Introduction
Methods
Animals and treatment
Cell culture
Parameters of liver damage
Plasminogen activator inhibitor 1 (PAI-1) ELISA
RNA isolation and real time RT-PCR
Immunohistochemical staining of inducible nitric oxide synthase, 4-hydroxynonenal protein adducts and 3-nitrotyrosine protein adducts in liver tissue
Statistical analyses
Results
Effect of moderate alcohol and beer consumption on body weight and liver status
Diet groups | ||||
---|---|---|---|---|
C-D | FFC | FFC + E | FFC + B | |
Daily energy intake (kJ) | 38.1 ± 0.9 | 37.4 ± 0.9 | 36.8 ± 1.0 | 39.7 ± 1.5 |
Alcohol intake (g/kg body weight) | – | – | 2.5 ± 0.1 | 2.5 ± 0.1 |
Absolute body weight (g) | 21.9 ± 0.5 | 22.3 ± 0.5 | 22.0 ± 0.2 | 22.5 ± 0.3 |
Absolute weight gain (g) | 3.3 ± 0.3 | 3.5 ± 0.4 | 3.6 ± 0.3 | 3.9 ± 0.2 |
Liver weight (g) | 1.1 ± 0.04 | 1.5 ± 0.03a,b | 1.4 ± 0.03a | 1.3 ± 0.1a |
Liver:body weight ratio (%) | 5.0 ± 0.1 | 6.7 ± 0.1a,b | 6.5 ± 0.1a,b | 5.9 ± 0.2a |
ALT (U/L) | 21.0 ± 2.0 | 46.0 ± 11.9 | 50.7 ± 9.2 | 21.7 ± 3.5 |
AST (U/L) | 41.7 ± 2.4 | 73.7 ± 17.2 | 87.1 ± 15.0 | 43.7 ± 2.9 |
Effect of moderate alcohol and beer consumption, respectively, on fasting blood glucose levels, glucose tolerance and markers of insulin signaling in liver tissue
Effect of moderate alcohol and beer consumption, respectively, on genes involved in regulating adiponectin (Adipoq) expression in visceral adipose tissue and adiponectin receptor 1 and 2 (Adipor1 and 2) mRNA expression in liver tissue
Diet groups | ||||
---|---|---|---|---|
C-D | FFC | FFC + E | FFC + B | |
Pparγ1 mRNA expression (% of control) | 100.0 ± 16.5 | 102.4 ± 30.6 | 99.6 ± 13.2 | 101.9 ± 10.4 |
Foxo1 mRNA expression (% of control) | 100.0 ± 26.5 | 143.6 ± 26.0 | 111.0 ± 13.6 | 118.3 ± 34.9 |
Sirt1 mRNA expression (% of control) | 100.0 ± 16.5 | 140.8 ± 36.8 | 99.6 ± 16.7 | 310.6 ± 98.1a,b |
Adipoq mRNA expression (% of control) | 100.0 ± 20.0 | 51.8 ± 10.8 | 76.6 ± 14.5 | 166.7 ± 47.0 |
Effect of ethanol and beer on Adipor1 and Adipor2 mRNA expression of murine monocytes (J774A.1)
Effect of moderate alcohol and beer consumption, respectively, on iNOS and 4-HNE protein adduct concentration and on markers of lipogenesis in liver tissue
Diet groups | ||||
---|---|---|---|---|
C-D | FFC | FFC + E | FFC + B | |
Fas mRNA expression (% of control) | 100.0 ± 24.8 | 132.9 ± 29.3 | 115.6 ± 26.2 | 136.0 ± 24.7 |
Srebp-1c mRNA expression (% of control) | 100.0 ± 17.7 | 151.1 ± 18.3 | 171.8 ± 28.1 | 155.2 ± 16.8 |
Acox1 mRNA expression (% of control) | 100.0 ± 11.4 | 117.3 ± 14.2 | 141.4 ± 10.5 | 131.4 ± 5.1 |