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Erschienen in: Archives of Virology 10/2019

25.07.2019 | Annotated Sequence Record

Molecular characterization of a new trichovirus from peach in Mexico

verfasst von: Rodolfo De La Torre-Almaráz, Vicente Pallás, Jesús A. Sánchez-Navarro

Erschienen in: Archives of Virology | Ausgabe 10/2019

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Abstract

The complete genome sequence of a trichovirus was obtained from peach samples collected from Mexico and found to be 7985 nucleotides long, excluding the poly(A) tail. Phylogenetic analysis using the complete nucleotide sequence revealed that the virus is a member of the genus Trichovirus and is closely related to peach mosaic virus (PcMV) and cherry mottle leaf virus (CMLV). The highest nucleotide sequence identity was 70% to both PcMV and CMLV, indicating that this virus, which we have tentatively named “peach virus M” (PeVM) should be considered a member of a new trichovirus species. We determined, for the first time, the initiation sites of the subgenomic RNAs (sgRNA) of a trichovirus. The sgRNAs for the movement and coat proteins started with the sequence ‘GAA’, while the smallest one, coding for the nucleotide-binding protein, started with the nucleotides ‘GU’. In all cases, the sgRNAs leader ranged between 113 and 121 nt in length.
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Literatur
1.
Zurück zum Zitat Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2012) Family Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Elsevier, San Diego, pp 920–941 Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2012) Family Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Elsevier, San Diego, pp 920–941
2.
Zurück zum Zitat De La Torre-Almaraz R, Pallas V, Sanchez-Navarro JA (2015) First report of peach latent mosaic viroid in peach trees from Mexico. Plant Dis 99:899CrossRef De La Torre-Almaraz R, Pallas V, Sanchez-Navarro JA (2015) First report of peach latent mosaic viroid in peach trees from Mexico. Plant Dis 99:899CrossRef
3.
Zurück zum Zitat James D, Varga A, Croft H, Rast H, Thompson D, Hayes S (2006) Molecular characterization, phylogenetic relationships, and specific detection of Peach mosaic virus. Phytopathology 96:137–144CrossRefPubMed James D, Varga A, Croft H, Rast H, Thompson D, Hayes S (2006) Molecular characterization, phylogenetic relationships, and specific detection of Peach mosaic virus. Phytopathology 96:137–144CrossRefPubMed
4.
Zurück zum Zitat Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefPubMedPubMedCentral Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefPubMedPubMedCentral
5.
Zurück zum Zitat Cambra M, Flores R, Pallas V, Gentit P, Candresse T (2008) Viruses and viroids. In: Layne DR, Bassi D (eds) The peach: botany, production and uses. CAB International, Wallingford, pp 435–466CrossRef Cambra M, Flores R, Pallas V, Gentit P, Candresse T (2008) Viruses and viroids. In: Layne DR, Bassi D (eds) The peach: botany, production and uses. CAB International, Wallingford, pp 435–466CrossRef
6.
Zurück zum Zitat Maree HJ, Gardner HF, Freeborough MJ, Burger JT (2010) Mapping of the 5’ terminal nucleotides of Grapevine leafroll-associated virus 3 sgRNAs. Virus Res 151:252–255CrossRefPubMed Maree HJ, Gardner HF, Freeborough MJ, Burger JT (2010) Mapping of the 5’ terminal nucleotides of Grapevine leafroll-associated virus 3 sgRNAs. Virus Res 151:252–255CrossRefPubMed
7.
Zurück zum Zitat Sanchez-Navarro JA, Pallas V (1994) Nucleotide-sequence of apple mosaic ilarvirus Rna-4. J Gen Virol 75:1441–1445CrossRefPubMed Sanchez-Navarro JA, Pallas V (1994) Nucleotide-sequence of apple mosaic ilarvirus Rna-4. J Gen Virol 75:1441–1445CrossRefPubMed
8.
Zurück zum Zitat Sanchez-Navarro JA, Aparicio F, Herranz MC, Minafra A, Myrta A, Pallas V (2005) Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. Eur J Plant Pathol 111:77–84CrossRef Sanchez-Navarro JA, Aparicio F, Herranz MC, Minafra A, Myrta A, Pallas V (2005) Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. Eur J Plant Pathol 111:77–84CrossRef
9.
Zurück zum Zitat Sztuba-Solińska J, Stollar V, Bujarski JJ (2011) Subgenomic messenger RNAs: Mastering regulation of (+)-strand RNA virus life cycle. Virology 412:245–255CrossRefPubMed Sztuba-Solińska J, Stollar V, Bujarski JJ (2011) Subgenomic messenger RNAs: Mastering regulation of (+)-strand RNA virus life cycle. Virology 412:245–255CrossRefPubMed
10.
Zurück zum Zitat Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680CrossRefPubMedPubMedCentral Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680CrossRefPubMedPubMedCentral
11.
Zurück zum Zitat Vives MC, Galipienso L, Navarro L, Moreno P, Guerri J (2002) Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus. Virology 295:328–336CrossRefPubMed Vives MC, Galipienso L, Navarro L, Moreno P, Guerri J (2002) Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus. Virology 295:328–336CrossRefPubMed
Metadaten
Titel
Molecular characterization of a new trichovirus from peach in Mexico
verfasst von
Rodolfo De La Torre-Almaráz
Vicente Pallás
Jesús A. Sánchez-Navarro
Publikationsdatum
25.07.2019
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 10/2019
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-019-04358-y

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