Background
Ovarian cancer is a malignancy with with the fifth mortality in female malignant tumors and the highest mortality rate in gynecological cancers, of which epithelial ovarian carcinoma (EOC) is the most common pathologic type accounting for 85–90%. It is estimated that there will be 22,280 Americans diagnosed with ovarian cancer in 2016, and 14,240 of them will die from the disease [
1]. The high mortality rate of ovarian cancer is associated with the difficulties of early detection, because most patients are not diagnosed until late stage (stage III or IV) in their disease [
2]. Besides, for the patients of ovarian cancer, the majority experience relapse within 2 years [
3]. Chemotherapy plays an important role in the therapy for ovarian cancer, but chemoresistance during chemotherapy makes treatment particularly challenging. The chemoresistance has been one of the main reasons for the high mortality of ovarian cancer [
4]. Therefore, it is urgent to discover new treatment strategies for reducing the occurrence of chemoresistance to help improve prognosis.
As a class of small non-coding RNA molecules, miRNAs are endogenously expressed, single-stranded and 19–25 nucleotides long [
2,
5,
6]. MiRNAs, as transcriptional repressors, regulate gene expression by directly binding the 3’ untranslated region of their target miRNAs [
2,
5,
6]. Numerous studies had proved that miRNAs are involved in regulation of almost all cellular processes including proliferation and apoptosis [
2,
5‐
7]. Recently, miRNAs have been reported to either promote carcinogenesis by inhibiting tumor suppressors or suppress tumor development by acting as down-regulate oncogenes in ovarian cancer: downregulated miRNAs (including let-7a/b/d/f, miR-31, 34abc, 92a, 99b, 125b, 127, 152, 155 and 199a), and over-expressed oncogenic miRNAs (such as miR-18a, 20a, 21, 23a/b, 29a, 92, 93, 126, 141, 199a-3p, 200b/c and 429) [
2,
8‐
12]. Moreover, about 27 dysregulated miRNAs have been linked to chemo-resistance to taxanes or platinum compounds in ovarian cancer [
13]. Over-expression of miR-27a and miR-514 or loss of let-7i/let-7e have been related to resistance to taxanes and/or platinum [
13,
14]. MiR-93 and MiR-214 can promote cisplatin resistance by targeting PTEN/AKT [
9,
15]. MiR-376c can promote cisplatin resistance by targeting ALK7 [
9]. MiR-214 can promote paclitaxel resistance by targeting BCL10 and caspase-7, and miR-433 or miR-182 can promote paclitaxel resistance by targeting MAD2 or PDCD4 [
9]. MiR-141 can promote platinum resistance by targeting KEAP1 [
9]. Two studies have reported that miRNA is involved in the development of chemoresistance in ovarian cancer by inhibiting pro-apoptotic signal pathway [
4,
8]. Additionally, up-regulation of miR-300 can inhibit cellular apoptosis through TGF-β, resulting in chemoresistance enhancement in ovarian cancer cells [
16]. Particularly, it has been reported that miRNA-1307 is over-expressed in chemoresistant ovarian cancer tissues compared to the chemosensitive counterparts, indicating that miR-1307 is associated with the chemoresistance in ovarian cancer [
7]. However, up to now, the functional study of miR-1307 has been limited, and the chemoresistance mechanism of miR-1307 in ovarian cancer is still unclear.
In the present study, we evaluated the miR-1307 expression in chemoresistant ovarian cancer cell line A2780/Taxol and the function of miR-1307 for chemoresistance in various kinds of ovarian cancer cells. We also performed a systematic analysis on miR-1307 for its role in ovarian cancer chemoresistance and a preliminary analysis on the mechanism. Finally, our results indicated miR-1307 could promote ovarian cancer chemoresistance by reducing the ING5 expression in vitro and in vivo. Thus, miR-1307 might serve as a therapeutic target for ovarian cancer.
Methods
Cell culture
Human ovarian cancer cell line A2780, SKOV3 and paclitaxel-resistant A2780 (A2780/Taxol) were obtained from the the Committee of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The SKOV3 and A2780/Taxol cells were maintained in RPMI-1640 medium, while the A2780 cellswere maintained in Dulbecco’s modified Eagle’s medium (DMEM). Ten percent fetal bovine serum (FBS), 100 units · mL−1 penicillin and 100 μg · mL−1 streptomycin were added in the above. All cells were incubated at 37 °C in a humidified atmosphere of 95% air and 5% CO2.
