Background
Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction with chronic airway inflammation and emphysematous changes in the lung parenchyma [
1,
2]. The cardinal symptoms of COPD are dyspnea and/or fatigue, which may result from ventilatory constraints, pulmonary gas exchange abnormalities, peripheral muscle dysfunction, cardiac dysfunction, or combination of the above. COPD is also characterized by persistent chronic inflammation that may extend beyond the pulmonary system, resulting in a state of persistent low grade systemic inflammation [
2,
3] which has been implicated in various co-morbidities of COPD [
4,
5]. These extrapulmonary manifestations contribute to morbidity and mortality in COPD patients.
Several systemic inflammatory markers or cytokines including C-reactive protein (CRP), IL-6, IL-8, and tumor necrosis factor (TNF)-α have been associated with COPD risk, COPD mortality, COPD exacerbations, or lung function decline [
6‐
9]. Serum CRP may provide prognostic information about morbidity and mortality in mild to moderate COPD [
7,
8] with relationships between CRP, IL-6, exercise tolerance, and health status [
8,
10]. Systemic IL-6 levels are inversely correlated with FEV
1, and quadriceps strength is independently associated with reduced exercise tolerance [
11,
12]. IL-8 and TNF-α may be important signaling molecules for neutrophil activation. Increased sputum TNF-α and IL-8 levels have been reported during exacerbations of COPD [
13]. Elevated levels of IL-8 have been found in the bronchoalveolar lavage fluid of smokers and COPD patients that correlated positively with neutrophil counts [
14]. Circulating levels of IL-8 are elevated during acute COPD exacerbations [
15], which contribute to a reduction in health-related quality of life [
16]. TNF-α and IL-6 are acute phase proteins known to upregulate the synthesis of CRP, and fibrinogen [
17]. The distance walked in the 6-minute walk test was inversely related to serum CRP, IL-6 and IL-8 levels in COPD patients [
9]. Taken together, these data suggest that systemic inflammation related to COPD may in part be related to the reduced muscle strength and exercise tolerance observed in COPD patients.
Exercise training is the cornerstone of comprehensive rehabilitation programs in COPD [
1]. It improves skeletal muscle oxidative capacity and efficiency with reduced alveolar ventilation at a given work rate [
18,
19], leading to tolerance of a heavier work load with less dyspnea [
20]. Endurance exercise training is clinically efficacious [
21]. A recent review supports the notion that mobile technology is a promising way of delivering health services and enhances the exercise training program of pulmonary rehabilitation [
1]. Most pulmonary rehabilitation programs are hospital-based and rely on regular supervision and monitoring to achieve persistent and optimal physiological benefits. However, compliance is a major stumbling block [
22]. We have developed a home-based exercise training program for stable COPD patients by asking them to walk at a speed controlled by the tempo of music from a program installed on a mobile phone [
23]. This system provides an efficient home endurance exercise training program with good compliance and clinical outcomes in patients with moderate-to-severe COPD [
23]. We further explored whether the circulating levels of inflammatory markers, such as CRP, IL-6, TNF-α, and IL-8 will be reduced in patients with COPD undergoing a mobile-phone assisted home-based exercise training program [
23].
Discussion
We demonstrated that COPD patients, under the assistance of a mobile phone, were able to perform a home-based, individualized endurance exercise training program at an intended walking speed controlled by a pre-set tempo of music. During the six month follow-up period of home-based exercise training, exercise capacity, strength of limb muscles and inflammatory biomarkers of CRP and IL-8 were improved in the group under mobile phone guidance, but not in control group. In control group, who did not benefit from regular home-exercise training in the COPD patients, the level of proinflammatory cytokines of IL-6 and TNF-α remained elevated at 3 and 6 months. Our work indicates for the first time that long-term home exercise training using an individualized design and adjusted training protocol can reduce the inflammatory biomarkers, CRP and IL-8, which is likely the result of an improvement in systemic inflammation.
