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01.01.2012 | Brief Report

Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs

verfasst von: Vasileios Ntafis, Eftychia Xylouri, Viviana Mari, Maria Papanastassopoulou, Nikolaos Papaioannou, Angelos Thomas, Canio Buonavoglia, Nicola Decaro

Erschienen in: Archives of Virology | Ausgabe 1/2012

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Abstract

In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.

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Titel: Molecular characterization of a canine coronavirus NA/09 strain detected in a dog’s organs
verfasst von: Vasileios Ntafis
Eftychia Xylouri
Viviana Mari
Maria Papanastassopoulou
Nikolaos Papaioannou
Angelos Thomas
Canio Buonavoglia
Nicola Decaro
Publikationsdatum: 01.01.2012
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 1/2012
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-1141-6

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