Erschienen in:
28.10.2016 | Original Article
Mitophagy is increased during erythroid differentiation in β-thalassemia
verfasst von:
Limei Wu, Wei Xu, Luhong Xu, Qian Kong, Jianpei Fang
Erschienen in:
International Journal of Hematology
|
Ausgabe 2/2017
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Abstract
Mitophagy is a selective degradation of mitochondria, which also plays a critical role in hematopoiesis. However, it is unclear what role, if any, this process plays in the pathogenesis of β-thalassemia. To determine the role of mitophagy in β-thalassemia, CD34+ hematopoietic progenitor cells (HPCs) were isolated from peripheral blood of β-thalassemia patients and healthy controls and differentiated into erythrocytes. We found that the ratio of mitochondrial membrane depolarization was significantly increased, and that mitochondria co-localize with lysosomes at a higher level in β-thalassemia compared with control. Furthermore, the expression of LC3-II and Nix, as well as degradation of p62, in β-thalassemia was higher than in the control. In sum, our data suggest that selective mitophagy is enhanced during erythrocyte differentiation in β-thalassemia.