Chemoresistance profling (IC50 determination) [17, 18]
Cells were seeded in triplicate in 96-well plates at the density of 5 × 103/well and treated differently or not in the various kinds of ovarian cancer cells for 72 h. Cell survival was then measured by a thiazolyl blue tetrazolium bromide (MTT, 490 nm reading)-based cell proliferation assay. Both the linear regression parameters and the IC50 (the concentration of drug required for 50% of cells to be killed) with the no-drug control as the reference were calculated. The relative chemoresistance was presented as the fold for each of the cell line over the lowest IC50.
RNA analysis
Total RNA was isolated from the cells at the logarithmic phase using TRIzol reagent (Tiangen Biotech Co., Ltd., Beijing, China). For mRNA analysis, cDNA primed by oligo-dT was generated using a PrimeScript RT reagent kit (Tiangen Biotech Co., Ltd., Beijing, China), and the mRNA levels of the genes were quantifed by duplex-QRT-PCR analysis using TaqMan probes with a different fuorescence for the β-actin (provided by Shing Gene, Shanghai, China) and a FTC-3000P PCR instrument (Funglyn Biotech Inc, Canada). PCR cycle conditions are: 95 °C for 5 min, followed by 40 cycles of 95 °C 10s, 60 °C 20s, 72 °C 20s, and 78 °C 20s. Using the 2-∆∆Ct method, gene expression was normalized to β-actin and then compared between groups. The sequences of the primers and probes used for the QRT-PCR analysis were as follows:
-
miR-1307 F: 5’-AACTCGGCGTGGC -3’,
-
miR-1307 R: 5’-GAGCAGGCTGGAGAA-3’.
-
U6 was used as internal control in RT-PCR:
-
U6 F: 5’-GCTTCGGCAGCACATATACTAAAAT-3’,
-
U6 R: 5’-CGCTTCACGAATTTGCGTGTCAT-3’.
-
ING5 F: 5’- TCCAGAACGCCTACAGCAAG -3’,
-
ING5 R: 5’- TGCCCTCCATCTTGTCCTTC -3’,
-
ING5 probe: 5’- CY5-CGACAAAGTGCAGCTG GCCATGC -3’.
Experiment was performed independently for three times.
Reagents for the transient transfection assays
The miR-1307 mimics, miR-1307 ASO, siRNA and the scramble sequence control were supplied by Guangzhou Ribobio (Guangzhou, China). The transfection was used by Lipofectamine-2000 in accordance with the manufacturer’s guidelines (Invitrogen). The final concentration of antisense oligodeoxynucleotide (ASO) con and miR-1307 ASO was 100 nM. Untreated cells were designated as the blank control group.
The siRNA sequences used for ING5 interference in this study was as follows:
Cell proliferation
Cell proliferation ability was compared by colony formation assays. 6-well plates were used to seed cell suspensions, 300 cells per well. After incubated, cells were fixed in methyl hydrate for 10 min. Then the colonies were stained and they were counted by using an optical microscope.
Western blot
A quantity of 30 ug of lysates per sample was separated by SDS–PAGE using 10% polyacrylamide gels and transferred to PVDF membrane which was subsequently incubated with the antibody (see the below) for 4 °C overnight, and corresponding were immunodetected by incubation with HRP (horseradish peroxidase)-linked related secondary antibody using an ECL detection kit (Pierce Biotechnology). The antibodies used in this article were below: rabbit monoclonal Ki67 antibody (sc-15,402, 1:1000 dilution), mouse monoclonal PCNA antibody (sc-25,280, 1:1000 dilution), mouse monoclonal caspase-3 antibody (sc-65,496, 1:1000 dilution), mouse monoclonal caspase-7 antibody (sc-81,655, 1:1000 dilution), rabbit monoclonal ING5 antibody (10665-1-AP, 1:1000 dilution), mouse monoclonal β-actin antibody (sc-8432, 1:2500 dilution), the secondary antibodies preparation was either anti-rabbit (1:5000) or anti-mouse (1:5000).
Apoptosis analysis by FITC immunofluorescence
Cells were harvested and rinsed with phosphate-buffered saline (PBS) twice. Then, 5 μl of fuorescein isothiocyanate (FITC)-labeled enhanced Annexin V and 5 μl (20 μg/ml) of propidium iodide were added to the 100 μl cell suspension. Following incubation in the dark for 15 min at room temperature, the samples were diluted with 400 μl PBS. Then dropped the cell suspension on the slide after dyeing, and covered it with a cover glass cell for detection by using fluorescence microscope. Annexin V-FITC showed the green fluorescence, and DAPI showed the blue fluorescence. The results were analyzed according to the manufacturer’s instructions. The experiments were performed independently three times, and a representative result was shown herein.
