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01.12.2019 | Review | Ausgabe 1/2019 Open Access

Journal of Hematology & Oncology 1/2019

Mismatch repair deficiency/microsatellite instability-high as a predictor for anti-PD-1/PD-L1 immunotherapy efficacy

Zeitschrift:
Journal of Hematology & Oncology > Ausgabe 1/2019
Autoren:
Pengfei Zhao, Li Li, Xiaoyue Jiang, Qin Li
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s13045-019-0738-1) contains supplementary material, which is available to authorized users.
Pengfei Zhao and Li Li contributed equally to this work.

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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
In recent years, immunotherapy has become the focus of the revamped cancer therapeutic paradigm. Immunotherapy has brought amazing and long-lasting tumor remission for several common solid malignancies and refractory malignancies [16]. Nonetheless, the extensive clinical application of immunotherapies has been limited because some tumors show relatively poor efficacy and low response rates [1, 2]. Therefore, biomarkers are urgently needed to distinguish the sensitive patients and to predict therapeutic response. Ample evidence supports programmed death-ligand 1 (PD-L1) or programmed cell death-1 (PD-1) expression, tumor mutational burden (TMB), numbers of tumor-infiltrating lymphocytes (TILs), peripheral blood lymphocyte count, mismatch repair deficiency (dMMR), and microsatellite instability-high (MSI-H) as predictive biomarkers that guide the clinical application of immune checkpoint blockade (ICB) therapies [7]. Among many indicators, dMMR and MSI-H show unique advantages. Tumors with dMMR or MSI-H are sensitive to ICB, particularly to PD-1 and PD-L1 inhibitors. It is worth emphasizing that dMMR or MSI-H could identify responders regardless of tumor location and tumor type, that is, they have the ability to guide different tumor immunotherapies in the same manner. Subsequently, the US Food and Drug Administration (FDA) approved the indication of ICB for all dMMR/MSI-H solid tumors [8]. In this review, we elaborate on the expression of MMR/MSI in multiple tumors, the predictive value of MMR/MSI-H in ICB treatment, the relationship between MSI-H and other predictor markers.

Mismatch repair proficiency/deficiency and microsatellite instability

The DNA mismatch repair (MMR) system, which exists extensively in organisms from prokaryotes to eukaryotes, is a highly conserved repair mechanism in cellular evolution. MMR was first found as a causative germline alteration in patients with Lynch syndrome in 1993 and was termed a microsatellite [912]. The MMR system plays key roles in identifying and repairing mismatched nucleotides during genetic recombination or as a result of damage caused by external physical or chemical insults. MMR guarantees genomic integrity and stability and avoids insertions and deletions of abnormal DNA at microsatellites. The MMR system comprises a series of specific DNA mismatch repair enzymes and is usually dependent on four key genes: mutL homologue 1 (MLH1), postmeiotic segregation increased 2 (PMS2), mutS homologue 2 (MSH2), and mutS 6 (MSH6). MLH1, PMS2, MSH2, and MSH6 proteins are mainly detected through immunohistochemical methods in the clinic. MSH2/MSH6 heterodimers are responsible for binding to the initial DNA mismatched base errors (including single-base mismatch and incorrect insertion or deletion loop mismatch) by conformational changes, and MLH1/PMS2 heterodimers are in charge of the excision and synthesis of corrected DNA chains in the mismatch site (see Fig. 1a). If one or more proteins are not expressed or are dysfunctional, the status is called dMMR; otherwise, the status is considered mismatch repair proficient (pMMR). MLH1 and MSH2 play pivotal roles in the process of MMR by dimerizing and interacting with MSH6 and PMS2. The dysfunction of MLH1 or MSH2 leads to the inactivation of MLH1/PMS2 or MSH2/MSH6 and the degradation of PMS2 or MSH6 (see Fig. 1b). Lynch syndrome is a common hereditary disease that is characterized by germline mutations in MMR genes [13]. Lynch syndrome is associated with multiple cancers, especially colon cancer and endometrial cancer. A lack of MSH2, substantial mutations in the MLH1 or MSH2 genes, MLH1-methylation inactivation, and transcriptional silencing lead to Lynch syndrome [14, 15]. Deletion mutations in MLH1 and MSH2 account for 42–50% and 33–39%; however, MSH6 and PMS2 mutations account for only 7–18% and less than 7%, respectively [1619]. A hypothesis that heterozygous germline deletions in the epithelial cell adhesion molecule (EPCAM) gene as one factor that leads to MSH2 defects has been confirmed, and the addition of EPCAM to the diagnostic panel for Lynch syndrome in MSH2-defective tumors has been advised [19, 20].
The inactivation of MMR genes and MMR protein dysfunction may be the results of germline mutations or spontaneous hypermutation alterations, which may induce microsatellite instability (MSI). More than 100,000 areas of short tandem repetitive DNA sequences are the diagnostic sites of MSI. Two mononucleotide repeats (BAT25 and BAT26) and three dinucleotide repeats (D5S346, D2S123, and D17S250) are the standard sites in panels for MSI testing, as recommended by the National Cancer Institute in 1998 [2123]. If two or more repeats are altered, the tumor is defined as MSI-H; if only one mutated sequence is found, the tumor is considered to be microsatellite instability-low (MSI-L). Otherwise, it is said to have microsatellite stability (MSS). There is a high level of consistency (almost 90–95%) between dMMR and MSI-H among many tumors; therefore, these two terms are used almost interchangeably [24]. MSI-H or dMMR has been widely detected and reported in Lynch syndrome-associated tumors, usually in colorectal cancer (CRC) [12], gastrointestinal adenocarcinoma [23], and endometrial cancer [25]. However, MSI-H or dMMR rarely appears in breast cancer [26], prostate cancer [27], and lung adenocarcinoma [9]. MSI-H varies from 0–31.37% in 39 cancer types. Endometrial carcinoma of the uterine corpus, colon adenocarcinoma, and stomach adenocarcinoma rank in the top 3 in terms of the prevalence of MSI-H, followed by rectal adenocarcinoma, adrenocortical carcinoma, and uterine carcinosarcoma. MSI-H has not been detected in more than ten tumors, as shown in Table 1 [2830]. The prevalence of MSI-H in these studies was mostly derived from tissues of early-stage tumors [2830]. Le DT et al. reported that dMMR occurred more often in early-stage tumors than in late-stage tumors (stage IV) [30]. Venderbosch [31] also found that the incidence of dMMR in metastatic CRC was 5%, which was lower than that (19.72%) in early-stage CRC. MOSAIC, MANTIS, and next-generation sequencing were used to analyze MSI status [2729], as sensitive standardized detection of MSI is necessary.
Table 1
Prevalence of MSI-H in 39 cancer types
Cancer type
MSI-H (%)
Cancer type
MSI-H (%)
Cancer type
MSI-H (%)
Cancer type
MSI-H (%)
UCEC
17.00–31.37
BRCA
0.00–1.53
PRAD
0.60–3.00
KICH
0.00
COAD
6.00–19.72
KIRC
1.47
LUAD
0.53–1.00
KIRP
0.00
STAD
9.00–19.09
OV
1.37–2.00
BLCA
0.49
LAML
0.00
READ
5.73
CHOL
1.35–3.00
NBL
0.45
NPC
0.00
ACC
4.35
THYM
0.81
LGG
0.39
PAAD
0–2.00
UCS
3.00–3.51
LIHC
0.80–3.00
CLL
0.30
PCPG
0.00
CESC
2.62–4.00
HNSC
0.78
GBM
0.25
TGCT
0.00
WT
2.44
SARC
0.78
AML
0.00
THCA
0.00–3.00
MESO
2.41
SKCM
0.00–0.64
CTCL
0.00
UVM
0.00–2.00
ESCA
1.63
LUSC
0.60
DLBC
0.00
  
