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15.09.2020 | Myocardial Infarction

Modulation of the Expression of Long Non-Coding RNAs H19, GAS5, and MIAT by Endurance Exercise in the Hearts of Rats with Myocardial Infarction

verfasst von: Saeideh Jafarinejad Farsangi, Farzaneh Rostamzadeh, Mozhgan Sheikholeslami, Elham Jafari, Mohammadreza Karimzadeh

Erschienen in: Cardiovascular Toxicology | Ausgabe 2/2021

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Abstract

Long non-coding RNAs (lncRNAs) have a critical role in the regulation of cardiovascular function. Dysregulation of lncRNAs is implicated in the progression of cardiovascular diseases including myocardial infarction (MI). Regarding the beneficial effects of exercise (Ex) on the improvement of MI, this study aimed to investigate the effects of post-MI Ex on the expression of MI-associated lncRNAs: H19, myocardial infarction association transcript (MIAT), and growth arrest specific 5 (GAS5). MI was induced by left anterior descending (LAD) coronary artery ligation in male Wistar rats. One week later, rats were exercised under a moderate-intensity protocol for 4 weeks. In the end, hemodynamic parameters and cardiac function indices were measured. Assessment of fibrotic areas and apoptosis was performed by Masson's trichrome staining and immunohistochemistry, respectively. Expression of genes was evaluated by real-time PCR. Ex significantly reduced the fibrotic areas (P < 0.05) and apoptosis and increased contractility indices (P < 0.01), and cardiac function (P < 0.05) in MI groups. The reduced expression of H19 (P < 0.01) in MI rats returned to normal levels by Ex. Ex significantly (P < 0.001) reduced the expression of MIAT and increased the expression of GAS5 (P < 0.01), which had changed in the hearts of rats with MI. The present study indicated the beneficial effect of Ex on the improvement of cardiac function and reduction of fibrosis in infarcted heart possibly through regulation of the expression of lncRNAs: H19, GAS5, and MIAT.

Pinaire, J., Aze, J., Bringay, S., Cayla, G., & Landais, P. (2019). Hospital burden of coronary artery disease: Trends of myocardial infarction and/or percutaneous coronary interventions in France 2009–2014. PLoS One, 14(5), e0215649.CrossRef

Vainio, L. E., Szabó, Z., Lin, R., Ulvila, J., Yrjölä, R., Alakoski, T., et al. (2019). Connective tissue growth factor inhibition enhances cardiac repair and limits fibrosis after myocardial infarction. JACC Basic to Translational Science, 4(1), 83–94.CrossRef

Xia, P., Liu, Y., & Cheng, Z. (2016). Signaling pathways in cardiac myocyte apoptosis. BioMed Research International, 2016, 9583268.PubMedPubMedCentral

Rippe, J. M. (2019). Lifestyle strategies for risk factor reduction, prevention, and treatment of cardiovascular disease. American Journal of Lifestyle Medicine, 13(2), 204–212.CrossRef

Sharma, S., Merghani, A., & Mont, L. (2015). Exercise and the heart: the good, the bad, and the ugly. European Heart Journal, 36(23), 1445–1453.CrossRef

Dibben, G. O., Dalal, H. M., Taylor, R. S., Doherty, P., Tang, L. H., & Hillsdon, M. (2018). Cardiac rehabilitation and physical activity: systematic review and meta-analysis. Heart (British Cardiac Society), 104(17), 1394–1402.

Anderson, L., Thompson, D. R., Oldridge, N., Zwisler, A. D., Rees, K., Martin, N., et al. (2016). Exercise-based cardiac rehabilitation for coronary heart disease. The Cochrane Database of Systematic Reviews., 2016(1), Cd001800.PubMedCentral

Fernandes, J. C. R., Acuna, S. M., Aoki, J. I., Floeter-Winter, L. M., & Muxel, S. M. (2019). Long non-coding RNAs in the regulation of gene expression: physiology and disease. Non-coding RNA, 5(1), 17.CrossRef

Chen, G., Wang, Z., Wang, D., Qiu, C., Liu, M., Chen, X., et al. (2013). LncRNADisease: a database for long-non-coding RNA-associated diseases. Nucleic acids research, 41(Database issue), D983–6.PubMed

10.

Jarroux, J., Morillon, A., & Pinskaya, M. (2017). History, discovery, and classification of lncRNAs. Advances in Experimental Medicine and Biology, 1008, 1–46.CrossRef

11.

Kim, D. K., Zhang, L., Dzau, V. J., & Pratt, R. E. (1994). H19, a developmentally regulated gene, is reexpressed in rat vascular smooth muscle cells after injury. The Journal of Clinical Investigation, 93(1), 355–360.CrossRef

12.