CRP is an acute-phase reactant that can be upregulated sensitively during systemic inflammation, and is associated with muscle mass, strength, physical function, and disability [
27]. CRP levels can be reduced in healthy subjects receiving 9 months of endurance exercise training [
28]. Increased concentrations of CRP and IL-6 were associated with impaired physical function in older adults with COPD [
29]. In the control group, there was a gradual increase in levels of proinflammatory cytokines, IL-6, TNF-α and CRP in patients who performed home exercise training of free walk. COPD patients are likely to become de-conditioned gradually due to dyspnea and systemic inflammation [
30], and may therefore show significant cytokine response at low levels of activity. Regular exercise may induce anti-inflammatory effects [
31]. Our previous study showed that under the mobile-phone assisted home exercise training program has improved exercise capacity, breathlessness, quality of life, inspiratory capacity and air-trapping in patients with COPD [
23]. In this study, we extend further the benefits of this program by showing that the strength of upper and lower limb muscles in COPD patients increased after 2 or 3 month of regular exercise. Thus, a mobile phone-based home exercise training program can provide an effective training for COPD patients. Long-term exercise training can improve systemic inflammatory biomarkers in patients with COPD.
The presence of systemic inflammation has been suggested by studies [
32,
33] showing systemic oxidative stress, the activation of circulating neutrophils and lymphocytes, and increased plasma levels of TNF-α. The presence of oxidative stress in the airways of smokers and patients with COPD has been shown by increased products of lipid peroxidation and altered antioxidant status [
34]. Transcription factors such as nuclear factor-κB (NF-κB) and activator protein-1 (AP-1), which are oxidant-sensitive, are important for gene transcription of the inflammatory cytokines, and associated with upregulated airway inflammation in COPD. Proinflammatory cytokines, such as IL-8, TNF-α and IL-6 [
34,
35] are oxidant-sensitive proteins. For example, IL-8 gene has both NF-kB and AP-1 binding sites in the promoter region that regulates its transcriptional activation [
36]. Exercise training improves skeletal muscle oxidative capacity and efficiency that leads to less alveolar ventilation for a given work rate [
18,
19]. Patients can tolerate a heavier work load with less dyspnea on exercise [
37]. The lower limb muscles play an important role in exercise training programs because the quadriceps muscles are the major muscles for patients’ mobilization [
38]. In our study, the upper limb muscles also benefit from the paced-walk exercise training program, possibly related to the coordinated training high-intensity exercise with swing and balance of upper limbs.
Our results demonstrated that the serum levels of TNF-α and IL-6 were increased in the control group. The effects of peripheral muscle training on specific systemic inflammatory mediator levels (TNF-α, IL-6 and CRP) have been proposed to be associated with muscle dysfunction in COPD [
39,
40]. Endurance exercise training in COPD can induce peripheral muscle adaptation and modification by factors regulating skeletal muscle hypertrophy and regeneration [
41], but in the absence of a decrease in systemic or local muscle TNF-α and IL-6, similar to our results in the mobile phone group. In addition, people with COPD who walked the most had the lowest plasma CRP and IL-6 levels [
42]. The systemic inflammatory process, evaluated by serum IL-6 and TNF-α which may result from pulmonary inflammation and oxidative stress or hypoxia [
40,
43], seems to be persistent and progressive in COPD patients [
42,
44]. In our study, COPD patients in the control group may have less intensity of endurance walking training after stopping regular hospital-based pulmonary rehabilitation. They exhibited s raised plasma levels of TNF-α and IL-6 at 3 and 6 months compared to those in the mobile phone group. TNF-α and IL-6 are acute phase proteins known to upregulate the synthesis of CRP and fibrinogen [
17]. TNF-α activates the transcription factor, nuclear factor-κB (NF-κB), that switches on transcription of the IL-8 gene and increases IL-8 release from the airway epithelium and neutrophils [
36]. Our results also showed a significant increase in serum levels of CRP (at 3 and 6 months) and IL-8 (at 6 months) compared to the mobile phone group. These results provide the conceptual basis that walking leads to a reduction in systemic inflammation in COPD patients.