Abbreviations: UCEC uterine corpus endometrial carcinoma, COAD colon adenocarcinoma, STAD stomach adenocarcinoma, READ rectal adenocarcinoma, ACC adrenocortical carcinoma, UCS uterine carcinosarcoma, CESC cervical squamous cell carcinoma and endocervical adenocarcinoma, WT Wilms tumor, MESO mesothelioma, ESCA esophageal carcinoma, BRCA breast carcinoma, KIRC kidney renal clear cell carcinoma, OV ovarian serous cystadenocarcinoma, CHOL cholangiocarcinoma, THYM thymoma, LIHC liver hepatocellular carcinoma, HNSC head and neck squamous cell carcinoma, SARC sarcoma, SKCM skin cutaneous melanoma, LUSC lung squamous cell carcinoma, PRAD prostate adenocarcinoma, LUAD lung adenocarcinoma, BLCA bladder carcinoma, NBL pediatric neuroblastoma, LGG lower-grade glioma, CLL chronic lymphocytic leukemia, GBM glioblastoma multiforme, AML pediatric acute myeloid leukemia, CTCL cutaneous T cell lymphoma, DLBC diffuse large B cell lymphoma, KICH kidney chromophobe, KIRP kidney renal papillary cell carcinoma, LAML acute myeloid leukemia, NPC nasopharyngeal carcinoma, PAAD pancreatic adenocarcinoma, PCPG pheochromocytoma and paraganglioma, TGCT testicular germ cell tumor, THCA thyroid carcinoma, UVM uveal melanoma

The relationship between MMR and multiple tumors

In this review, MMR gene expression of 12,821 samples from 33 different tumors were pooled and analyzed through The Cancer Genome Atlas (TCGA) database (http://​www.​cbioportal.​org/​). Data from all TCGA cohorts were combined to produce this PanCancer dataset. Values of gene expression from the RNAseq experiment shown in Fig. 2 are log2(x + 1) transformed RSEM values. The expression of MMR genes is different in many tumors and even in the same tumor. Generally, the expression of MSH6 is almost always the highest, while PMS2 expression is the lowest. MLH1 gene expression is observed more often in acute myeloid leukemia, glioblastoma multiforme, and testicular germ cell tumors. MSH6 and MSH2 are expressed more frequently in acute myeloid leukemia, testicular germ cell tumors and uterine carcinosarcoma. However, PMS2 gene expression in the kidney chromophobe and kidney papillary cell carcinoma is higher than in other tumors (see Fig. 2). The correlations among MLH1, PMS2, MSH2, and MSH6 were also demonstrated using the TCGA. Excellent positive correlations were observed among the four MMR genes (all r > 0.97) (see Fig. 3). The good correlations coincide with the heterodimeric characteristic of these four genes.
The survival analysis based on the TCGA suggests a significant association between the expression of MMR genes and prognosis in several tumors. In general, dMMR is correlated with an improved median overall survival (mOS) in most tumors other than head and neck cancer and pancreatic cancer. MLH1− is a prognostic factor in esophageal carcinoma and liver hepatocellular carcinoma (P = 0.049, 0.039, respectively), and MSH2− is correlated with improved mOS of bladder urothelial carcinoma, liver hepatocellular carcinoma, prostate adenocarcinoma, and sarcoma (P = 0.029, 0.002, 0.011, and 0.001, respectively). The mOS of the MSH6− group is prolonged compared with that of the MSH6+ group in patients with bladder urothelial carcinoma (P = 0.015). Except for pancreatic cancer, PMS2− patients exhibit no survival benefit in most cancers (see Additional file 1: Figure S1). A comprehensive analysis indicated that mOS was improved only in esophageal carcinoma and liver hepatocellular carcinoma patients with four MMR gene deficiencies (P = 0.049 and 0.001, respectively) (see Additional file 2: Figure S2).