Analysis of miRNA target genes and gene ontology analysis
Target Scan, miRanda and Diana microT-CDS were used to analyze the potential target genes for miR-1307. GOstat was used for gene enrichment analysis. The DAVID database was used for signal transduction enrichment Analysis.
Dual-luciferase reporter assay [19]
5 × 104 cells per well in 12-well plates were cultured without antibiotics overnight and then transfected with cloned ING5 wild-type 3’-UTR target sequence and mutant 3’-UTR by using Lipofectamine 2000 (Invitrogen). After 48 h, cells were washed with PBS, subjected to lysis, and their luciferase activities measured by using a dual luciferase assay kit (Promega). The results were normalized against Renella Luciferase. Each reporter plasmid was transfected at least three times. The wild sequence for ING5: 3’ UTR: AGCUGGCCCUCGACGCCCGGACC, while mutant sequence was 3’UTR: ACCAGCCGCUCGACGCCCGGACC. Both of them were designed and purchased from Shanghai Genechem Co., Ltd (Shanghai, China).
Xenografts assays in vivo
The animal study was carried out in accordance with the guidelines approved by the Animal Experimentation Ethics Committee of affiliated hospital of Zunyi medical college. The protocol was approved by the committee, all surgery was performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. Athymic Balb/c nude mice (aged 5 weeks) were provided by Slac Laboratory Animal Co. Ltd. (Shanghai, China), and the mice were housed in a pathogen-free animal facility and randomly assigned to the control or experimental group (six mice per group) [
20]. 2 × 10
6 A2780 cells were suspended in 0.1 mL of serum-free RPMI 1640 was injected into the right subaxillary of each mouse [
20]. And the mice were divided into 5 groups which were treated differently on the day 14 (the tumor volume reached about 50–100 mm
3): The mice were injected with MOCK (transfected with vector), Taxol, ASO con, miR-1307 ASO, or miR-1307 ASO + Taxol once every 2 days (8 times in all) [
17]. The final concentration of MOCK, ASO con and miR-1307 ASO was 100 nM intratumorally injected into A2780 cells every time, respectively (0.01 mol). And taxol was injected 10 mg/kg for the mice intraperitoneally. The tumors were measured by vernier caliper on the day 14, 17, 21, 23, 26, and 29. The following formula was used for calculation of tumor volume: Tumor volume (mm
3) = tumor length (mm) × tumor width (mm) × tumor width (mm)/2. 29 days after inoculation, mice were killed, and the final volume of tumor tissues was determined.
Statistical analysis
The experiments’ results in vitro and in vivo were depicted as mean ± SD. Student’s two-sided t-test was used to compare values of test and control samples. All calculations were performed with the SPSS 18.0 and the level of significance was set to P < 0.05.
Discussion
MiRNAs play important roles in almost all tumor cellular processes [
2,
5‐
12], which either promote carcinogenesis or suppress tumor development in ovarian cancer [
2,
8‐
12]. Particularly, many miRNAs including miR-1307 have been linked to chemo-resistance to many kinds of chemotherapy drugs in ovarian cancer [
2,
4,
7‐
9,
13‐
16]. Recently, miR-1307 was found to be up-regulated in a variety of cancers, such as breast cancer, liver cancer, colorectal tumor and ovarian cancer [
7,
21‐
24]. For example, miR-1307-3p was found to be useful for detecting breast cancer in the early stage [
21]. And methylated miR-1307 was identified in subjects who went on to develop breast cancer [
22]. MiR-1307 in chronic hepatitis C was identified in a recent report [
23]. Even a report has indicated that rs7911488 C-allelic pre-miR-1307 binds to MBNL1 and infers with Dicer processing, and then leads to the decreased miR-1307 and increased Bcl-2 expression, thus representing an important process in the initiation of colorectal cancer [
24]. Nevertheless, up to now, we have found only one report about the correlation between miR-1307 and the ovarian cancer chemoresistance, which indicated that miR-1307 was associated with the chemoresistance in ovarian cancer [
7]. In our article, to demonstrate the function of miR-1307 in chemoresistance and to explore its chemoresistance mechanism, we firstly showed that miR-1307 was over-expressed in chemoresistant ovarian cancer cell line A2780/Taxol, which was consistent with the literature report [
7]. And we also found that over-expression or loss of miR-1307 promoted or inhabited chemoresistance in ovarian cancer cells. Besides, to explain the chemoresistance caused by miR-1307, a preliminary analysis was needed, and we found that over-expression of miR-1307 promoted proliferation and inhibited apoptosis. Moreover, we confirmed that the inhibitor of growth 5 (ING5) as a functional target of miR-1307 in ovarian cancer cells. Thus, our discovery demonstrated that miR-1307 might be a tumorous promoter in ovarian cancer cells, and abnormal alteration of miR-1307-ING5 interaction might contribute to the chemoresistant in ovarian cancer.
