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European Journal of Trauma and Emergency Surgery

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13.10.2022 | Original Article

miR-181c, a potential mediator for acute kidney injury in a burn rat model with following sepsis

verfasst von: Yonghui Yu, Xiao Li, Shaofang Han, Jingjie Zhang, Jing Wang, Jiake Chai

Erschienen in: European Journal of Trauma and Emergency Surgery | Ausgabe 2/2023

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Abstract

Background

The miRNA profile is changed after burn or sepsis and is involved in regulating inflammatory reactions. However, the function and molecular mechanism of miRNAs in regulating burn sepsis-induced acute kidney injury (AKI) are still unclear.

Methods

In this study, animal and cell sepsis models were established after burned rats were injected with lipopolysaccharide (LPS) or NRK-52E cells treated with LPS, respectively. Cytokine expression, inflammatory cell infiltration, serum creatinine (Scr) and kidney injury molecule-1 (KIM-1) levels were analysed after the indicated treatments.

Results

Burn sepsis increased the expression of inflammatory factors (TNF-α and IL-1β) and chemokines (MIP-1α, MIP-2 and MCP-1). Moreover, burn sepsis promoted macrophage and neutrophil infiltration into the kidney and upregulated the levels of Scr and KIM-1 in the kidney and urine. Ectopic expression of miR-181c significantly reduced LPS-induced TLR4 protein expression, suppressed KIM-1 mRNA levels and subsequently inhibited the activation of inflammatory genes (TNF-α and IL-1β) and chemokine genes (MIP-1α, MIP-2 and MCP-1).

Conclusions

Our results demonstrated that miR-181c could suppress TLR4 expression, reduce inflammatory factor and chemokine secretion, mitigate inflammatory cell infiltration into the kidney and downregulate KIM-1 expression, which might ultimately attenuate burn sepsis-induced AKI.

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Titel: miR-181c, a potential mediator for acute kidney injury in a burn rat model with following sepsis
verfasst von: Yonghui Yu
Xiao Li
Shaofang Han
Jingjie Zhang
Jing Wang
Jiake Chai
Publikationsdatum: 13.10.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Trauma and Emergency Surgery / Ausgabe 2/2023
Print ISSN: 1863-9933
Elektronische ISSN: 1863-9941
DOI: https://doi.org/10.1007/s00068-022-02124-5

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miR-181c, a potential mediator for acute kidney injury in a burn rat model with following sepsis