The predictive value of dMMR/MSI-H in multiple tumors

A strong clinical relationship is observed between MMR status and CRC. dMMR/MSI-H occurs in up to 15% of sporadic colon cancers [32]. Several clinicopathological variables, such as proximal tumor location, advanced age (> 65 years), poor differentiation, diploid DNA content, and the BRAF V600E mutation were found to be associated with the prevalence of MSI-H [32]. MLH1 dysfunction is responsible for most tumors in sporadic CRC patients with dMMR because its transcriptional promoter gene is silenced due to CpG island hypermethylation. The prognostic and predictive values of MMR in CRC are different in early-stage and late-stage. Most results indicated that dMMR is a positive prognostic factor in early-stage (II/III) rather than late-stage (IV) [30, 31, 3336]. Ribic et al. [33] observed that patients with MSI-H had significant increased a 5-year survival rate compared with the MSI-L or MSS counterparts with stage II or stage III CRC who did not receive fluorouracil-based adjuvant chemotherapy (88.0% versus 66.4%, P = 0.004), but the 5-year survival rate of the former group was not significantly increased in patients who received adjuvant chemotherapy (70.7% versus 75.5%, P = 0.66). Guastadisegni [34] demonstrated that the odds ratio (OR) for OS associated with MSI-H was 0.6 (P < 0.0001) in patients with stages I–IV and 0.65 (P < 0.0001) in patients with stages II–III CRC and that the OR for disease-free survival (DFS) was 0.58 (P < 0.0001). They pooled the data and showed that MSS patients treated with 5-fluorouracil had a better prognosis but that the benefit was not obvious for MSI-H CRC patients (OR 0.52, 95% CI 0.4–0.6, P < 0.0001 versus OR 0.69, 95% CI 0.3–1.5, P = 0.10). Sargent et al. [35] also concluded that patients with stages II–III CRC with pMMR exhibited improved DFS (hazard ratio [HR] 0.67, 95% CI 0.48–0.93, P = 0.02) resulting from adjuvant therapy compared with those who underwent surgery alone. Regarding the predictive value of MMR status to the response to adjuvant irinotecan- or oxaliplatin-based treatment in stage III CRC patients is contradictory [3740]. MSI-H or dMMR has a good prognostic predictive value in early-stage CRC settings without adjuvant chemotherapy and plays a negative predictive role for adjuvant fluorouracil-based chemotherapy in patients with resected CRC. However, the value of dMMR/MSI-H in metastatic CRC (mCRC) is opposite to that of early-stage CRC. A meta-analysis confirmed that mCRC patients with dMMR had poorer survival compared with pMMR patients, which might be due to a BRAF V600E mutation. The median progressive-free survival (mPFS) was 6.2 months in dMMR patients and 7.6 months in pMMR patients (HR 1.33, P = 0.001), while the mOS was 13.6 months and 16.8 months, respectively (HR 1.35, P = 0.001) [31]. The study of Mayo Clinic showed that mCRC patients with MSI-H had earlier disease recurrence (12.9 months vs. 20.9 months, P = 0.034) and poorer OS (28.1 months vs. 37.4 months, P = 0.99) than MSS patients [36]. Detection of a BRAF V600E mutation is recommended in sporadic MSI tumors with silenced MLH1, as the presence of a BRAF V600E mutation strongly suggests that the etiology of the disease is sporadic [41].
The predictive value of dMMR was also investigated in other tumors. dMMR has been demonstrated in 20–40% of endometrial cancers [25, 42], but data on its prognostic value are controversial [25, 43, 44]. A meta-analysis including 23 studies found no association between OS (HR 2.0, P = 0.11) or DFS (HR 1.31, P = 0.66) and dMMR [45] in endometrial cancer. MSI frequency or dMMR expression in ovarian tumors varied from 5–13% [4648] in MSI patients and from 2–29% in dMMR patients [49]. dMMR as a positive predictive biomarker for survival or response in ovarian cancers has not been confirmed [50, 51]. In gastric cancer (GC), MSI-H has been identified in approximately 10–20% of tumors, and patients with MSI-H demonstrate an improved survival compared with patients with MSS [52, 53]. However, data on the predictive value of MSI for GC patients treated with fluorouracil-based chemotherapy are conflicting [54, 55]. In a study on melanoma, the expression of MSI was increased when the disease progressed from benign to malignant and then to metastatic [56]. Related studies in other tumors are few in number and limited, and therefore, evidence of the prognostic value is insufficient.

