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Journal of Cancer Research and Clinical Oncology

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01.02.2009 | Original Paper

Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker

verfasst von: Yun-Ji Kim, Jae-Won Huh, Dae-Soo Kim, Min-In Bae, Ja-Rang Lee, Hong-Seok Ha, Kung Ahn, Tae-Oh Kim, Geun-Am Song, Heui-Soo Kim

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 2/2009

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Abstract

Purpose

DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer.

Methods

RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript.

Results

We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples.

Conclusion

Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.

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Titel: Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker
verfasst von: Yun-Ji Kim
Jae-Won Huh
Dae-Soo Kim
Min-In Bae
Ja-Rang Lee
Hong-Seok Ha
Kung Ahn
Tae-Oh Kim
Geun-Am Song
Heui-Soo Kim
Publikationsdatum: 01.02.2009
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 2/2009
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-008-0445-8

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Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker

Abstract

Purpose

Methods

Results

Conclusion

Neu im Fachgebiet Onkologie

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Adjuvante Brustkrebstherapie: Doppelt hält besser

HNO-Op. auch mit über 90?

Manche Gestagene können das Risiko für Meningeome erhöhen

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

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