 8.1 × 10-09 |
hsa-miR-3178 | 0.39 | 1.0 × 10-02 | NA | NS |
hsa-miR-625 | 0.41 | 3.2 × 10-02 | 3.6 × 10-62 | 2.3 × 10-09 |
hsa-miR-150 | 0.65 | 3.7 × 10-02 | 2.1 × 10-68 | 2.1 × 10-11 |
hsa-miR-3153 | 1.14 | 4.5 × 10-02 | NA | 2.4 × 10-09 |