dMMR/MSI-H predicts the efficacy of anti-PD-1/PD-L1 immunotherapy

Anti-PD-1/PD-L1 immunotherapies have led to tremendous success in treating certain cancers, such as melanoma, non-small cell lung cancer (NSCLC), mCRC, renal cell carcinoma, bladder cancer, head and neck squamous cell carcinoma, classical Hodgkin’s lymphoma, and Merkel cell carcinoma [16, 5763]. However, only a small fraction of patients with these malignancies are sensitive to ICB therapies. For patients with NSCLC or metastatic melanoma [1, 2, 60], only 15.2–20% of patients who received single ICB therapy achieved objective response and 33.2–49% of patients obtained disease control. The precise recognition of targeted patients has always been the common goal of the researchers. Recent works suggest that MMR status could serve as a candidate biomarker and predict the responses of patients with solid tumors to ICB, irrespective of cancer type. Impressive results of pembrolizumab in patients with dMMR or MSI-H tumors after progression from prior chemotherapies have been shown in the KEYNOTE-016, 164, 012, 028, and 158 trials [8]. 149 patients with 15 different MSI-H or dMMR tumors were enrolled in the five trials. Patients with MSI-H/dMMR non-CRC were prospectively enrolled in single-arm trials (KEYNOTE-016, 158) or retrospectively identified in multi-cohort trials (KEYNOTE-012, 028) or in patients with one of 10 rare tumor types (KEYNOTE-158). 28 patients with CRC and 30 patients with non-CRC were included in KEYNOTE-016 trial, and 61 patients with CRC were enrolled in the initial interim analysis of KEYNOTE-164 trials. There were 6, 5, and 19 patients enrolled in KEYNOTE-012, 028, and 158 trials, respectively. Patients received pembrolizumab at 200 mg intravenously every 3 weeks in KEYNOTE-164, 158, and at 10 mg/kg intravenously every 2 weeks in KEYNOTE-028, 016, and 012 for 2 years until rapid disease progression or severe toxicity. The summarized results showed the overall response rate (ORR) was 39.6% (95% CI 31.7–47.9); moreover, the duration of response ranged from 1.6 to 27 months, with 78% of responses sustaining longer than 6 months [8, 64]. Pembrolizumab led to a durable response and survival benefits in patients with dMMR chemotherapy-refractory progressive metastatic tumors. Based on the above results, in 2017, the FDA accelerated the approval of pembrolizumab as a second- or higher-line choice for the treatment of patients with unresectable or metastatic dMMR/MSI-H solid tumors, irrespective of tumor type or site. This approval for a drug indication is noteworthy because it is the first time that one biomarker defined an indication regardless of the primary tumor site. However, further clinical trials consisting of sufficient number of patients and adequate follow-up are necessary to verify the efficacy of pembrolizumab in patients with dMMR/MSI-H.
As the partial published results of the KEYNOTE-016 trial, 41 patients were enrolled and assigned to the CRC with dMMR (11 patients), CRC with pMMR (21 patients), and non-CRC with dMMR (9 patients) groups. The outcome showed that the immune-related ORR and PFS rates were 40% and 78% in dMMR CRC patients, 71% and 67% in dMMR non-CRC patients, and 0% and 11% in pMMR cancers. Both the mPFS and mOS were not reached in dMMR CRC patients in contrast to the 2.2 and 5.0 months observed in pMMR CRC patients [65]. The KEYNOTE-164 and 158 trials both reported the positive evidence to support the FDA approval of pembrolizumab. The relative update data are showed in the following description. Sixty-three patients with unresectable locally advanced or metastatic dMMR/MSI-H CRC after the failure of prior fluoropyrimidine-, oxaliplatin-, and irinotecan-based chemotherapy were enrolled in the KEYNOTE-164 trial, and 77 patients with any advanced MSI-H solid tumors after one or more prior regimen, except CRC, were enrolled in the KEYNOTE-158 trial. Both trials indicated similar objective response rates (ORRs) (32% and 37.7%), mPFS (4.1 months and 4.3 months), and 6-month OS rates (87% and 73%) [66, 67]. In the KEYNOTE-164 trial, the 12-month PFS rate was 41% and the 12-month OS rate was 76%.