Choong, O. K., Chen, C. Y., Zhang, J., Lin, J. H., Lin, P. J., Ruan, S. C., et al. (2019). Hypoxia-induced H19/YB-1 cascade modulates cardiac remodeling after infarction. Theranostics, 9(22), 6550–6567.CrossRef

13.

Lee, J. H., Gao, C., Peng, G., Greer, C., Ren, S., Wang, Y., et al. (2011). Analysis of transcriptome complexity through RNA sequencing in normal and failing murine hearts. Circulation Research, 109(12), 1332–1341.CrossRef

14.

Wang, X. M., Li, X. M., Song, N., Zhai, H., Gao, X. M., & Yang, Y. N. (2019). Long non-coding RNAs H19, MALAT1 and MIAT as potential novel biomarkers for diagnosis of acute myocardial infarction. Biomedicine Pharmacotherapy Biomedecine Pharmacotherapie, 118, 109208.CrossRef

15.

Ishii, N., Ozaki, K., Sato, H., Mizuno, H., Saito, S., Takahashi, A., et al. (2006). Identification of a novel non-coding RNA, MIAT, that confers risk of myocardial infarction. Journal of Human Genetics, 51(12), 1087–1099.CrossRef

16.

Chen, L., Zhang, D., Yu, L., & Dong, H. (2019). Targeting MIAT reduces apoptosis of cardiomyocytes after ischemia/reperfusion injury. Bioengineered, 10(1), 121–132.CrossRef

17.

Wen, Q., Liu, Y., Lyu, H., Xu, X., Wu, Q., Liu, N., et al. (2017). Long noncoding RNA GAS5, which acts as a tumor suppressor via microRNA 21, regulates cisplatin resistance expression in cervical cancer. International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society, 27(6), 1096–1108.CrossRef

18.

Ji, J., Dai, X., Yeung, S. J., & He, X. (2019). The role of long non-coding RNA GAS5 in cancers. Cancer Management and Research, 11, 2729–2737.CrossRef

19.

Zhang, J. C., Xia, L., Jiang, Y., Wu, D. Q., Liu, S. C., Zhou, X. N., et al. (2019). Effect of lncRNA GAS5 on rats with acute myocardial infarction through regulating miR-21. European Review for Medical and Pharmacological Sciences, 23(19), 8573–8579.PubMed

20.

Hochman, J. S., & Bulkley, B. H. (1982). Expansion of acute myocardial infarction: an experimental study. Circulation, 65(7), 1446–1450.CrossRef

21.

Cai, M. X., Shi, X. C., Chen, T., Tan, Z. N., Lin, Q. Q., Du, S. J., et al. (2016). Exercise training activates neuregulin 1/ErbB signaling and promotes cardiac repair in a rat myocardial infarction model. Life Sciences, 149, 1–9.CrossRef

22.

Rostamzadeh, F., Najafipour, H., Yeganeh-Hajahmadi, M., Esmaeili-Mahani, S., Joukar, S., & Iranpour, M. (2017). Heterodimerization of apelin and opioid receptors and cardiac inotropic and lusitropic effects of apelin in 2K1C hypertension: role of pERK1/2 and PKC. Life Sciences, 191, 24–33.CrossRef

23.

Velkov, Z., Lolov, R., Lolov, V., Nicolov, N., & Todorova, M. (1986). Effect of captopril on some ventricular contractility indices in spontaneously hypertensive rats (SHR). The Japanese Journal of Physiology, 36(4), 815–820.CrossRef

24.

Weiss, J. L., Frederiksen, J. W., & Weisfeldt, M. L. (1976). Hemodynamic determinants of the time-course of fall in canine left ventricular pressure. The Journal of Clinical Investigation, 58(3), 751–760.CrossRef

25.

Tanriverdi, L. H., Parlakpinar, H., Ozhan, O., Ermis, N., Polat, A., Vardi, N., et al. (2017). Inhibition of NADPH oxidase by apocynin promotes myocardial antioxidant response and prevents isoproterenol-induced myocardial oxidative stress in rats. Free Radical Research, 51(9–10), 772–786.CrossRef

26.

Han, C. L., Ge, M., Liu, Y. P., Zhao, X. M., Wang, K. L., Chen, N., et al. (2018). Long non-coding RNA H19 contributes to apoptosis of hippocampal neurons by inhibiting let-7b in a rat model of temporal lobe epilepsy. Cell Death & Disease, 9(6), 617.CrossRef

27.

