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Journal of Assisted Reproduction and Genetics

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22.02.2016 | Embryo Biology

Molecular analysis of DNA in blastocoele fluid using next-generation sequencing

verfasst von: Yixin Zhang, Na Li, Li Wang, Huiying Sun, Minyue Ma, Hui Wang, Xiaofei Xu, Wenke Zhang, Yingyu Liu, David S. Cram, Baofa Sun, Yuanqing Yao

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 5/2016

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Abstract

Background

Preimplantation genetic testing (PGT) requires an invasive biopsy to obtain embryonic material for genetic analysis. The availability of a less invasive procedure would increase the overall efficacy of PGT. The aim of the study was to explore the potential of blastocoele fluid (BF) as an alternative source of embryonic DNA for PGT.

Methods

Collection of BF was performed by aspiration with a fine needle prior to vitrification. BF DNA was subjected to whole-genome amplification (WGA) and analyzed by high-resolution next-generation sequencing (NGS).

Results

A high-quality WGA product was obtained from 8 of 11 (72.7 %) samples. Comparison of matching BF and blastomere samples showed that the genomic representation of sequencing reads was consistently similar with respect to density and regional coverage across the 24 chromosomes. A genome-wide survey of the sample sequencing data also indicated that BF was highly representative of known single gene sequences, and this observation was validated by PCR analyses of ten randomly selected genes, with an overall efficiency of 84 %.

Conclusion

This study provides further evidence that BF is a promising alternative source of DNA for PGT.

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Titel: Molecular analysis of DNA in blastocoele fluid using next-generation sequencing
verfasst von: Yixin Zhang
Na Li
Li Wang
Huiying Sun
Minyue Ma
Hui Wang
Xiaofei Xu
Wenke Zhang
Yingyu Liu
David S. Cram
Baofa Sun
Yuanqing Yao
Publikationsdatum: 22.02.2016
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 5/2016
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-016-0667-7

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Molecular analysis of DNA in blastocoele fluid using next-generation sequencing

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