The efficacy of nivolumab was also investigated in patients with dMMR/MSI-H tumors. The open-label, multicenter, phase II study CheckMate 142 evaluated the efficacy of nivolumab in 74 mCRC patients with dMMR/MSI-H tumors that progressed during or after one-line conventional chemotherapy. Patients received 3 mg/kg nivolumab intravenously every 2 weeks until progressive disease, unacceptable toxicity, or others. In all, 31.1% of patients achieved an objective response, 69% of patients had disease control for more than 12 weeks, and the PFS and OS rates at 12 months were 50% and 73%, respectively. The medium duration of responses was not reached until a median follow-up of 12 months [68]. Based on this meaningful and durable clinical benefit, nivolumab was approved by the FDA as a new treatment option for mCRC patients with MSI-H or dMMR who had disease progression after chemotherapy with fluoropyrimidine, oxaliplatin, and irinotecan.
dMMR/MSI-H also predict the efficacy of ICB combined therapy. Studies have confirmed that the efficacy of nivolumab combined with ipilimumab is better than that of nivolumab alone in small cell lung cancer (SCLC) and melanoma [69, 70]. In the nivolumab plus ipilimumab cohort of the CheckMate 142 trial, 119 mCRC patients with dMMR/MSI-H who progressed after two or more prior therapies were enrolled. The results showed that the ORR was 55% and that the DCR for more than 12 weeks was 80%; the 12-month PFS rate was 71%, and the corresponding OS rate was 85% regardless of PD-L1 expression, BRAF/KRAS mutation status, or clinical history of Lynch syndrome (see Table 2) [71]. A preliminary result from H-J J Lenz et al. demonstrated that nivolumab plus low-dose ipilimumab had an inspiring and promising benefit as a first-line therapy for mCRC patients with dMMR/MSI-H. The ORR and DCR were 60% (27/45) and 84% (38/45), respectively. The PFS rate was 78%, and the OS rate was 83% at 12 months [72]. M Chalabi et al. conducted an exploratory phase II trial to investigate nivolumab plus ipilimumab as a neoadjuvant therapy in resectable, early-stage colon cancers with dMMR and pMMR. Seven dMMR and 8 pMMR patients were enrolled. A major pathological response was observed in 100% of the dMMR patients, but no response was observed in pMMR patients [73]. However, these trials were somewhat limited by the lack of random grouping and small sample size, and thus, further investigation is urgent and necessary (see Table 3).
Table 2
The relationship between ICB and dMMR/MSI-H
Author/year
Cancer type
N
Protocol
Results
PFS
OS
Le DT [65]
2015
dMMR CRC
41
Pembrolizumab 10 mg/kg, q14d, 20 weeks
ORR 40%
20-week PFS, 78%; mPFS: NR
mOS: NR
pMMR CRC
ORR 0%
20-week PFS, 11%; mPFS: 2.2 months
mOS, 5.0 months
dMMR non-CRC
ORR 71%
20-week PFS, 67%; mPFS: 5.4 months
mOS: NR
pMMR CRC
25
ORR 0%
DCR16%
mPFS, 2.4 months
mOS, 6 months
Le DT [30]
2017
12 tumors with dMMR
86
Pembrolizumab 10 mg/kg, q14d, 2 years
ORR 53%
DCR 66%
12-month PFS, 64%
24-month PFS, 53%;mPFS: NR
12-month OS, 76%
24-month OS, 64%;mOS: NR
Diaz LA [66]
2017
Multiple types of solid tumors
77
Pembrolizumab 200 mg, q3w, 35 cycles
ORR 38%
DCR 58%
6-month PFS, 45%
mPFS, 4.3 months
6-month OS, 73%;mOS: NR
D Le [67]
2018
MMR/dMMR CRC
63
Pembrolizumab 200 mg, q3w, 35 cycles
ORR 28%
DCR 51%
6-month PFS, 43%
12-month PFS, 41%;mPFS, 4.1 months
6-month OS, 87%
12-month OS, 76%;mOS: NR
Overman MJ [68]
2017
dMMR/MSI-H mCRC
74
Nivolumab 3 mg/kg, q2w, until PD
ORR 31%
DCR 69% (≥ 12 weeks)
12-month PFS, 50%
mPFS, 14.3 months
12-month OS, 73%;mOS: NR
Overman MJ [71]
2018
dMMR/MSI-H mCRC
119
Nivolumab 3 mg/kg + ipilimumab 1 mg/kg, q3w, 4 doses, followed by nivolumab 3 mg/kg, q2w, until PD
ORR 55%
DCR 80%
9-month PFS, 76%
12-month PFS, 71%;mPFS: NR
9-month OS, 87%
12-month OS, 85%;mOS: NR
H-J J Lenz [72]
2018
dMMR/MSI-H mCRC
45
Nivolumab 3 mg/kg, q2w + ipilimumab 1 mg/kg, q6w, until PD (as first-line treatment)
ORR 60%
DCR 84%
12-month PFS, 78%;mPFS: NR
12-month OS: 83%;mOS: NR
M Chalabi [73]
2018
dMMR early-stage CC
pMMR early-stage CC
7
8
Nivolumab 3 mg/kg, d1, d15 + ipilimumab 1 mg/kg, d1
mPR 100%
mPR 0%
NA
NA
