Background
Methods
Viruses, strains, vectors, and other significant materials
PCR amplification and plasmid construction
Primer | Primer sequence (5′-3′) | Gene | Product size (bp) |
---|---|---|---|
P1 | GAATTCATGAAGAGGCGCTGTCTCAAT | DEV US10 | 988 |
P2 | AAGCTTTAGAGTATCAGTCAGAGTCATCGTAG | ||
P3 | CATCCAGTTGCTCCCGT | DEV US10 (for qRT-PCR) | 131 |
P4 | GCGTGACCTAGACAACACC | ||
P5 | CGGGCATCGCTGACA | Duck β-actin gene | 177 |
P6 | GGATTCATCATACTCCTGCTTGCT | ||
P7 | AAGATGCTATGCTGCTAATA | DEV UL55 | 740 |
P8 | CTGTTCGATCTTTACTATTA |
Prokaryotic expression
Preparation and identification of the polyclonal antibody
Real-time PCR (RT-PCR)
Pharmacological inhibition tests
Indirect immunofluorescence assay (IFA)
Virion purification
Western blotting
Mass spectrometry
Results
Expression, identification, and purification of recombinant DEV US10
Purification and verification of US10 antibodies
Kinetics of DEV US10
Subcellular localization of DEV US10
US10 Protein is a component of the virion
Protein | Description | Score | Mass | Matches | Sequences | emPAI | NCBI Accession |
---|---|---|---|---|---|---|---|
UL44 | glycoprotein C | 97 | 47,836 | 6 (3) | 6 (3) | 0.22 | AJG04885 |
US10 | Virion protein | 23 | 34,402 | 4 (1) | 4 (1) | 0.10 | AGW24857 |