In the control group, one would expect that the walking distance may not be going up at the same rate as the intervention group, but would hopefully at least remain static than decline at 6 months. This finding had been reported in the control group of Wijkstra et al [
45]. One possibility is that all subjects in our control group received regular hospital-based rehabilitation of bicycling training once per week, which can successfully maintain functional exercise capacity [
46]. Without a supervised intervention, subjects of control group may decrease the time or intensity of home walking program. Therefore, their incremental shuttle walk distance only declined gradually. 6MWT is a self-paced walking test of fixed distance, while ISWT is a paced test of an indefinite duration. The presence of quadriceps muscle fatigue was more frequent and more pronounced after cycling than after walking. Another possibility is that the incremental shuttle walk in our study is more responsive to the effects of pulmonary rehabilitation than 6 minute walk test (6MWT) [
47]. In another study [
48], the walking distance appear to decline by twelve months after stopping pulmonary rehabilitation program.
Exercise training is the cornerstone of pulmonary rehabilitation [
1,
11]. High intensity training significantly improved patients’ exercise capacity and physiologic adaptation during endurance training [
49]. COPD patients presented with increased pulmonary and systemic oxidative stress after simple exercise compared with healthy control subjects [
50]. Unplanned exhausted exercise in COPD patients may lead to fatigue and contribute to exercise limitation related to the excessive load placed on inspiratory muscles, and deteriorate systemic inflammation [
51]. Through well-designed evaluation of patients’ exercise capability, our mobile phone based program provides a real-time monitoring about patients’ daily exercise training, and dyspnea sensation. The central monitoring of patients’ physical activity offers the opportunity for early intervention and management in COPD patients to prevent exacerbation of disease. This specific computer-based monitoring strategy may further reduce the medical costs through the planned exercise training which reduced systemic inflammation, and improved patients’ adherence through daily communication.
A randomized clinical trial showed that weekly telephone contacts and monthly supervised rehabilitation sessions produced only modest effects in maintaining improvements in exercise tolerance and ratings of overall health status [
52]. The exercise tolerance declined gradually in the usual care group. In contrast, distance walked on the endurance shuttle waking test (ESWT) significantly improved either in the hospital or community rehabilitation groups. There was no difference in exercise tolerance between hospital or community groups with the telephone and no-telephone groups [
53]. Our work showed a greater improvement of exercise in COPD patients by mobile phone home-based exercise program. Effectiveness of intervention or contact or combined with an optimal method, such as cell phone, for incorporating rehabilitation strategies may have been critical in motivating participants to continue exercise.
The present study indicates that COPD patients can substantially benefit from rehabilitation with lower systemic inflammation, even though hospital based exercise training once per week. Several home-based rehabilitation programs have been developed with proven clinical benefits in quality of life and exercise tolerance [
54,
55]. In those programs for which walking freely for a period of time is recommended, it is more difficult to establish the walking speed necessary to achieve a training effect at home [
56]. The long-term adherence to exercise training is the critical factor in sustaining the clinical benefit in the home setting program. It declines when regular monitoring or supervision is removed [
57], like our control group with progressive elevation of cytokines. The current cell phone-assisted system may provide an efficient home-based exercise training program, due to its controlled exercise workload by pacing walking and web-based supervision of exercise performance and low-grade systemic inflammation.
The limitations of this study are its relatively small patient numbers, and short-term follow-up period. However, these results demonstrate that intensive daily exercise programs without undue exhaustion can be performed successfully on an out-patient basis through a mobile phone based communication. This tele-rehabilitation program combined the advantage of both the self-management strategies and the direct investigation from the health care groups via computer monitoring. Further longitudinal observation for outcomes which includes decline in pulmonary function, deterioration in muscle endurance, as well as the systemic inflammatory mediators may lead to useful measures to overcome detrimental changes in COPD patients.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
CHW performed the work of part of design, acquisition of data and drafting the article. PCC contributed to the acquisition of data, as well as part work of writing article. WCJ, LFC, TFS, and SCH contributed to the data collection and education of exercise training program for patients. HCL, CDH, and FTC worked on the acquisition and analysis of data. KFC contributed to study concept and design, interpretation of data, and revising the manuscript critically for important intellectual content. HPK took the substantial responsibility to main concept and design of this work and contributed to the analysis and interpretation of data and final approval of the final version. All authors read and approved the final manuscript.