Abbreviations: dMMR mismatch repair deficient, CRC colorectal cancer, mCRC metastatic colorectal cancer, CC colon cancers, pMMR mismatch repair proficient, ORR objective response rate, PFS progression-free survival, mPFS median progression-free survival, NR not reached (the responses were durable and the results were not reached until the end of follow-up), mOS median overall survival, DCR disease control rate, OS overall survival, mCRC metastatic colorectal cancer, MSI-H microsatellite instability-high, PD disease progression, mPR major pathological response, NA not available
Table 3
Ongoing clinical trials evaluating ICB therapies in dMMR/MSI-H tumors
Clinical trial
Phase
Drug treatment
Drugs
Tumor type
Current status
NCT03150706
II
Avelumab
Anti-PD-L1 mAb
Previously treated dMMR/MSI-H or POLE-mutated mCRC
Ongoing
NCT03435107
II
Durvalumab
Anti-PD-L1 mAb
Previously treated dMMR/MSI-H or POLE-mutated mCRC
Ongoing
NCT02983578
II
Durvalumab (MEDI4736) + AZD9150
Anti-PD-L1 mAb + Antisense STAT3
dMMR CRC, NSCLC, and advanced pancreatic cancer
Ongoing
NCT02997228
III
Atezolizumab + mFOLFOX6 + bevacizumab versus mFOLFOX6 + bevacizumab versus atezolizumab
Anti-PD-L1 mAb
dMMR mCRC
Ongoing
NCT02912559
III
Atezolizumab + standard chemotherapy* versus standard chemotherapy*
Anti-PD-L1 mAb
dMMR stage III resected CRC
Ongoing
NCT03257163
II
Pembrolizumab + capecitabine + radiation therapy
Anti-PD-1 mAb
dMMR and Epstein-Barr virus positive GC
Ongoing
NCT02563002
III
Pembrolizumab versus standard therapy**
Anti-PD-1 mAb
dMMR/MSI-H stage IV CRC
Not recruiting
NCT03236935
Ib
Pembrolizumab + L-NMMA
Anti-PD-1 mAb + nitric oxide synthase inhibitor
dMMR/MSI-H cancer, melanoma, NSCLC, HNSCC, classic HL, and urothelial carcinoma
Ongoing
NCT03607890
II
Nivolumab + relatlimab
Anti-PD-1 mAb + anti-LAG-3 mAb
MSI-H solid tumors refractory to prior PD-(L)1 therapy
Not recruiting
NCT02992964
I/II
Nivolumab
Anti-PD-1 mAb
Pediatric patients with hypermutant cancers, including biallelic MMR syndrome
Ongoing
NCT03241745
II
Nivolumab
Anti-PD-1 mAb
dMMR/MSI-H/hypermutated uterine cancer
Ongoing
NCT02060188
II
Nivolumab versus nivolumab + ipilimumab or nivolumab + ipilimumab + cobimetinib or nivolumab + BMS-986016 or nivolumab + daratumumab
Anti-PD-1 mAb + Anti-CTLA-4 mAb + MEK inhibitor + anti-LAG-3 mAb + anti-CD38 mAb
dMMR/pMMR/MSI-H/MSI-L/MSS CRC
Ongoing
Abbreviations: ICBs immune checkpoint blockades, PD-L1 programmed death-ligand 1, mAb monoclonal antibody, dMMR mismatch repair deficient, pMMR mismatch repair proficient, MSI-H microsatellite instability-high, MSI-L microsatellite instability-low, MSS microsatellite stable, CTLA-4 cytotoxic T-lymphocyte protein 4, mCRC metastatic colorectal cancer, CRC colorectal cancer, NSCLC non-small cell lung cancer, GC gastric cancer, HNSCC head and neck squamous cell carcinoma, HL Hodgkin lymphoma, mFOLFOX 6 denotes fluorouracil plus leucovorin calcium and oxaliplatin
*Standard chemotherapy denotes fluorouracil plus leucovorin calcium and oxaliplatin
**Standard therapy denotes mFOLFOX6 or mFOLFOX6 plus bevacizumab, or mFOLFOX6 plus cetuximab, or FOLFIRI, or FOLFIRI plus bevacizumab, or FOLFIRI plus cetuximab (FOLFIRI denotes irinotecan plus leucovorin and fluorouracil)
Why does dMMR reflect the efficacy of immunotherapy? Le DT et al. reported that an average of 1782 somatic mutations per tumor and 578 potential neoantigens were found in dMMR tumors, compared with 73 mutations and 21 neoantigens in pMMR tumors by exome sequencing (P = 0.007). Higher numbers of somatic mutations and neoantigens were correlated with better responses and longer PFS. Furthermore, dMMR tumors have a dense infiltration of CD8+ TILs, which induce a better and more durable response [65]. In view of the abovementioned results, routine testing of the MMR status should be considered in the clinic regardless of tumor origin, which might bring an unexpected benefit to the patients with dMMR/MSI-H tumors. In summary, dMMR/MSI-H tumors treated with ICB demonstrate a durable response and a sustained survival benefit, and the combination of ICB therapies could further improve outcomes in these patients. The survival benefit remains to be explored in patients with refractory metastatic cancers. Table 3 shows the ongoing clinical trials evaluating ICB therapies in dMMR/MSI-H tumors. dMMR tumors are more frequent in early-stage cancers than in metastatic cancers, and therefore, the efficacy of immunotherapy in early-stage tumors is worthy of further investigation.