It is well known that most microRNAs regulate target mRNAs by binding to the 3’ UTR of target genes in a post-transcriptional manner [
18,
19]. Therefore, establishing the interrelationship of miRNA and its target genes helps to better understand the molecular mechanism and provide potential therapeutic targets for the clinical treatment of cancers. Although the anti-apoptosis protein Bcl-2, which was a direct target of miR-1307, had been reported to be over-expressed in colorectal cancer [
24], we found that ING5 was a target gene of miR-1307 by means of chip analysis and Target Scan software. Meanwhile, the direct targeting was further supported through luciferase reporter assay. As a result, these data suggested that the regulation mechanism of miR-1307 might vary in different kinds of cancers. To confirm the hypothesis that miR-1307 promoted ovarian cancer cell chemoresistance by targeting the ING5 expression, a preliminary analysis was performed, and the regulatory mechanism of miR-1307 and downstream gene ING5 was explored. Recently, a report suggested that miR-193a-3p-regulated ING5 gene activated the DNA damage response pathway and inhibited multi-chemoresistance in bladder cancer [
18]. Different from the result owing to the different types of cancer, our results indicated ING5 was targeted by miR-1307, yet we could also use the similar method to prove their correlation [
18,
19]. As shown in the present study, we could see the inhibiting effect of miR-1307 ASO by increasing the ING5 expression against chemoresistant ovarian cancer in vitro. Of course, the similar inhibiting effect was demonstrated in vivo. However, it was also needed to demonstrate the effect in other types of animal models before the clinical trial. To further explore the mechanism, we analyzed the protein expressions following different miR-1307 treatment in various kinds of ovarian cancer cells, and the results indicated the downstream gene ING5 of miR-1307 in vitro. Furthermore, the up-regulated ability of cell apoptosis and down-regulated ability of chemoresistance following the loss of miR-1307 was rescued by adding ING5 siRNA in vitro. The methods by analyzing the mechanism were similar with the recent reports [
18,
19], and our results indicated that miR-1307 could promote ovarian cancer chemoresistance by targeting ING5.
ING5, one of the ING family genes and as a transcriptional co-activator, has been demonstrated to play important roles in the development of tumor [
18,
25‐
34]. Some studies reported that the decreased ING5 protein and its cytoplasmic translocation were observed in many tumors, including bladder cancer [
18], lung cancer [
25,
27], oral squamous cell carcinoma [
28,
30], head and neck squamous cell carcinoma [
31], colorectal [
32], pancreatic cancer [
29], gastric carcinoma [
26,
33] and ameloblastoma [
34]. In addition, it was reported that ING5 could affect adhesion, migration, invasion, proliferation and apoptosis of tumor cells [
18,
25‐
34]. Bax and GADD45 were ING5 target apoptotic genes [
35,
36]. The anti-proliferative effect of ING5 depended on its interaction with INCA1 [
37]. It was worth noting that ING5-mediated chemoresistance was closely related to their apoptotic resistance, Akt activation, and the over-expression of chemoresistance-related genes [
18,
26,
30]. For the inhibiting effect on epithelial-mesenchymal transition (EMT), ING5 inhibited lung cancer aggressiveness [
27], and ING5 suppressed PI3K/Akt in breast cancer [
36]. In spite of these the correlation between ING5 and ovarian cancer still remained unclear. Various studies reported that ING5 expression was down-regulated or ING5 was a negative regulator of chemoresistance in many kinds of tumors [
18,
25‐
34]. Consistent with these results, we demonstrated that ING5 inhibited cell proliferation, promoted cell apoptosis and inhabited chemoresistance in ovarian cancer.
Chemotherapy is a double-edged sword, which leads to the treatment and the chemoresistance for tumor. This provides a theoretical basis for the therapy, which needs chemotherapy combined with the inhibition of miR-1307. Even so, future studies should also determine the ING5 feedback effect (the pathways associated with ING5 in the above) on miR-1307 during the chemoresistance in ovarian cancer cells, and the above mechanism should be further investigated in various kinds of ovarian cancer cells.
Acknowledgement
This work was supported by Qiankehe LH [2015] No.7450.