Zhou, X., Zhang, W., Jin, M., Chen, J., Xu, W., & Kong, X. (2017). lncRNA MIAT functions as a competing endogenous RNA to upregulate DAPK2 by sponging miR-22-3p in diabetic cardiomyopathy. Cell Death & Disease, 8(7), e2929.CrossRef

28.

Wu, N., Zhang, X., Bao, Y., Yu, H., Jia, D., & Ma, C. (2019). Down-regulation of GAS5 ameliorates myocardial ischaemia/reperfusion injury via the miR-335/ROCK1/AKT/GSK-3β axis. Journal of Cellular and Molecular Medicine, 23(12), 8420–8431.CrossRef

29.

Sawicki KT, Chang HC, Shapiro JS, Bayeva M, De Jesus A, Finck BN, et al. (2018). Hepatic tristetraprolin promotes insulin resistance through RNA destabilization of FGF21. JCI insight, 3(13), e95948.CrossRef

30.

Garza, M. A., Wason, E. A., & Zhang, J. Q. (2015). Cardiac remodeling and physical training post myocardial infarction. World Journal of Cardiology, 7(2), 52–64.CrossRef

31.

Chen C, Tang Y, Sun H, Lin X, Jiang B. (2019). The roles of long noncoding RNAs (lncRNAs) in myocardial pathophysiology. Bioscience Reports, 39(11), BSR20190966.CrossRef

32.

Qu, X., Du, Y., Shu, Y., Gao, M., Sun, F., Luo, S., et al. (2017). MIAT Is a pro-fibrotic long non-coding RNA governing cardiac fibrosis in post-infarct myocardium. Scientific Reports, 7, 42657.CrossRef

33.

Zhou, M., Zou, Y. G., Xue, Y. Z., Wang, X. H., Gao, H., Dong, H. W., et al. (2018). Long non-coding RNA H19 protects acute myocardial infarction through activating autophagy in mice. European Review for Medical and Pharmacological Sciences, 22(17), 5647–5651.PubMed

34.

Liu, L., An, X., Li, Z., Song, Y., Li, L., Zuo, S., et al. (2016). The H19 long noncoding RNA is a novel negative regulator of cardiomyocyte hypertrophy. Cardiovascular Research, 111(1), 56–65.CrossRef

35.

Zhang, Y., Hou, Y. M., Gao, F., Xiao, J. W., Li, C. C., & Tang, Y. (2019). lncRNA GAS5 regulates myocardial infarction by targeting the miR-525-5p/CALM2 axis. Journal of Cellular Biochemistry, 120(11), 18678–18688.CrossRef

36.

Hao, S., Liu, X., Sui, X., Pei, Y., Liang, Z., & Zhou, N. (2018). Long non-coding RNA GAS5 reduces cardiomyocyte apoptosis induced by MI through sema3a. International Journal of Biological Macromolecules, 120(Pt A), 371–377.

37.

Freedman, J. E., & Miano, J. M. (2017). Challenges and opportunities in linking long noncoding RNAs to cardiovascular, lung, and blood diseases. Arteriosclerosis, Thrombosis, and Vascular Biology, 37(1), 21–25.CrossRef

38.

Nielsen, M., Tehler, D., Vang, S., Sudzina, F., Hedegaard, J., Nordentoft, I., et al. (2013). Identification of expressed and conserved human noncoding RNAs. RNA (New York, NY), 20(2), 236–251.CrossRef

39.

Brannan, C. I., Dees, E., Ingram, R. S., & Tilghman, S. M. (1990). The product of the H19 gene may function as an RNA. Molecular and Cellular Biology, 10, 28–36.CrossRef

40.

Li, D., & Yang, M. (2017). Identification and characterization of conserved lncRNAs in human and rat brain. BMC Bioinformatics, 18(14), 31–38.

Titel: Modulation of the Expression of Long Non-Coding RNAs H19, GAS5, and MIAT by Endurance Exercise in the Hearts of Rats with Myocardial Infarction
verfasst von: Saeideh Jafarinejad Farsangi
Farzaneh Rostamzadeh
Mozhgan Sheikholeslami
Elham Jafari
Mohammadreza Karimzadeh
Publikationsdatum: 15.09.2020
Verlag: Springer US
Schlagwort: Myocardial Infarction
Erschienen in: Cardiovascular Toxicology / Ausgabe 2/2021
Print ISSN: 1530-7905
Elektronische ISSN: 1559-0259
DOI: https://doi.org/10.1007/s12012-020-09607-0

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Modulation of the Expression of Long Non-Coding RNAs H19, GAS5, and MIAT by Endurance Exercise in the Hearts of Rats with Myocardial Infarction

Abstract

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

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