Relationship between dMMR/MSI-H and other immune biomarkers

PD-1/PD-L1 checkpoints have important functions in maintaining immune-tolerance and preventing effective antitumor immunity. The numbers of PD-1+ TILs are significantly different among various cancer types and range from 0% in extraskeletal myxoid chondrosarcomas and uterine sarcoma to 93% in ovarian cancer [49, 74]. The positive rates of membranous PD-L1 expression vary from 50–97% in NSCLC, bladder carcinoma, renal cell carcinoma, endometrial cancer, melanoma, and sarcomas, but its expression is less than 10% in liver cancer and is absent in Merkel cell carcinoma [74]. PD-L1+ expression has been used as common stratification factor in many clinical trials and used as an important biomarker to guide the ICB therapy in clinical practice [61, 7580]. PD-L1+ expression is closely related to dMMR/MSI-H status. Both Gatalica and Inaguma reported that the proportion of PD-L1+ expression in dMMR/MSI-H CRC was significantly higher than in pMMR/MSS CRC [74, 81]. Kim ST et al. reported that PD-L1+ expression was 38.9% in MLH1/MSH2-negative solid tumors compared with 15.2% in pMMR tumors [82]. In other studies, the PD-L1+ rate varied from 12.1–35.2% in pMMR GC and from 46.7–60.0% in dMMR GC (P < 0.01) [83, 84] (see Table 4). Lee SJ et al. [85] showed that the expression of PD-L1, lymphocyte-activation gene 3 (LAG3), and indolamine 2′3′-dioxygenase 1 (IDO1) in TILs was 68.6%, 13.5%, and 28.1%, respectively, in 89 patients with MSI-H colon cancer. A higher number of mutations in DNA coding sequences in MSI-H tumors have more potential to stimulate the host to generate neoantigens and trigger immune activation [65, 81]. Llosa NJ et al. indicated that the levels of PD-1, PD-L1, CTLA-4, LAG3, and IDO1 have been found to be significantly upregulated to balance the activated immune response in MSI-H tumors compared with MSS tumors [86]. Therefore, blocking the interaction between PD-1/PD-L1 and other immune negative regulatory pathways may increase activation of Th1 cytotoxic immune responses and significantly enhance the ability of the host to kill cancer cells, especially in dMMR/MSI-H tumors [87].
Table 4
The relationship between PD-L1 and dMMR/pMMR
Author/year
Tumor
N
PD-L1+ (%)
dMMR (%)
PD-L1+ in dMMR tumors (%)
PD-L1+ in pMMR tumors (%)
P
The impact of PD-L1 or dMMR on survival
Gatalica Z [74]
2014
CC
87
20.7
31.0
38.0
13.0
0.02
NA
Inaguma S [83]
2016
CRC
506
NA
NA
44.7
6.8
< 0.01
NA
Inaguma S [83]
2016
GC
180
NA
NA
46.7
12.1
< 0.01
NA
Kim ST [82]
2017
Advanced GI, GU, and others
430
16.5 in all, 28.6 in melanoma,
22.4 in GC, 20.9 in CRC,
12.5 in BTC, 7.1 in GU,
6.7 in HCC, 0.0 in pancreatic cancer and sarcoma
4.5 in all
7.1 in GC
6.7 in HCC
4.4 in CRC
38.9
15.2
< 0.01
P = 0.535 in GC
P = 0.231 in mCRC
P = 0.508 in sarcoma
Mills AM [26]
2018
Breast carcinoma
245
12.0 in all
32.0 in TNDC
0.04
100.0
NA
NA
NA
Wang L [84]
2018
GC
550
37.3
8.2
60.0
35.2
< 0.01
NA
Abbreviations: N number, PD-L1 programmed death-ligand 1, dMMR mismatch repair deficient, pMMR mismatch repair proficient, NA not available, CC colon cancer, CRC colorectal cancer, GC gastric cancer, GI gastrointestinal cancer, GU genitourinary cancer, BTC biliary tract cancer, HCC hepatocellular carcinoma, mCRC metastatic colorectal cancer, TNDC triple negative ductal carcinoma
TMB is another promising predictor for anti-PD-1/PD-L1 immunotherapy compared to dMMR/MSI-H, and the relevant content has been widely studied in lung cancer [8892], melanoma [93], bladder cancer [94], and others. The exploratory subgroup analyses in the CheckMate 026 trial demonstrated that patients with previously untreated stage IV or recurrent NSCLC obtained significant ORR and mPFS benefits from nivolumab than platinum-based chemotherapy in the high TMB (TMB ≥ 243 mutations) group (ORR 47% vs 28%; mPFS 9.7 months vs 5.8 months). No significant clinical benefit was observed in regards to ORR or PFS in the low (0 < TMB < 100 mutations) or medium TMB (100 ≤ TMB < 243 mutations) group [88]. In the CheckMate 568 trial, the ORR was 4%, 10%, 44%, and 39% when the TMB cutoffs were < 5, < 10, ≥ 10, and ≥ 15 mut/Mb in NSCLC patients treated with nivolumab plus ipilimumab as a first-line therapy. Subsequently, TMB ≥ 10 mut/Mb was regarded as the criteria to differentiate the high TMB and low TMB cohorts in the CheckMate 227 trial [89]. The prospective phase III trial confirmed that nivolumab plus ipilimumab resulted in a significantly longer PFS and higher ORR only in high TMB patients with stage IV or recurrent NSCLC compared with chemotherapy (mPFS 7.2 months vs 5.5 months; ORR 45.3% vs 26.9%) [90]. According to the abovementioned trials, the National Comprehensive Cancer Network guidelines firstly recommended that TMB was an emerging biomarker to identify patients with NSCLC for nivolumab or nivolumab plus ipilimumab in version 1 of 2019 [80]. The CheckMate 032 trial demonstrated better clinical benefit in high TMB (TMB ≥ 248 mutations) patients with SCLC [91]. Robert M. Samstein et al. reported that higher TMB was significantly associated with better OS in 1662 patients treated with either anti-CTLA-4 or anti-PD-1 therapies in diverse cancer types. These studies provided robust evidence for the predictive power of TMB in guiding the application of ICB [95]. TMB is commonly detected through tissue, and blood detection is the substitute due to the lack of tissue. Gandara et al. showed that high blood-based TMB (bTMB ≥ 16 mut/Mb) levels were positively associated with improved PFS and OS in NSCLC patients treated with atezolizumab versus docetaxel as a second-line or more-line choice [92]. More recently, Zhijie Wang et al. found that bTMB can be well estimated and measured by a cancer gene panel (CGP) named NCC-GP150 in patients with NSCLC. In clinical validation, 50 patients with NSCLC with high bTMB (≥ 6 mut/Mb) was associated with prolonged mPFS and higher ORR than patients with low bTMB (< 6 mut/Mb) (mPFS not reach vs 2.9 m; ORR 39.3% vs 9.1%) when treated with anti-PD-1/PD-L1 therapies [96] (see Table 5).
Table 5
TMB predicts the efficacy of ICB therapy
Clinical trial
Phase
Drug
TMB (mut/Mb)
Tumor
N
Response
PFS
OS
CheckMate026 [88]
III
Nivolumab
H ≥ 243
NSCLC
47
ORR 47%
*mPFS, 9.7 moths
1-year OS, 64%; mOS, 18.3 months
Platinum-based CT
60
ORR 28%
*mPFS, 5.8 months
1-year OS, 60%; mOS, 18.8 months
Nivolumab
L 0 to 99 and M 100 to 242
111
ORR 23%
mPFS, 4.1 months
1-year OS, 54%; mOS, 12.7 months
Platinum-based CT
94
ORR 33%
mPFS, 6.9 months
1-year OS, 53%; mOS, 13.2 months
CheckMate568 [89]
II
Nivolumab + ipilimumab
< 5,
5–10,
10–15,
≥ 15
NSCLC
288
ORR 4%, ORR 10%, ORR 44%, ORR 39%
NA
NA
CheckMate227 [90]
III
Nivolumab + ipilimumab
≥ 10
NSCLC
139
ORR 45.3%
1-year DoR 68%
*1-year PFS, 42.6%
*mPFS, 7.2 months
NA
Platinum doublet CT
160
ORR 26.9%
1-year DoR 25%
*1-year PFS, 13.2%
*mPFS, 5.5 months
Nivolumab + ipilimumab
< 10
   
mPFS, 3.2 months
 
Platinum doublet CT
    
mPFS, 5.5 months
 
CheckMate 032 [91]
Exploratory
Nivolumab + ipilimumab
H ≥ 248
SCLC
26
ORR 46.2%
1-year PFS, 30.3%
mPFS, 7.8 months
1-year OS, 62.4%; mOS, 22.0 months
Nivolumab
47
ORR 21.3%
1-year PFS, 21.2%
mPFS, 1.4 months
1-year OS, 35.2%; mOS, 5.4 months
Nivolumab + ipilimumab
M 143 to 247
25
ORR 16.0%
1-year PFS, 8.0%
mPFS, 1.3 months
1-year OS, 19.6%; mOS, 3.6 months
Nivolumab
44
ORR 6.8%
1-year PFS, 3.1%
mPFS, 1.3 months
1-year OS, 26.0%; mOS, 3.9 months
Nivolumab + ipilimumab
L 0 to 143
27
ORR 22.2%
1-year PFS, 6.2%; mPFS, 1.5 months
1-year OS, 23.4%; mOS, 3.4 months
Nivolumab
42
ORR 4.8%
1-year PFS, NC;mPFS, 1.3 months
1-year OS, 2.1%; mOS, 3.1 months
POPlAR [92]
Training
Atezolizumab
H-bTMB ≥ 16
NSCLC
25
NA
*mPFS, 4.2 months
*mOS, 13.0 months
Docetaxel
38
*mPFS, 2.9 months
*mOS, 7.4 months
OAK [92]
Validation
Atezolizumab
H-bTMB ≥ 16
NSCLC
77
ORR 21%
*PFS (HR 0.65, 95% CI 0.47–0.92; P = 0.013)
*mOS, 13.5 months
Docetaxel
81
ORR 10%
*mOS, 6.8 months
Atezolizumab
bTMB < 16
NSCLC
216
ORR 13%
PFS (HR 0.98, 95% CI 0.80–1.2)
OS (HR 0.65, 95% CI 0.52–0.81)
Docetaxel
  
209
ORR 12%
Zhijie W et al. [96]
 
Anti-PD-1/PD-L1 therapies
bTMB ≥ 6
bTMB < 6
NSCLC
28
22
*ORR 39.3%
*ORR 9.1%
*mPFS: NR
*mPFS, 2.9 months
 
Abbreviations: N number, ICBs immune checkpoint blockades, PD-L1 programmed death ligand 1, PD-1 programmed death-1, CTLA-4 cytotoxic T lymphocyte antigen-4, mAb monoclonal antibody, NSCLC non-small cell lung cancer, SCLC small-cell lung cancer, CT chemotherapy, ORR objective response rate, DoR duration of response, PFS progression-free survival, mPFS median progression-free survival, OS overall survival, mOS median overall survival, TMB tumor mutation burden, H high, M medium, L low, bTMB blood-based tumor mutation burden, mut mutation, mut/Mb mutation per megabase, Ref reference, vs versus, NR not reached, NA not available
*Denotes the difference was statistically significant
Compared with dMMR/MSI-H or PD-1/PD-L1 expression, the TMB is emerging as a more accurate, comprehensive, and compelling potential biomarker that could predict the efficacy of ICB therapy. However, there is no consensus on the measuring of TMB status [95]. In several studies, most patients with MSI-H had high TMB levels; however, not all patients with high TMB levels had dMMR/MSI-H status or high PD-L1 expression [88, 97, 98]. Rizvi H et al. indicated that there was no correlation between PD-L1 and TMB status in patients with NSCLC treated with ICB (r = 0.1915, P = 0.08). Patients with high-TMB and positive PD-L1 expression had the highest rate of durable clinical benefit than that with only one or neither variable presence (50% vs. 18.2–35.5%) [98]. Fabrizio DA et al. demonstrated that 99.7% of CRC patients with MSI-H had high TMB status (6.3–746.9 mut/Mb); meanwhile, 97.0% of CRC patients with MSS were low TMB (0.0–10.8 mut/Mb) in a large population. Although there was a high consistency between MSI-H and high TMB status in CRC, 2.9% (163/5702) of patients with MSS were still considered as high TMB [97]. Zachary R et al. analyzed 100,000 human cancer genomes to reveal the landscape of TMB and found that nearly 83% of tumor samples with MSI-H showed high TMB status (TMB > 20 mut/Mb), whereas only 16% of tumor samples with high TMB was MSI-H and nearly 84% were classified as MSS [99]. ICB therapy is not recommended for patients with MSI-L/MSS, but these patients might have high TMB and could still benefit from ICB therapy [99]. The co-occurrence of high TMB and MSI-H varied among diverse cancer types, and they usually come together in gastrointestinal cancers, but are rarely consistent in lung cancer or melanoma in which the presence of high TMB is common [99]. Comprehensive analysis of dMMR/MSI-H, PD-L1, and TMB or a multivariable predictive model composed of 9 exome parameters (the DNA repair pathway status, the WNT pathway status, the number of TCR clones, the number of neoantigens, the HLA*A*1 and HLA*A*24 status, and the fractions of signatures 1A, 1B, and 6) resulted in greater predictive power and may allow for the optimal usage of ICB therapy [88, 98, 100, 101].

Conclusions and prospects

Immunotherapy has dramatically changed the therapeutic landscape of multiple tumors and has boosted enthusiasm regarding cancer treatment. Recent positive results from clinical trials of ICB therapies alone or in combination for “difficult-to-treat” dMMR/MSI-H tumors have led to great hope for immunotherapy application in this specific population. dMMR/MSI-H has been approved by the FDA as an indication of ICB for metastatic cancers, irrespective of the cancer types, presumably due to the enhanced immune response through the presence of increased somatic mutations and “nonself” neoantigens in these tumors. The novel use of ICB therapies as first-line or neoadjuvant treatments in dMMR/MSI-H tumors may have the potential to expand the indications. dMMR/MSI-H has its unique advantages compared with PD-L1, TMB, TILs, and other new predictors. Despite encouraging results of ICB by recognizing dMMR/MSI-H, only a fraction of patients typically have the dMMR/MSI-H features, and some sensitive patients still cannot be distinguished. Comprehensive analysis of multiple markers will provide the optimal strategy to identify sensitive patients to ICB therapy in the near future.

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Additional file 1: Figure S1. Statistically significant survival analysis of different tumors between the dMMR group and the pMMR group. (PPTX 379 kb)
13045_2019_738_MOESM1_ESM.pptx
Additional file 2: Figure S2. Survival analysis of tumors with or without MMR gene alterations. (PPTX 2269 kb)
13045_2019_738_MOESM2_ESM.pptx
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